Cysteine 532 and Cysteine 545 Are the N-ethylmaleimide-reactive Residues of the Neurospora Plasma Membrane H+-ATPase

Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.

1982 • 5,284 citations

Assay of proteins in the presence of interfering materials

1976 • 3,217 citations

Amino-acid sequence of a Ca2+ + Mg2+ -dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence

1985 • 1,126 citations

Ion motive ATPases. I. Ubiquity, properties, and significance to cell function

1987 • 964 citations

Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+- and Ca2+-ATPases

1986 • 801 citations

Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNA

1985 • 762 citations

The proton-translocating ATPase of the fungal plasma membrane

1981 • 493 citations

THE DETERMINATION OF PHOSPHORUS AND PHOSPHATASE WITH N-PHENYL-p-PHENYLENEDIAMINE

1957 • 472 citations

Structure and function of proton translocating ATPase in plasma membranes of plants and fungi

1988 • 464 citations

Molecular cloning of the rat stomach (H+ + K+)-ATPase.

1986 • 463 citations

Molecular cloning of two isoforms of the plasma membrane Ca2+-transporting ATPase from rat brain. Structural and functional domains exhibit similarity to Na+,K+- and other cation transport ATPases.

1988 • 379 citations

Sequence homology between two membrane transport ATPases, the Kdp-ATPase of Escherichia coli and the Ca2+-ATPase of sarcoplasmic reticulum.

1984 • 204 citations

H+-ATPases from mitochondria, plasma membranes, and vacuoles of fungal cells

1986 • 197 citations

Methods in Protein Sequence Analysis

1982 • 190 citations

Amino acid sequence of the plasma membrane ATPase of Neurospora crassa: deduction from genomic and cDNA sequences.

1986 • 166 citations

Prediction of peptide retention times in reversed-phases high-performance liquid chromatography during linear gradient elution

1982 • 149 citations

A Hypothesis for the Role of Dithiol‐Disulfide Interchange in Solute Transport and Energy‐Transducing Processes

1982 • 143 citations

Purification and characterization of the plasma membrane ATPase of Neurospora crassa.

1981 • 138 citations

Primary structure of the Neurospora plasma membrane H+-ATPase deduced from the gene sequence. Homology to Na+/K+-, Ca2+-, and K+-ATPase.

1986 • 124 citations

The primary structure of the calcium ion-transporting adenosine triphosphatase protein of rabbit skeletal sarcoplasmic reticulum. Peptides derived from digestion with cyanogen bromide, and the sequences of three long extramembranous segments

1980 • 110 citations

Mutation of a conserved glycine residue modifies the vanadate sensitivity of the plasma membrane H+-ATPase from Schizosaccharomyces pombe.

1987 • 108 citations

Identification of the phosphorylated intermediate of the Neurospora plasma membrane H+-ATPase as beta-aspartyl phosphate.

1981 • 89 citations

High-performance liquid chromatographic peptide mapping and amino acid analysis in the sub-nanomole range

1986 • 86 citations

Site-directed mutagenesis of cys148 in the lac carrier protein of Escherichia coli

1984 • 85 citations

Removal of dodecyl sulfate from protein solution

1988 • 82 citations

Physical mechanism for regulation of proton solute symport in Escherichia coli.

1982 • 79 citations

Isolation and characterization of plasma membranes from wild type Neurospora crassa.

1981 • 71 citations

Inhibition of the plasma membrane [H+]-ATPase of Neurospora crassa by N-ethylmaleimide. Protection by nucleotides.

1982 • 58 citations

Role of cysteine residues in the lac permease of Escherichia coli

1987 • 58 citations

Selective Modification of Functionally Distinct Sulfhydryl Groups of Sarcoplasmic Reticulum Ca2+, Mg2+-Adenosine Triphosphatase with N-Ethylmaleimide

1980 • 56 citations

Site-directed mutagenesis of cysteine-148 in the Lac permease of Escherichia coli: effect on transport, binding, and sulfhydryl inactivation

1985 • 47 citations

The reaction of sulfhydryl groups of sodium and potassium ion-activated adenosine triphosphatase with N-ethylmaleimide. The relationship between ligand-dependent alterations of nucleophilicity and enzymatic conformational states.

1981 • 44 citations

[14C]N-ethylmaleimide labeling of the plasma membrane [H+]-ATPase of Neurospora crassa.

1983 • 43 citations

The fluorescein isothiocyanate-binding site of the plasma-membrane H+-ATPase of Neurospora crassa.

1988 • 40 citations

Properties of a mutant lactose carrier of Escherichia coli with a Cys¹⁴⁸ → Ser¹⁴⁸ substitution

1985 • 38 citations

Site-specific mutagenesis of cysteine 148 to serine in the lac permease of Escherichia coli.

1986 • 34 citations

The plasma membrane H+-ATPase of Neurospora crassa. Properties of two reactive sulfhydryl groups.

1988 • 15 citations