Vaccinia Virus Vectors

Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

1986 • 2,118 citations

Smallpox and its eradication

1989 • 1,365 citations

HIV-specific cytotoxic T lymphocytes in seropositive individuals

1987 • 797 citations

Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques.

1985 • 789 citations

Vaccinia virus: a selectable eukaryotic cloning and expression vector.

1982 • 663 citations

General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes

1984 • 652 citations

Complications of Smallpox Vaccination, 1968

1969 • 545 citations

Influenza A virus nucleoprotein is a major target antigen for cross-reactive anti-influenza A virus cytotoxic T lymphocytes.

1985 • 526 citations

HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling an oncogene product

1987 • 523 citations

Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones.

1986 • 513 citations

Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.

1982 • 506 citations

Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype

1985 • 447 citations

HIV-1 gag-specific cytotoxic T lymphocytes defined with recombinant vaccinia virus and synthetic peptides

1988 • 447 citations

Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen

1983 • 422 citations

Structure of vaccinia virus late promoters

1989 • 413 citations

Disseminated Vaccinia in a Military Recruit with Human Immunodeficiency Virus (HIV) Disease

1987 • 412 citations

Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.

1987 • 409 citations

Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.

1989 • 401 citations

Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene.

1984 • 379 citations

Immunization of chimpanzees confers protection against challenge with human immunodeficiency virus.

1991 • 357 citations

Defective presentation to class I-restricted cytotoxic T lymphocytes in vaccinia-infected cells is overcome by enhanced degradation of antigen.

1988 • 331 citations

Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors

1988 • 317 citations

Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity.

1986 • 317 citations

Infectious poxvirus vectors have capacity for at least 25 000 base pairs of foreign DNA

1983 • 311 citations

Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters.

1983 • 296 citations

Live recombinant vaccinia virus protects chimpanzees against hepatitis B

1984 • 290 citations

Construction of live vaccines using genetically engineered poxviruses: biological activity of vaccinia virus recombinants expressing the hepatitis B virus surface antigen and the herpes simplex virus glycoprotein D.

1984 • 287 citations

Oligonucleotide sequence signaling transcriptional termination of vaccinia virus early genes.

1987 • 280 citations

Vaccinia Virus Expression Vectors

1987 • 272 citations

HIV-1 Reverse Transcriptase Is a Target for Cytotoxic T Lymphocytes in Infected Individuals

1988 • 270 citations

Identification of viral molecules recognized by influenza-specific human cytotoxic T lymphocytes.

1987 • 251 citations

Expression of the HTLV-III envelope gene by a recombinant vaccinia virus

1986 • 240 citations

Deletion of the vaccinia virus growth factor gene reduces virus virulence

1988 • 238 citations

Oral vaccination of the fox against rabies using a live recombinant vaccinia virus

1986 • 238 citations

Recombinant vaccinia virus primes and stimulates influenza haemagglutinin-specific cytotoxic T cells

1984 • 234 citations

Biological and immunological properties of human immunodeficiency virus type 1 envelope glycoprotein: analysis of proteins with truncations and deletions expressed by recombinant vaccinia viruses

1991 • 231 citations

Oral immunization and protection of raccoons (Procyon lotor) with a vaccinia-rabies glycoprotein recombinant virus vaccine.

1986 • 230 citations

Vaccinia Virus Recombinants: Expression of VSV Genes and Protective Immunization of Mice and Cattle

1985 • 228 citations

Construction of live vaccines by using genetically engineered poxviruses: biological activity of recombinant vaccinia virus expressing influenza virus hemagglutinin.

1983 • 225 citations

Human immunodeficiency virus-like particles produced by a vaccinia virus expression vector.

