Site-directed mutagenesis of Petunia hybrida 5-enolpyruvylshikimate-3-phosphate synthase: Lys-23 is essential for substrate binding.

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

[2] New M13 vectors for cloning

1983 • 5,402 citations

An improved assay for nanomole amounts of inorganic phosphate

1979 • 2,157 citations

The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase

1980 • 1,022 citations

Synthesis of deoxyoligonucleotides on a polymer support

1981 • 870 citations

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

[36] High-sensitivity sequencing with a gas-phase sequenator

1983 • 670 citations

Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate

1985 • 370 citations

Kinetics of 5‐enolpyruvylshikimate‐3‐phosphate synthase inhibition by glyphosate

1983 • 262 citations

A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate.

1985 • 232 citations

The isolation and nucleotide sequence of the complexAROMlocus ofAspergillus nidulans

1986 • 133 citations

Structure, expression, and evolution of the 5-enolpyruvylshikimate-3-phosphate synthase genes of petunia and tomato.

1988 • 128 citations

The Enzymatic Formation and Isolation of 3-Enolpyruvylshikimate 5-Phosphate

1964 • 108 citations

Evaluation of 5-enolpyruvoylshikimate-3-phosphate synthase substrate and inhibitor binding by stopped-flow and equilibrium fluorescence measurements

1988 • 103 citations

On the Mechanism of 5-Enolpyruvylshikimate 3-Phosphate Synthetase

1971 • 87 citations

Mechanism of enolpyruvylshikimate-3-phosphate synthase exchange of phosphoenolpyruvate with solvent protons

1983 • 72 citations

The purification of 5‐enolpyruvylshikimate 3‐phosphate synthase from an overproducing strain of Escherichia coli

1984 • 68 citations

The complete amino acid sequence of Escherichia coli 5‐enolpyruvylshikimate 3‐phosphate synthase

1984 • 65 citations

Identification of the reactive cysteines of Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthase and their nonessentiality for enzymatic catalysis.

1988 • 51 citations

5-Enolpyruvyl shikimate 3-phosphate synthase from Escherichia coli. Identification of Lys-22 as a potential active site residue.

1988 • 39 citations

The fate of the hydrogens of phosphoenolpyruvate in the reaction catalyzed by 5-enolpyruvylshikimate-3-phosphate synthase. Isotope effects and isotope exchange.

1982 • 34 citations

Evidence for a reactive gamma-carboxyl group (Glu-418) at the herbicide glyphosate binding site of 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli.

1988 • 17 citations

Reaction of 5-enol-pyruvoylshikimate-3-phosphate synthase with diethyl pyrocarbonate: Evidence for an essential histidine residue

1987 • 17 citations