The preparation and characterization of monoclonal antibodies to human complement component C8 and their use in purification of C8 and C8 subunits

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane

1971 • 8,619 citations

[1] Preparation of monoclonal antibodies: Strategies and procedures

1981 • 1,787 citations

Electroimmuno Assay

1972 • 1,689 citations

Complement

1975 • 594 citations

Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.

1984 • 538 citations

Activation of the alternate pathway of human complements by rabbit cells.

1974 • 473 citations

Regulation by membrane sialic acid of β1H-dependent decay-dissociation of amplification C3 convertase of the alternative complement pathway

1978 • 415 citations

Recovery of human neutrophils from complement attack: removal of the membrane attack complex by endocytosis and exocytosis.

1987 • 251 citations

Complement and Bacteria: Chemistry and Biology in Host Defense

1984 • 226 citations

Membrane damage by complement

1983 • 216 citations

Structural/functional similarity between proteins involved in complement- and cytotoxic T-lymphocyte-mediated cytolysis

1986 • 209 citations

Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor

1986 • 203 citations

The sequence and topology of human complement component C9.

1985 • 179 citations

Nucleotide sequence of cDNA and derived amino acid sequence of human complement component C9.

1984 • 160 citations

Membrane attack by complement

1984 • 156 citations

The eighth component of human complement. Purification and physicochemical characterization of its unusual subunit structure.

1980 • 137 citations

Monoclonal antibodies demonstrate protection of polymorphonuclear leukocytes against complement attack

1985 • 123 citations

Complementary DNA and derived amino acid sequence of the .alpha. subunit of human complement protein C8: evidence for the existence of a separate .alpha. subunit messenger RNA

1987 • 112 citations

Activation and control of the complement system.

1986 • 111 citations

Complementary DNA and derived amino acid sequence of the .beta. subunit of human complement protein C8: identification of a close structural and ancestral relationship to the .alpha. subunit and C9

1987 • 106 citations

Ultrastructure of the membrane attack complex of complement: detection of the tetramolecular C9-polymerizing complex C5b-8.

1982 • 106 citations

Solubilization and immune-detection of β-galactosidase hybrid proteins carrying foreign antigenic determinants

1983 • 102 citations

Topological mapping of complement component C9 by recombinant DNA techniques suggests a novel mechanism for its insertion into target membranes.

1987 • 101 citations

Intracellular Ca2+ and cell injury: A paradoxical role of Ca2+ in complement membrane attack

1986 • 99 citations

Stimulation of Ca2+-dependent chemiluminescence in rat polymorphonuclear leucocytes by polystyrene beads and the non-lytic action of complement.

1981 • 88 citations

Immunobiology of the Complement System

1986 • 76 citations

Molecular composition of the tubular structure of the membrane attack complex of complement.

1984 • 74 citations

Solubilization and purification of rat liver 5′-nucleotidase by use of a zwitterionic detergent and a monoclonal-antibody immunoadsorbent

1982 • 72 citations

Complementary DNA cloning of complement C8.beta. and its sequence homology to C9

1987 • 72 citations

The membrane attack complex of complement: C5b-8 complex as accelerator of C9 polymerization.

1985 • 71 citations

Proteolytic modification of human complement protein C9: loss of poly(C9) and circular lesion formation without impairment of function.

1985 • 62 citations

Two types of dysfunctional eighth component of complement (C8) molecules in C8 deficiency in man. Reconstitution of normal C8 from the mixture of two abnormal C8 molecules.

1983 • 62 citations

A mechanism for the insertion of complement component C9 into target membranes

1986 • 61 citations

Immunoaffinity purification of human complement component C9 using monoclonal antibodies

1983 • 58 citations

Role of the beta subunit in interaction of the eighth component of human complement with the membrane-bound cytolytic complex.

1981 • 55 citations

Intracellular free calcium as a pathogen in cell damage initiated by the immune system

1981 • 54 citations

Characterization of monoclonal antibodies directed against human thyroid stimulating hormone

1982 • 52 citations

A ‘dot-immunobinding assay’ on nitrocellulose membrane for the determination of the immunoglobulin class of mouse monoclonal antibodies

1984 • 48 citations

The architecture of complement component C9 and poly(C9).

1985 • 43 citations

Bacterial killing by complement. C9-mediated killing in the absence of C5b-8

1987 • 42 citations

Complement-mediated killing of Escherichia coli: dissipation of membrane potential by a C9-derived peptide

1986 • 40 citations

Analysis of the specific association of the eighth and ninth components of human complement: identification of a direct role for the .alpha. subunit of C8

1985 • 39 citations

Immunolocalization of complement component C9 on necrotic and non-necrotic muscle fibres in myositis using monoclonal antibodies: a primary role of complement in autoimmune cell damage.

1984 • 39 citations

The preparation of monoclonal antibodies to human bone and liver alkaline phosphatase and their use in immunoaffinity purification and in studying these enzymes when present in serum

1987 • 35 citations

Antigenic crossreactivity of the alpha subunit of complement component C8 with the cysteine-rich domain shared by complement component C9 and low density lipoprotein receptor.

1986 • 34 citations

Inhibition of complement-induced [14C]sucrose release by intracellular and extracellular monoclonal antibodies to C9: Evidence that C9 is a transmembrane protein

1984 • 27 citations

The ninth component of human complement (C9). Functional activity of the b fragment.

1986 • 24 citations

Studies of the association of the eighth and ninth components of human complement within the membrane-bound cytolytic complex.

1983 • 23 citations

Effect of Lethal Doses of Complement on the Functional Integrity of Target Enterobacteria

1985 • 22 citations

Methods in complement for clinical immunologists

1986 • 21 citations

Purification of Functional Subunits of the Eighth Component of Human Complement (C8) under Nondenaturing Conditions

1984 • 20 citations