Stable intermediates can be trapped during the reversible refolding of urea-denatured rhodanese.

[14]Determination and analysis of urea and guanidine hydrochloride denaturation curves

1986 • 2,265 citations

FLUORESCENCE AND THE LOCATION OF TRYPTOPHAN RESIDUES IN PROTEIN MOLECULES

1973 • 1,159 citations

Urea and Guanidine Hydrochloride Denaturation of Ribonuclease, Lysozyme, α-Chymotrypsin, and b-Lactoglobulin

1974 • 798 citations

Polarization of the fluorescence of macromolecules. 1. Theory and experimental method

1952 • 673 citations

Solvent denaturation

1978 • 383 citations

Kinetic role of a meta-stable native-like two-disulphide species in the folding transition of bovine pancreatic trypsin inhibitor

1984 • 308 citations

Selective binding and solvent denaturation

1987 • 307 citations

The covalent and tertiary structure of bovine liver rhodanese

1978 • 292 citations

Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation

1986 • 275 citations

Unity in Function in the Absence of Consensus in Sequence: Role of Leader Peptides in Export

1989 • 222 citations

Rotational freedom of tryptophan residues in proteins and peptides

1983 • 218 citations

Estimation of the free energy of stabilization of ribonuclease A, lysozyme, alpha-lactalbumin, and myoglobin.

1982 • 171 citations

Equilibrium and kinetics of the unfolding of lysozyme (muramidase) by guanidine hydrochloride

1966 • 141 citations

Detergent-assisted refolding of guanidinium chloride-denatured rhodanese. The effect of lauryl maltoside.

1986 • 127 citations

Use of site-directed mutagenesis to destabilize native apomyoglobin relative to folding intermediates

1989 • 124 citations

The mechanism of folding of globular proteins. Equilibria and kinetics of conformational transitions of penicillinase from Staphylococcus aureus involving a state of intermediate conformation

1976 • 108 citations

Reversible folding of rhodanese

1989 • 78 citations

Effects of sulphate and urea on the stability and reversible unfolding of β-lactamase from Staphylococcus aureus

1985 • 76 citations

Purification of thiosulfate sulfurtransferase by selective immobilization on blue agarose

1978 • 74 citations

Expression of functionaly of α-chymotrypsin. Effects of guanidine hydrochloride and urea in the onset of denaturation

1978 • 68 citations

The high resolution three-dimensional structure of bovine liver rhodanese

1983 • 66 citations

Preprotein conformation: the year's major theme in translocation studies

1988 • 63 citations

Low concentrations of guanidinium chloride expose apolar surfaces and cause differential perturbation in catalytic intermediates of rhodanese.

1986 • 58 citations

Detection, characterization, and quenching of the intrinsic fluorescence of bovine heart cytochrome c oxidase

1986 • 49 citations

Proline peptide isomerization and the reactivation of denatured enzymes

1979 • 47 citations

Depolarization of the intrinsic and extrinsic fluorescence of pepsinogen and pepsin

1970 • 47 citations

Soaking in Cs₂SO₄ reveals a caesium‐aromatic interaction in bovine‐liver rhodanese

1988 • 46 citations

Conformational changes accompany the oxidative inactivation of rhodanese by a variety of reagents.

1987 • 41 citations

Low-molecular Carbohydrates in Algae. II. Synthesis of 1-D-Mannitol Monoacetate and 1,6-D-Mannitol Diacetate.

1953 • 38 citations

The enzyme rhodanese can be reactivated after denaturation in guanidinium chloride.

1986 • 37 citations

The use of intrinsic protein fluorescence to quantitate enzyme-bound persulfide and to measure equilibria between intermediates in rhodanese catalysis.

1983 • 33 citations

Active site modifications quench intrinsic fluorescence of rhodanese by different mechanisms

1986 • 30 citations

Denaturation-induced disulfide formation in the enzyme rhodanese

1976 • 30 citations

Differences in the binding of sulfate, selenate and thiosulfate ions to bovine liver rhodanese, and a description of a binding site for ammonium and sodium ions

1984 • 29 citations

A Fluorescence study of conformational changes induced by substrate and temperature in bovine liver thiosulfate sulfurtransferase

1982 • 29 citations

Effects of metabolite binding to ribulosebisphosphate carboxylase on the activity of the Calvin photosynthesis cycle

1988 • 28 citations

Oxidative Inactivation of Rhodanese by Hydrogen Peroxide Produces States that Show Differential Reactivation

1989 • 26 citations

Enzymic memory. Consequence of conformational mobility

1974 • 26 citations

Sulfhydryl-directed triggering of conformational changes in the enzyme rhodanese.

1988 • 25 citations

Studies on rhodanese synthesis in bovine adrenocortical cells

1985 • 22 citations

Oxidative inactivation of the enzyme rhodanese by reduced nicotinamide adenine dinucleotide.

1986 • 17 citations

Native or nativelike species are transient intermediates in folding of alkaline iso-2 cytochrome c

1986 • 16 citations

A study of the quenching of the intrinsic fluorescence of succinyl-CoA synthetase from Escherichia coli by acrylamide, iodide, and coenzyme A

1983 • 15 citations

Contact versus energy transfer fluorescence quenching in the sulfur substituted form of the enzyme rhodanese: A study using cesium ion resolved emission spectra

1975 • 14 citations

Characterization of rhodanese-tetracyanonickelate. An active site complex that slows sulfur-free rhodanese conversion to inert conformers.

1986 • 6 citations