Genetic Immunization Against the Human Thyrotropin Receptor Causes Thyroiditis and Allows Production of Monoclonal Antibodies Recognizing the Native Receptor

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Direct Gene Transfer into Mouse Muscle in Vivo

1990 • 3,985 citations

Interferon-γ and B Cell Stimulatory Factor-1 Reciprocally Regulate Ig Isotype Production

1987 • 1,936 citations

Antibodies to major histocompatibility antigens produced by hybrid cell lines

1977 • 1,727 citations

Genetic immunization is a simple method for eliciting an immune response

1992 • 1,505 citations

STRUCTURE AND FUNCTION OF G PROTEIN-COUPLED RECEPTORS

1994 • 1,070 citations

Neonatal Tolerance Revisited: Turning on Newborn T Cells with Dendritic Cells

1996 • 770 citations

Induction of T _H 1 and T _H 2 Immunity in Neonatal Mice

1996 • 671 citations

Dendritic cells pulsed with protein antigens in vitro can prime antigen-specific, MHC-restricted T cells in situ.

1990 • 670 citations

The Thyrotropin Receptor and the Regulation of Thyrocyte Function and Growth*

1992 • 592 citations

Autoantibodies to the Thyrotropin Receptor

1988 • 568 citations

Molecular Cloning of the Thyrotropin Receptor

1989 • 529 citations

DNA-based immunization induces continuous secretion of hepatitis B surface antigen and high levels of circulating antibody

1993 • 401 citations

Direct Gene Transfer into Skeletal MuscleIn Vivo: Factors Affecting Efficiency of Transfer and Stability of Expression

1993 • 380 citations

Infection, Thyroid Disease, and Autoimmunity*

1993 • 336 citations

Resistance to Thyrotropin Caused by Mutations in the Thyrotropin-Receptor Gene

1995 • 333 citations

Familial Neonatal Transient Hypothyroidism Due to Maternal TSH-Binding Inhibitor Immunoglobulins

1980 • 314 citations

Direct Gene Transfer into Nonhuman Primate Myofibers In Vivo

1992 • 284 citations

Neonatal and early life immune responses to various forms of vaccine antigens qualitatively differ from adult responses: predominance of a Th2‐biased pattern which persists after adult boosting

1996 • 278 citations

Human Thyroid Adenyl Cyclase-Stimulating Activity in Immunoglobulin G of Patients with Graves' Disease

1976 • 240 citations

Induction of Graves-like disease in mice by immunization with fibroblasts transfected with the thyrotropin receptor and a class II molecule.

1996 • 226 citations

Modeling of the three-dimensional structure of proteins with the typical leucine-rich repeats

1995 • 225 citations

Diversity and Prevalence of Somatic Mutations in the Thyrotropin Receptor and G_sα Genes as a Cause of Toxic Thyroid Adenomas¹

1997 • 224 citations

Interpretation of binding curves obtained with high receptor concentrations: practical aid for computer analysis.

1995 • 182 citations

T Cell-Mediated Maintenance of Natural Self-Tolerance: its Breakdown as a Possible Cause of Various Autoimmune Diseases

1996 • 175 citations

Stable expression of the human TSH receptor in CHO cells and characterization of differentially expressing clones

1990 • 162 citations

Binding domains of stimulatory and inhibitory thyrotropin (TSH) receptor autoantibodies determined with chimeric TSH-lutropin/chorionic gonadotropin receptors.

1991 • 155 citations

Molecular mimicry: Can epitope mimicry induce autoimmune disease?

1997 • 143 citations

Constitutive activation of the TSH receptor by spontaneous mutations affecting the N‐terminal extracellular domain

1997 • 138 citations

Immunoglobulins from Graves' disease patients interact with different sites on TSH receptor/LH-CG receptor chimeras than either TSH or immunoglobulins from idiopathic myxedema patients

1991 • 135 citations

Induction of neonatal tolerance by plasmid DNA vaccination of mice.

1996 • 131 citations

Is there a ‘lock’ for all agonist ‘keys’ in 7TM receptors?

1996 • 130 citations

In vivo expression of a single viral DNA-binding protein generates systemic lupus erythematosus-related autoimmunity to double-stranded DNA and histones.

1995 • 110 citations

Molecular mimicry in the MHC: Hidden clues to autoimmunity?

1996 • 99 citations

Breaking self-tolerance in nonobese diabetic mice.