1989 • 223 citations

Prevention of vaccinia virus infection in imiminodeficient mice by vector-directed IL-2 expression

1987 • 216 citations

Vaccinia Virus Recombinant Expressing Herpes Simplex Virus Type 1 Glycoprotein D Prevents Latent Herpes in Mice

1985 • 210 citations

Recombinant fowlpox virus inducing protective immunity in non-avian species

1988 • 209 citations

Antigenic subunits of Hantaan virus expressed by baculovirus and vaccinia virus recombinants

1990 • 205 citations

Japanese encephalitis virus-vaccinia recombinants produce particulate forms of the structural membrane proteins and induce high levels of protection against lethal JEV infection

1991 • 202 citations

Structure of vaccinia virus early promoters

1989 • 202 citations

Immunization of mice with recombinant vaccinia virus expressing authentic dengue virus nonstructural protein NS1 protects against lethal dengue virus encephalitis

1990 • 198 citations

Recovery of immunodeficient mice from a vaccinia virus/IL-2 recombinant infection

1987 • 198 citations

Effect of immunization with a vaccinia-HIV env recombinant on HIV infection of chimpanzees

1987 • 197 citations

Cytoplasmic expression system based on constitutive synthesis of bacteriophage T7 RNA polymerase in mammalian cells.

1990 • 195 citations

REGULATION OF VACCINIA VIRUS TRANSCRIPTION

1990 • 194 citations

Expression of AIDS virus envelope gene in recombinant vaccinia viruses

1986 • 192 citations

Recombinant Vaccinia Virus: Immunization Against Multiple Pathogens

1985 • 192 citations

An endogenous processing pathway in vaccinia virus-infected cells for presentation of cytoplasmic antigens to class II-restricted T cells.

1990 • 188 citations

A group specific anamnestic immune reaction against HIV-1 induced by a candidate vaccine against AIDS

1988 • 183 citations

Removal of cryptic poxvirus transcription termination signals from the human immunodeficiency virus type 1 envelope gene enhances expression and immunogenicity of a recombinant vaccinia virus

1990 • 172 citations

Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia viruses

1987 • 166 citations

Cloning and expression of foreign genes in vaccinia virus, using a host range selection system

1989 • 151 citations

Recognition of Influenza A Virus Nucleoprotein by Human Cytotoxic T Lymphocytes

1986 • 151 citations

A nonstructural viral protein expressed by a recombinant vaccinia virus protects against lethal cytomegalovirus infection

1988 • 151 citations

Binding region for human immunodeficiency virus (HIV) and epitopes for HIV-blocking monoclonal antibodies of the CD4 molecule defined by site-directed mutagenesis.

1988 • 150 citations

Temporal regulation of influenza hemagglutinin expression in vaccinia virus recombinants and effects on the immune response

1986 • 149 citations

Human immunodeficiency virus-like, nonreplicating, gag-env particles assemble in a recombinant vaccinia virus expression system

1990 • 144 citations

Protection of Cattle Against Rinderpest with Vaccinia Virus Recombinants Expressing the HA or F Gene

1988 • 142 citations

T-Lymphocyte Priming and Protection Against Friend Leukemia by Vaccinia-RetrovirusenvGene Recombinant

1986 • 141 citations

One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription.

1985 • 139 citations

Effect of the recombinant vaccinia viruses that express HTLV-I envelope gene on HTLV-I infection.

1987 • 134 citations

Structure and stability of mRNA synthesized by vaccinia virus-encoded bacteriophage T7 RNA polymerase in mammalian cells

1989 • 133 citations

Vaccination and protection from a lethal viral infection: Identification, incorporation, and use of a cytotoxic T lymphocyte glycoprotein epitope

1990 • 132 citations

Expression of the fusion protein of human respiratory syncytial virus from recombinant vaccinia virus vectors and protection of vaccinated mice

1987 • 132 citations

Expression of the F and HN glycoproteins of human parainfluenza virus type 3 by recombinant vaccinia viruses: contributions of the individual proteins to host immunity

1987 • 129 citations

A dominant selectable marker for the construction of recombinant poxviruses

1988 • 129 citations

Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants.

1985 • 128 citations

Anti‐viral protection and prevention of lymphocytic choriomeningitis or of the local footpad swelling reaction in mice by immunization with vaccinia‐recombinant virus expressing LCMV‐WE nucleoprotein or glycoprotein

1989 • 123 citations

Protection of rhesus monkeys from fatal Lassa fever by vaccination with a recombinant vaccinia virus containing the Lassa virus glycoprotein gene.

