2 Fatty Acid Desaturation

Phosphatidylcholine vesicles. Formation and physical characteristics

1969 • 1,522 citations

A simple method for the preparation of homogeneous phospholipid vesicles

1977 • 861 citations

Nutritional and hormonal factors influencing desaturation of essential fatty acids

1981 • 557 citations

Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid.

1976 • 552 citations

The Formation of Δ9-Unsaturated Fatty Acids

1960 • 503 citations

Purification and Properties of Rat Liver Microsomal Stearyl Coenzyme A Desaturase

1974 • 471 citations

A Function of Cytochrome b5 in Fatty Acid Desaturation by Rat Liver Microsomes

1971 • 389 citations

A Form of Reduced Nicotinamide Adenine Dinucleotide-Cytochrome b5 Reductase Containing Both the Catalytic Site and an Additional Hydrophobic Membrane-binding Segment

1973 • 344 citations

Binding of deoxycholate, Triton X-100, sodium dodecyl sulfate, and phosphatidylcholine vesicles to cytochrome b₅

1975 • 287 citations

The Binding of Cytochrome b5 to Liver Microsomes

1972 • 286 citations

Electron-transfer mechanism associated with fatty acid desaturation catalyzed by liver microsomes

1966 • 285 citations

Purification and characterization of the stearoyl-acyl carrier protein desaturase and the acyl-acyl carrier protein thioesterase from maturing seeds of safflower.

1982 • 271 citations

The desaturation step in the animal biosynthesis of polyunsaturated fatty acids

1971 • 228 citations

Cytochrome b5 reduction by NADPH-cytochrome P-450 reductase.

1979 • 199 citations

Metabolic Alterations of Fatty Acids

1974 • 196 citations

Studies to determine the role rates of chain elongation and desaturation play in regulating the unsaturated fatty acid composition of rat liver lipids

1975 • 176 citations

Reconstitution of Hepatic Microsomal Stearoyl-Coenzyme A Desaturase System from Solubilized Components*

1972 • 173 citations

The biosynthesis of unsaturated fatty acids and its control in mammalian liver.

1979 • 158 citations

Enzyme deletions and essential fatty acid metabolism in cultured cells.

1975 • 158 citations

A Requirement for Lipids by the Microsomal Stearyl Coenzyme A Desaturase

1969 • 158 citations

Hormonal regulation of stearoyl coenzyme A desaturase activity and lipogenesis during adipose conversion of 3T3-L1 cells.

1982 • 152 citations

The conversion of stearic to oleic acid by liver and yeast preparations

1962 • 148 citations

Lipid fluidity at the submacroscopic level: Determination by fluorescence polarization

1982 • 133 citations

Regulatory Function of Δ6 Desaturase — Key Enzyme of Polyunsaturated Fatty Acid Synthesis

1977 • 131 citations

Requirement for cytochrome b₅ in microsomal stearyl coenzyme A desaturation

1972 • 127 citations

Evidence for Random Distribution and Translational Movement of Cytochrome b5 in Endoplasmic Reticulum

1974 • 123 citations

The binding of cytochrome b5 to phospholipid vesicles and biological membranes. Effect of orientation on intermembrane transfer and digestion by carboxypeptidase Y.

1979 • 123 citations

Die enzymatische Dehydrierung der Stearinsäure zu Ölsäure

1959 • 120 citations

Requirement for Reduced Diphosphopyridine Nucleotide-Cytochrome b5 Reductase in Stearyl Coenzyme A Desaturation

1970 • 114 citations

Studies on the Biosynthesis of Microsomal Membrane Proteins

1982 • 114 citations

Modification and identification of cytochrome b5 carboxyl groups involved in protein-protein interaction with cytochrome b5 reductase.

1979 • 114 citations

Interrelationship between the dietary regulation of fatty acid synthesis and the fatty acyl‐CoA desaturases

1977 • 113 citations

Desaturation of isomeric trans-octadecenoic acids by rat liver microsomes

1980 • 107 citations

Apparent dependence of interactions between cytochrome b5 and cytochrome b5 reductase upon translational diffusion in dimyristoyl lecithin liposomes.

