Expression and purification of functional human 17 alpha-hydroxylase/17,20-lyase (P450c17) in Escherichia coli. Use of this system for study of a novel form of combined 17 alpha-hydroxylase/17,20-lyase deficiency.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Antibodies, a laboratory manual

1989 • 12,929 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Antibodies: A laboratory manual

1989 • 4,532 citations

A general method ofin vitropreparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

1988 • 2,410 citations

High-resolution crystal structure of cytochrome P450cam

1987 • 1,396 citations

Substrate recognition sites in cytochrome P450 family 2 (CYP2) proteins inferred from comparative analyses of amino acid and coding nucleotide sequences.

1992 • 1,168 citations

The P450 Superfamily: Update on New Sequences, Gene Mapping, and Recommended Nomenclature

1991 • 1,092 citations

Conjugation of antibodies with fluorochromes: Modifications to the standard methods

1976 • 757 citations

17-hydroxylation deficiency in man.

1966 • 562 citations

Complete nucleotide sequence of two steroid 21-hydroxylase genes tandemly arranged in human chromosome: a pseudogene and a genuine gene.

1986 • 559 citations

Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli.

1991 • 547 citations

[3] Expression and nitrogen-15 labeling of proteins for proton and nitrogen-15 nuclear magnetic resonance

1989 • 455 citations

Cytochrome P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase): cloning of human adrenal and testis cDNAs indicates the same gene is expressed in both tissues.

1987 • 449 citations

Expression of Bovine 17α-Hydroxylase Cytochrome P-450 cDNA in Nonsteroidogenic (COS 1) Cells

1986 • 389 citations

17α-Hydroxylase/17,20-Lyase Deficiency: From Clinical Investigation to Molecular Definition*

1991 • 387 citations

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1988 • 248 citations

C21 steroid side chain cleavage enzyme from porcine adrenal microsomes. Purification and characterization of the 17 alpha-hydroxylase/C17,20-lyase cytochrome P-450.

1984 • 212 citations

Bovine adrenocortical cytochrome P-450(17 alpha). Regulation of gene expression by ACTH and elucidation of primary sequence.

1986 • 211 citations

ASSIGNMENT OF THE GENE FOR ADRENAL P450cl7 (STEROID 17α-HYDR0XYLASE⁄17,20 LYASE) TO HUMAN CHROMOSOME 10.

1986 • 167 citations

Rat P450_17αfrom Testis: Characterization of a Full-Length cDNA Encoding a Unique Steroid Hydroxylase Capable of Catalyzing Both Δ⁴- and Δ⁵-Steroid-17,20-Lyase Reactions

1989 • 160 citations

Structural Characterization of Normal and Mutant Human Steroid 17α- Hydroxylase Genes: Molecular Basis of One Example of Combined 17α- Hydroxylase/17,20 Lyase Deficiency

1988 • 154 citations

Alcohol-inducible cytochrome P-450IIE1 lacking the hydrophobic NH2-terminal segment retains catalytic activity and is membrane-bound when expressed in Escherichia coli

1991 • 145 citations

Missense mutation serine106—-proline causes 17 alpha-hydroxylase deficiency

1991 • 137 citations

Testicular microsomal cytochrome P-450 for C21 steroid side chain cleavage. Spectral and binding studies.

1981 • 130 citations

Spectrophotometric properties of a triton-clarified steroid 21-Hydroxylase system of adrenocortical microsomes

1965 • 121 citations

High‐level expression of functional human cytochrome P450 1A2 in Escherichia coli

1992 • 111 citations

Nucleotide sequence analysis of murine 21-hydroxylase genes: mutations affecting gene expression.

1986 • 102 citations

Deletion of a phenylalanine in the N-terminal region of human cytochrome P-45017α results in partial combined 17α-hydroxylase/17,20-lyase deficiency

1989 • 97 citations

PROGRESS IN ENDOCRINOLOGY

1953 • 94 citations

Purification and some properties of cytochrome P-450 specific for steroid 17α-hydroxylation and C17C20 bond cleavage from guinea pig adrenal microsomes

1982 • 92 citations

Multiple catalytic properties of the purified and reconstituted cytochrome P-450 (P-450sccII) system of pig testis microsomes.

1984 • 88 citations

Characterization of Complementary Deoxyribonucleic Acid for Human Adrenocortical 17α-Hydroxylase: A Probe for Analysis of 17α-Hydroxylase Deficiency*

1987 • 87 citations

A molecular model for the enzyme cytochrome P45017α, a major target for the chemotherapy of prostatic cancer

1990 • 85 citations

Canadian Mennonites and individuals residing in the Friesland region of the Netherlands share the same molecular basis of 17?-hydroxylase deficiency

1992 • 83 citations

The expression of a catalytically active cholesterol 7 alpha-hydroxylase cytochrome P450 in Escherichia coli.

1991 • 81 citations

Rainbow trout cytochrome P‐450_c17 (17α‐hydroxylase/17,20‐lyase) cDNA cloning, enzymatic properties and temporal pattern of ovarian P‐450_c17 mRNA expression during oogenesis

1992 • 81 citations

A predicted three-dimensional structure of human cytochrome P450: implications for substrate specificity

1991 • 78 citations

The hydrophobic amino-terminal sequence of bovine 17 alpha-hydroxylase is required for the expression of a functional hemoprotein in COS 1 cells.

1991 • 71 citations

Structural analysis of cloned cDNA for mRNA of microsomal cytochrome P-450(C21) which catalyzes steroid 21-hydroxylation in bovine adrenal cortex.

1986 • 70 citations

cDNA cloning and sequence analysis of a chicken gene expressed during the gonadal development and homologous to mammalian cytochrome P-450c17

1988 • 68 citations

Expression in Escherichia coli of Functional Cytochrome P450c17 Lacking Its Hydrophobic Amino-Terminal Signal Anchor

1993 • 64 citations

Dissociation of Hydroxylase and Lyase Activities by Site-Directed Mutagenesis of the Rat P450_17α

1991 • 64 citations

Role of cytochrome b5 in the cytochrome P-450-mediated C21-steroid 17,20-lyase reaction

1982 • 61 citations

Compound heterozygous mutations (Arg 239----stop, Pro 342----Thr) in the CYP17 (P45017 alpha) gene lead to ambiguous external genitalia in a male patient with partial combined 17 alpha-hydroxylase/17,20-lyase deficiency.

1992 • 53 citations

Expression of Bovine Cytochrome P450c17 cDNA inSaccharomyces cerevisiae

1989 • 49 citations

Molecular basis of apparent isolated 17,20-lyase deficiency: compound heterozygous mutations in the C-terminal region (Arg(496) → Cys, Gln(461) → Stop) actually cause combined 17α-hydroxylase/17,20-lyase deficiency

1992 • 48 citations

Purification and Characterization of Recombinant-Expressed Cytochrome P450 2C3 from Escherichia coli: 2C3 Encodes the 6β-Hydroxylase Deficient Form of P450-3b

1993 • 46 citations

Isolation, characterization, and chromosomal mapping of mouse P450 17α-hydroxylase/C17–20 lyase

1991 • 42 citations

Complete amino acid sequence of 21-hydroxylase cytochrome P-450 from porcine adrenal microsomes

1987 • 23 citations

Co-induction of 17α-hydroxylase and c-17,20-lyase activities in primary cultures of bovine adrenocortical cells in response to ACTH treatment

1988 • 18 citations

Purification and properties of steroid 17α-hydroxylase from calf testis

1991 • 14 citations

Deleted Work

1955 • 0 citations