Gene amplification in methotrexate-resistant mouse cells

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

1977 • 5,640 citations

A simple technique for demonstrating centromeric heterochromatin

1972 • 4,206 citations

A membrane-filter technique for the detection of complementary DNA

1966 • 3,996 citations

Isolation of High‐Molecular‐Weight DNA from Mammalian Cells

1973 • 1,723 citations

Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis

1973 • 1,616 citations

Transformation of mammalian cells with genes from procaryotes and eucaryotes

1979 • 1,474 citations

Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure

1979 • 1,403 citations

Methylmercury as a reversible denaturing agent for agarose gel electrophoresis

1976 • 1,323 citations

The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I

1980 • 1,114 citations

Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

1978 • 972 citations

Enhanced autoradiographic detection of ³²P and ¹²⁵I using intensifying screens and hypersensitized film

1977 • 947 citations

An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH

1980 • 872 citations

Sequences at the somatic recombination sites of immunoglobulin light-chain genes

1979 • 859 citations

Tissue-specific DNA cleavages in the globin chromatin domain introduced by DNAase I

1980 • 486 citations

Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells.

1979 • 484 citations

The use of proteolytic enzymes for the mapping of structural rearrangements in the chromosomes of man

1972 • 461 citations

The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence

1979 • 445 citations

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

1978 • 426 citations

Metaphase Chromosome Anomaly: Association with Drug Resistance and Cell-Specific Products

1976 • 396 citations

Primary activation of the vitellogenin gene in the rooster.

1977 • 353 citations

Gene Amplification and Drug Resistance in Cultured Murine Cells

1978 • 317 citations

Rapid visualization of protein bands in preparative SDS-polyacrylamide gels

1979 • 310 citations

Studies in Antibody Response of Mice to Tumour Inoculation

1950 • 298 citations

Size heterogeneity in the 3′ end of dihydrofolate reductase messenger RNAs in mouse cells

1980 • 288 citations

A precursor of globin messenger RNA

1976 • 254 citations

DNA Sequences Mediating Class Switching in α-Immunoglobulins

1980 • 243 citations

Altering Genotype and Phenotype by DNA-Mediated Gene Transfer

1980 • 242 citations

Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cells

1976 • 237 citations

Murine Lymphocyte Surface Antigens

1979 • 228 citations

Structure and genomic organization of the mouse dihydrofolate reductase gene

1980 • 218 citations

Relation of Folic Acid Reductase to Amethopterin Resistance in Cultured Mammalian Cells

1961 • 210 citations

A new method for the purification and identification of covalently closed circular DNA molecules

1978 • 210 citations

The analysis of malignancy by cell fusion. VII. cytogenetic analysis of hybrids between malignant and diploid cells and of tumours derived from them

1977 • 201 citations

Double Minute Chromosomes and the Homogeneously Staining Regions in Chromosomes of a Human Neuroblastoma Cell Line

1977 • 195 citations

A simple, general procedure for purifying restriction endonucleases

1977 • 192 citations

Correlation of dihydrofolate reductase elevation with gene amplification in a homogeneously staining chromosomal region in L5178Y cells.

1979 • 185 citations

Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate

1975 • 164 citations

Increased levels of folic acid reductase as a mechanism of resistance to amethopterin in leukemic cells

1961 • 152 citations

DNA methylation: correlation with DNase I sensitivity of chicken ovalbumin and conalbumin chromatin

1979 • 130 citations

Isolated mammalian metaphase chromosomes

1967 • 125 citations

Antifolate-resistant Chinese hamster cells. Evidence for dihydrofolate reductase gene amplification among independently derived sublines overproducing different dihydrofolate reductases.

1980 • 100 citations

Regulation of folate reductase synthesis in sensitive and methotrexate-resistant sarcoma 180 cells. In vitro translation and characterization of folate reductase mRNA.

1976 • 97 citations

Polyoma virus and cyclic AMP-mediated control of dihydrofolate reductase mRNA abundance in methotrexate-resistant mouse fibroblasts.

1979 • 89 citations

An Electron Spin Resonance Study of Microsomal Fe

1967 • 88 citations

Specifically altered metaphase chromosomes in antifolate-resistant Chinese hamster cells that overproduce dihydrofolate reductase

1980 • 88 citations

Chicken macrochromosomes contain an endogenous provirus and microchromosomes contain sequences related to the transforming gene of ASV

1977 • 75 citations

Elevated dihydrofolate reductase messenger RNA levels in methotrexate-resistant BHK cells

1976 • 74 citations

Satellite DNA in large marker chromosomes of methotrexate-resistant mouse cells

1980 • 72 citations

An immunoglobulin heavy-chain gene is altered in two T-cell clones

1980 • 72 citations

Structure and organization of the highly repeated and interspersed 1.3 kb EcoRI - Bg1II sequence family in mice

1980 • 67 citations

Gene amplification in methotrexate-resistant mouse cells

1981 • 66 citations

A new chromosome type replacing the double minutes in a mouse tumor

1978 • 65 citations

Marked increase in ribosomal RNA gene multiplicity in a rat hepatoma cell line

1979 • 65 citations

The development of resistance to methotrexate in a mouse melanoma cell line

1979 • 63 citations

A family of moderately repetitive sequences in mouse DNA

1980 • 59 citations

DOUBLE-MINUTES - A CHROMOSOMAL ABERRATION IN ROUS SARCOMAS IN MICE

2009 • 59 citations

Amplification and rearrangement of integrated SV40 DNA sequences accompany the selection of anchorage-independent transformed mouse cells

1980 • 59 citations

In vitro construction of bacteriophage λ and plasmid DNA molecules containing DNA fragments from bacteriophage T4

1976 • 45 citations

Ultrastructure of double minutes from a human tumor cell line.

1979 • 39 citations

Gene amplification in methotrexate-resistant mouse cells

1981 • 34 citations

Amethopterin-induced changes in the multiple forms of dihydrofolate reductase from L1210 cells

1970 • 34 citations

A Revolution in Biology

1980 • 32 citations

CYTOGENETICS OF MOUSE LEUKEMIA L1210. I. ASSOCIATION OF A SPECIFIC CHROMOSOME WITH DIHYDROFOLATE REDUCTASE ACTIVITY IN AMETHOPTERIN-TREATED SUBLINES.

1965 • 31 citations

Isolated hapten‐binding receptors of sensitized lymphocytes: V. Cellular origin of receptor molecules

1979 • 26 citations

Loss of Resistance to Methotrexate in L5178Y Mouse Leukemia Grown <italic>In Vitro</italic>

1972 • 20 citations

Chopping and changing in immunoglobulin genes

1980 • 12 citations

Large double minutes with ring-shape and rod-shape

2009 • 11 citations

Topological constraints daring replication I. Generation of catenanes, circular dimers and higher oligomers

1972 • 6 citations

Topological constraints during replication II. Complex forms generated by multiple replication forks or track switching

1972 • 5 citations