1989 • 122 citations

Anti-influenza virus cytotoxic T lymphocytes recognize the three viral polymerases and a nonstructural protein: responsiveness to individual viral antigens is major histocompatibility complex controlled

1987 • 121 citations

Human respiratory syncytial virus glycoprotein G expressed from a recombinant vaccinia virus vector protects mice against live-virus challenge

1986 • 120 citations

New vaccinia virus recombination plasmids incorporating a synthetic late promoter for high level expression of foreign proteins

1990 • 119 citations

Immunodeficient mice recover from infection with vaccinia virus expressing interferon‐γ

1990 • 117 citations

Resistance to human respiratory syncytial virus (RSV) infection induced by immunization of cotton rats with a recombinant vaccinia virus expressing the RSV G glycoprotein.

1986 • 117 citations

Cytoplasmic transcription system encoded by vaccinia virus

1991 • 114 citations

Recognition of pre-processed endogenous antigen by class I but not class II MHC-restricted T cells

1989 • 113 citations

Interferon gamma is involved in the recovery of athymic nude mice from recombinant vaccinia virus/interleukin 2 infection.

1990 • 110 citations

Regulation of expression and nucleotide sequence of a late vaccinia virus gene

1984 • 106 citations

Protection Against Streptococcal Pharyngeal Colonization with a Vaccinia: M Protein Recombinant

1989 • 105 citations

Mapping and insertional mutagenesis of a vaccinia virus gene encoding a 13,800-Da secreted protein

1989 • 104 citations

Elevated natural killer cell responses in mice infected with recombinant vaccinia virus encoding murine IL-2.

1990 • 103 citations

Protection of mice from fatal measles encephalitis by vaccination with vaccinia virus recombinants encoding either the hemagglutinin or the fusion protein.

1988 • 102 citations

Tumour prevention and rejection with recombinant vaccinia

1987 • 101 citations

Deletion of 55 open reading frames from the termini of vaccinia virus

1991 • 100 citations

Passive transfer of respiratory syncytial virus (RSV) antiserum suppresses the immune response to the RSV fusion (F) and large (G) glycoproteins expressed by recombinant vaccinia viruses

1988 • 99 citations

Recombinant vaccinia virus vaccine against the human melanoma antigen p97 for use in immunotherapy.

1988 • 98 citations

Recombinant vaccinia virus expressing epstein‐barr virus glycoprotein gp340 protects cottontop tamarins against EB virus‐induced malignant lymphomas

1988 • 98 citations

VACCINIA RECOMBINANT EXPRESSING LASSA-VIRUS INTERNAL NUCLEOCAPSID PROTEIN PROTECTS GUINEAPIGS AGAINST LASSA FEVER

1987 • 98 citations

Effective tumor immunotherapy directed against an oncogene-encoded product using a vaccinia virus vector.

1987 • 98 citations

AIDS virus env Protein Expressed from a Recombinant Vaccinia Virus

1986 • 97 citations

Mice immunized with recombinant vaccinia virus expressing dengue 4 virus structural proteins with or without nonstructural protein NS1 are protected against fatal dengue virus encephalitis

1989 • 97 citations

Contribution of measles virus fusion protein in protective immunity: anti-F monoclonal antibodies neutralize virus infectivity and protect mice against challenge

1990 • 97 citations

Plasmodium knowlesi Sporozoite Antigen: Expression by Infectious Recombinant Vaccinia Virus

1984 • 96 citations

Surface expression of viral glycoproteins is polarized in epithelial cells infected with recombinant vaccinia viral vectors.

1986 • 95 citations

Vaccination against tumor cells expressing breast cancer epithelial tumor antigen.

1990 • 95 citations

Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus

1981 • 95 citations

Induction of protective immunity in animals vaccinated with recombinant vaccinia viruses that express PreM and E glycoproteins of Japanese encephalitis virus

1990 • 94 citations

Expression of herpes simplex virus 1 glycoprotein B by a recombinant vaccinia virus and protection of mice against lethal herpes simplex virus 1 infection.

