Subforms and In Vitro Reconstitution of the NAD-Reducing Hydrogenase of Alcaligenes eutrophus

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Experiments in molecular genetics

1972 • 28,260 citations

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

1983 • 7,336 citations

Crystal structure of the nickel–iron hydrogenase from Desulfovibrio gigas

1995 • 1,508 citations

The structure and mechanism of iron-hydrogenases

1990 • 748 citations

Structure of the [NiFe] Hydrogenase Active Site: Evidence for Biologically Uncommon Fe Ligands

1996 • 657 citations

Wide host range cloning vectors: A cosmid clone bank of an Agrobacterium Ti plasmid

1982 • 568 citations

Nickel hydrogenases: in search of the active site

1994 • 445 citations

Microbial Growth on C1 Compounds

1996 • 438 citations

A practical guide to molecular cloning

1987 • 360 citations

Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16

1976 • 346 citations

The Gene Locus of the Proton-translocating NADH : Ubiquinone Oxidoreductase in Escherichia coli

1993 • 327 citations

Structure-function relationships among the nickel-containing hydrogenases

1992 • 250 citations

MOLECULAR BIOLOGY OF HYDROGEN UTILIZATION IN AEROBIC CHEMOLITHOTROPHS

1993 • 231 citations

The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties

1979 • 221 citations

Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16

1990 • 209 citations

Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenase

1991 • 177 citations

The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation

1994 • 168 citations

Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus

1981 • 161 citations

Molecular Biological Analysis of a Bidirectional Hydrogenase from Cyanobacteria

1995 • 153 citations

Characterization of an operon encoding an NADP-reducing hydrogenase in Desulfovibrio fructosovorans

1995 • 136 citations

Sequence analysis of an operon of a NAD(P)-reducing nickel hydrogenase from the cyanobacterium Synechocystis sp. PCC 6803 gives additional evidence for direct coupling of the enzyme to NAD(P)H-dehydrogenase (complex 1)

1996 • 119 citations

Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus

1982 • 116 citations

Content and localization of FMN, Fe‐S clusters and nickel in the NAD‐linked hydrogenase of Nocardia opaca 1b

1984 • 107 citations

The Alcaligenes eutrophus membrane-bound hydrogenase gene locus encodes functions involved in maturation and electron transport coupling

1996 • 106 citations

A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16

1992 • 103 citations

Intimate relationships of the large and the small subunits of all nickel hydrogenases with two nuclear-encoded subunits of mitochondrial NADH: ubiquinone oxidoreductase

1993 • 97 citations

Functional and Structural Role of the Cytochrome b Subunit of the Membrane‐Bound Hydrogenase Complex of Alcaligenes Eutrophus H16

1997 • 85 citations

Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1b

1984 • 79 citations

Studies on a gram-positive hydrogen bacterium, Nocardia opaca 1 b

1974 • 73 citations

hyp Gene Products in Alcaligenes Eutrophus are part of a Hydrogenase‐Maturation System

1996 • 66 citations

[5] Nickel-iron hydrogenase

1994 • 65 citations

Cloning, molecular analysis and insertional mutagenesis of the bidirectional hydrogenase genes from the cyanobacterium Anacystis nidulans

1996 • 59 citations

Carboxyl-terminal processing of the cytoplasmic NAD-reducing hydrogenase of Alcaligenes eutrophus requires the hoxW gene product

1996 • 59 citations

Genes encoding the NAD-reducing hydrogenase ofRhodococcus opacus MR11

1997 • 57 citations

A Practical Guide to Molecular Cloning (2nd ed)

1989 • 54 citations

C‐Terminal Extension of the H₂‐Activating Subunit, HoxH, Directs Maturation of the NAD‐Reducing Hydrogenase in Alcaligenes Eutrophus

1997 • 35 citations

The NAD-linked soluble hydrogenase from Alcaligenes eutrophus H16: detection and characterization of EPR signals deriving from nickel and flavin

1996 • 32 citations

Characterization of a native subunit of the NAD-linked hydrogenase isolated from a mutant of Alcaligenes eutrophus H16

1986 • 27 citations

Location, catalytic activity, and subunit composition of NAD-reducing hydrogenases of some Alcaligenes strains and Rhodococcus opacus MR22.

1997 • 25 citations

Location, catalytic activity, and subunit composition of NAD-reducing hydrogenases of some Alcaligenes strains and Rhodococcus opacus MR22

1997 • 22 citations

Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm

1995 • 17 citations

Effect of molecular hydrogen on histidine utilization by Alcaligenes eutrophus

1982 • 15 citations

EPR and M�ssbauer spectroscopic studies on the tetrameric, NAD-linked hydrogenase of Nocardia opaca 1b and its two dimers: 1. The ??-dimer?a prototype of a simple hydrogenase

1995 • 15 citations

Structural aspects of the soluble NAD-dependent hydrogenase isolated from Alcaligenes eutrophus H16 and from Nocardia opaca 1b

1991 • 14 citations

In Azotobacter vinelandii hydrogenase, substitution of serine for the cysteine residues at positions 62, 65, 294, and 297 in the small (HoxK) subunit affects H2 oxidation [corrected]

1993 • 12 citations

Hydrogen Oxidation by Alcaligenes

1996 • 9 citations