1996 • 88 citations

Identification of separate determinants on the thyrotropin receptor reactive with Graves' thyroid-stimulating antibodies and with thyroid-stimulating blocking antibodies in idiopathic myxedema: these determinants have no homologous sequence on gonadotropin receptors.

1992 • 82 citations

Expression of the Extracellular Domain of the Thyrotropin Receptor in the Baculovirus System Using a Promoter Active Earlier than the Polyhedrin Promoter

1995 • 81 citations

Specific activation of the thyrotropin receptor by trypsin

1996 • 79 citations

Use of the recombinant human thyrotropin receptor (TSH-R) expressed in mammalian cell lines to assay TSH-R autoantibodies

1990 • 74 citations

Identification of thyroid-stimulating antibody-specific interaction sites in the N-terminal region of the thyrotropin receptor.

1993 • 67 citations

Eleven amino acids (Lys-201 to Lys-211) and 9 amino acids (Gly-222 to Leu-230) in the human thyrotropin receptor are involved in ligand binding

1991 • 66 citations

Thyrotropin Receptor Autoantibodies in Serum Are Present at Much Lower Levels Than Thyroid Peroxidase Autoantibodies: Analysis by Flow Cytometry*

1997 • 65 citations

Monoclonal antibodies that recognize the native human thyrotropin receptor

1994 • 63 citations

Induction of Tsh Binding Inhibitory Immunoglobulins with the Extracellular Domain of Human Thyrotropin Receptor Produced Using Baculovirus Expression System

1993 • 63 citations

Recombinant thyrotropin receptor and the induction of autoimmune thyroid disease in BALB/c mice: a new animal model.

1994 • 59 citations

Defining the major antibody epitopes on the human thyrotropin receptor in immunized mice: evidence for intramolecular epitope spreading.

1995 • 55 citations

Current developments in G-protein-coupled receptors

1991 • 54 citations

Overexpression of the extracellular domain of the thyrotrophin receptor in bacteria; production of thyrotrophin-binding inhibiting immunoglobulins

1994 • 48 citations

Characterization of the extracellular region of the human thyrotrophin receptor expressed as a recombinant protein

1992 • 44 citations

Folding of the recombinant human thyrotropin (TSH) receptor extracellular domain: identification of folded monomeric and tetrameric complexes that bind TSH receptor autoantibodies.

1995 • 44 citations

Transfer of thyroiditis, with syngeneic spleen cells sensitized with the human thyrotropin receptor, to naive BALB/c and NOD mice.

1996 • 43 citations

The use of the amplifiable high-expression vector pEE14 to study the interactions of autoantibodies with recombinant human thyrotrophin receptor

1992 • 41 citations

Generation and characterization of monoclonal antibodies to the human thyrotropin (TSH) receptor: antibodies can bind to discrete conformational or linear epitopes and block TSH binding.

1995 • 40 citations

THYROID-STIMULATING ANTIBODIES IN GRAVES'S DISEASE AND THE EFFECT OF THYROTROPHIN-BINDING GLOBULINS ON THEIR DETERMINATION

1982 • 35 citations

The autoimmune response induced by immunising female mice with recombinant human thyrotropin receptor varies with the genetic background

1995 • 32 citations

Induction of Thyrotropin Receptor (TSH-R) Autoantibodies and Thyroiditis in Mice Immunized with the Recombinant TSH-R

1994 • 30 citations

Lipoprotein from Yersinia enterocolitica contains epitopes that cross-react with the human thyrotropin receptor.

1997 • 28 citations

Antigen presentation by dendritic cells provides optimal stimulation for the production of interleukin (IL) 2, IL 4 and interferon‐γ by allogeneic T cells

1991 • 27 citations

Yersinia enterocoliticaEnvelope Proteins that are Crossreactive with the Thyrotropin Receptor (TSHR) also have B-cell Mitogenic Activity

1996 • 25 citations

Molecular genetics of the thyrotropin receptor

1991 • 25 citations

Thyrotropin receptor-specific antibodies in BALB/cJ mice with experimental hyperthyroxinemia show a restricted binding specificity and belong to the immunoglobulin G1 subclass.

1995 • 22 citations

Patient with monoclonal gammopathy, thyrotoxicosis, pretibial myxedema and thyroid-associated ophthalmopathy; demonstration of direct binding of autoantibodies to the thyrotropin receptor

1996 • 22 citations

Functional Characteristics of a Variant Thyrotropin Receptor

1996 • 18 citations

The thyrotrophin receptor

1982 • 13 citations