1987 • 94 citations

Recombinant Vaccinia Viruses As Vectors for Studying T Lymphocyte Specificity and Function

1990 • 94 citations

Evaluation of pseudorabies virus glycoprotein gp50 as a vaccine for Aujeszky's disease in mice and swine: expression by vaccinia virus and Chinese hamster ovary cells

1987 • 92 citations

Successful oral rabies vaccination of raccoons with raccoon poxvirus recombinants expressing rabies virus glycoprotein

1988 • 91 citations

Expression of Sindbis virus structural proteins via recombinant vaccinia virus: synthesis, processing, and incorporation into mature Sindbis virions

1985 • 89 citations

Vaccinia viruses as vectors for vaccine antigens

1986 • 88 citations

Vaccinia virus vectors utilizing the β-galactosidase assay for rapid selection of recombinant viruses and measurement of gene expression

1986 • 84 citations

Cell-mediated immune responses to influenza virus antigens expressed by vaccinia virus recombinants

1986 • 83 citations

Regulated expression of foreign genes in mammalian cells under the control of coliphage T3 RNA polymerase and lac repressor.

1989 • 83 citations

The Roles of Influenza Virus Haemagglutinin and Nueleoprotein in Protection: Analysis Using Vaccinia Virus Recombinants

1987 • 82 citations

Expression and Cell Type—Specific Processing of Human Preproenkephalin with a Vaccinia Recombinant

1986 • 81 citations

Immune response in skunks to a vaccinia virus recombinant expressing the rabies virus glycoprotein.

1987 • 81 citations

Protection of guinea pigs from lassa fever by vaccinia virus recombinants expressing the nucleoprotein or the envelope glycoproteins of lassa virus

1989 • 79 citations

Recognition of cloned influenza virus hemagglutinin gene products by cytotoxic T lymphocytes

1986 • 79 citations

Immunization with a vaccinia recombinant expressing the F protein protects Rabbits from challenge with a lethal dose of rinderpest virus

1989 • 78 citations

Genetic studies with mammalian poxviruses

1958 • 78 citations

Selective transcription of vaccinia virus genes in template dependent soluble extracts of infected cells

1983 • 76 citations

Construction of a recombinant vaccinia virus expressing the Lassa virus glycoprotein gene and protection of guinea pigs from a lethal Lassa virus infection

1988 • 74 citations

Expression of the major glycoprotein G of human respiratory syncytial virus from recombinant vaccinia virus vectors.

1986 • 73 citations

Characterization of human immunodeficiency virus gag/pol gene products expressed by recombinant vaccinia viruses

1988 • 71 citations

Insertional inactivation of the large subunit of ribonucleotide reductase encoded by vaccinia virus is associated with reduced virulence in vivo

1990 • 69 citations

A poxvirus-derived vector that directs high levels of expression of cloned genes in mammalian cells.

1988 • 69 citations

Deletion of a 9,000-base-pair segment of the vaccinia virus genome that encodes nonessential polypeptides

1981 • 66 citations

Large-Scale Production and Purification of a Vaccinia Recombinant-Derived HIV-1 gp160 and Analysis of Its Immunogenicity

1989 • 65 citations

Class I MHC can present an endogenous peptide to cytotoxic T lymphocytes.

1989 • 62 citations

Effects and virulences of recombinant vaccinia viruses derived from attenuated strains that express the human T-cell leukemia virus type I envelope gene

1988 • 62 citations

The Origins of Vaccinia Virus

1977 • 61 citations

A New Mutant of Dermovaccinia Virus

1961 • 61 citations

Anchoring a Secreted Plasmodium Antigen on the Surface of Recombinant Vaccinia Virus-Infected Cells Increases Its Immunogenicity

1986 • 60 citations

Protection of chickens from lethal influenza infection by vaccinia-expressed hemagglutinin

1988 • 60 citations

Primary Multiplication Site of the Vaccinia-rabies Glycoprotein Recombinant Virus Administered to Foxes by the Oral Route

1990 • 59 citations

Recombinant vaccinia viruses carrying the N gene of human respiratory syncytial virus: studies of gene expression in cell culture and immune response in mice

1987 • 56 citations

Protection of Guinea Pigs from Primary and Recurrent Herpes Simplex Virus (HSV) Type 2 Cutaneous Disease with Vaccinia Virus Recombinants Expressing HSV Glycoprotein D

1987 • 54 citations

Cloning Vectors Derived from Animal Viruses

1983 • 54 citations

High level gene expression in mammalian cells by a nuclear T7-phage RNA polymerase

1989 • 54 citations

Comparison of the relative roles of the F and HN surface glycoproteins of the paramyxovirus simian virus 5 in inducing protective immunity

1987 • 54 citations

A 15 amino acid fragment of influenza nucleoprotein synthesized in the cytoplasm is presented to class I-restricted cytotoxic T lymphocytes.

1989 • 51 citations

Coexpression by vaccinia virus recombinants of equine herpesvirus 1 glycoproteins gp13 and gp14 results in potentiated immunity

1990 • 50 citations

Anchor sequence-dependent endogenous processing of human immunodeficiency virus 1 envelope glycoprotein gp160 for CD4+ T cell recognition.

1990 • 49 citations

Mechanism of Immunity to Influenza: Maternal and Passive Neonatal Protection Following Immunization of Adult Ferrets with a Live Vaccinia-Influenza Virus Haemagglutinin Recombinant but Not with Recombinants Containing Other Influenza Virus Proteins

1989 • 48 citations

Recombinant vaccinia virus/Venezuelan equine encephalitis (VEE) virus protects mice from peripheral VEE virus challenge

1988 • 47 citations

Vaccinia virus recombinants expressing rabiesvirus glycoprotein protect against rabies

1987 • 46 citations

Evaluation in non-human primates of the safety, immunogenicity and efficacy of recombinant vaccinia viruses expressing the F or G glycoprotein of respiratory syncytial virus

1988 • 46 citations

Immunization with vaccinia virus recombinants that express the surface glycoproteins of human parainfluenza virus type 3 (PIV3) protects patas monkeys against PIV3 infection

1988 • 44 citations

Successful vaccination with a polyvalent live vector despite existing immunity to an expressed antigen

1988 • 44 citations

The immunogenicity of VP7, a rotavirus antigen resident in the endoplasmic reticulum, is enhanced by cell surface expression

1990 • 38 citations

Efficacy of Influenza Haemagglutinin and Nucleoprotein as Protective Antigens against Influenza Virus Infection in Mice

1988 • 38 citations

Expression of polyomavirus virion proteins by a vaccinia virus vector: association of VP1 and VP2 with the nuclear framework

1987 • 37 citations

Anchoring a secreted plasmodium antigen on the surface of recombinant vaccinia virus-infected cells increases its immunogenicity.

1986 • 37 citations

Attenuation and immunogenicity in primates of vaccinia virus recombinants expressing human interleukin-2

1990 • 37 citations

Effectiveness of enteric immunization in the development of secretory immunoglobulin A response and the outcome of infection with respiratory syncytial virus

1991 • 37 citations

Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants

1989 • 32 citations

AMOUNT AND DURATION OF IMMUNITY INDUCED BY INTRADERMAL INOCULATION OF CULTURED VACCINE VIRUS

1939 • 32 citations

Regulated expression of nuclear genes by T3 RNA polymerase and lac repressor, using recombinant vaccinia virus vectors

1990 • 27 citations

Herpes Simplex Virus Type 1-Vaccinia Virus Recombinant Expressing Glycoprotein B: Protection from Acute and Latent Infection

1988 • 27 citations

Transport to the cell surface of a peptide sequence attached to the truncated C terminus of an N-terminally anchored integral membrane protein.

1988 • 25 citations

Response of monkeys to vaccination with recombinant vaccinia virus which coexpress HIV gpl60 and human interleukin‐2

1990 • 20 citations

A Novel Approach for the Production of Monoclonal Antibodies Using Infectious Vaccinia Virus Recombinants

1987 • 19 citations

Antigen-presenting Capacity of Epidermal Cells Infected with Vaccinia Virus Recombinants Containing the Herpes Simplex Virus Glycoprotein D, and Protective Immunity

1989 • 17 citations