1975 • 96 citations

Requirement for three protein components in microsomal stearyl coenzyme A desaturation

1971 • 96 citations

Supply of polyenoic fatty acids to the mammalian brain: The ease of conversion of the short-chain essential fatty acids to their longer chain polyunsaturated metabolites in liver, brain, placenta and blood

1981 • 95 citations

Synthesis of rat liver microsomal cytochrome b5 by free ribosomes.

1980 • 93 citations

Characterization of the interaction of amphipathic cytochrome b5 with stearyl coenzyme A desaturase and NADPH:cytochrome P-450 reductase.

1980 • 90 citations

The interaction of NADH-cytochrome b5 reductase and cytochrome b5 bound to egg lecithin liposomes.

1975 • 88 citations

B-type cytochromes in plasma membranes isolated from rat liver, in comparison with those of endomembranes.

1979 • 88 citations

Synthesis of Fatty Acids in Animal Tissues

1964 • 80 citations

Purification and Properties of the Intact Form of NADH-Cytochrome b5 Reductase from Rabbit Liver Microsomes1

1975 • 78 citations

The primary structure of the nonpolar segment of bovine cytochrome b5

1978 • 77 citations

Structural and functional properties of the membrane binding segment of cytochrome b5.

1978 • 76 citations

Membrane‐bound phospholipid desaturases

1979 • 74 citations

Primary structure of the membranous segment of cytochrome b5.

1977 • 74 citations

Cytochrome b5 and cytochrome b5 reductase-phospholipid vesicles. Intervesicle protein transfer and oreintation factors in protein-protein interactions.

1977 • 74 citations

Covalent structure of the membranous segment of horse cytochrome b5. Chemical cleavage of the native hemoprotein

1977 • 71 citations

Regulation of rat hepatic stearoyl coenzyme A desaturase. The roles of insulin and carbohydrate.

1979 • 70 citations

Role of tyrosyl and arginyl residues in rat liver microsomal stearylcoenzyme A desaturase

1978 • 68 citations

Intramembrane position of the fluorescent tryptophanyl residue in membrane-bound cytochrome b5

1979 • 65 citations

The effects of estradiol on stearyl-CoA desaturase activity and microsomal membrane properties in rooster liver.

1979 • 65 citations

Orientation of the carboxyl and NH2 termini of the membrane-binding segment of cytochrome b5 on the same side of phospholipid bilayers.

1981 • 63 citations

Calorimetric and fluorescence characterization of interactions between cytochrome b5 and phosphatidylcholine bilayers

1983 • 63 citations

Purification and properties of hen liver microsomal terminal enzyme involved in stearoyl coenzyme A desaturation and its quantitation in neonatal chicks.

1979 • 61 citations

Chemical structure of rat liver cytochrome b5 Isolation of peptides by high-pressure liquid chromatography

1982 • 60 citations

Cytochrome b5-like Hemoprotein of Outer Mitochondrial Membrane; OM Cytochrome bI. Purification of OM Cytochrome b from Rat Liver Mitochondria and Comparison of Its Molecular Properties with Those of Cytochrome b5

1980 • 58 citations

The nutritional and metabolic impact of γ-linolenic acid (18:3ω6) on cats deprived of animal lipid

1978 • 57 citations

Hormonal regulation of the terminal enzyme of microsomal stearoyl coenzyme A desaturase in cultured avian liver explants. Role of insulin.

1979 • 55 citations

Mechanism of exchange of cytochrome b5 between phosphatidylcholine vesicles

1980 • 53 citations

Rate equations and simulation curves for enzymatic reactions which utilize lipids as substrates I. Interaction of enzymes with the monomers and micelles of soluble, amphiphilic lipids

1977 • 50 citations

Changes in fatty acid composition during preparative thin-layer chromatography

1963 • 46 citations

Self-association of cytochrome b5 in aqueous solution. Gel filtration and ultracentirfugational studies.

1976 • 46 citations

Involvement of cytochrome b₅ in the oxidative desaturation of linoleic acid to γ‐linolenic acid in rat liver microsomes

1977 • 45 citations

Mechanism of cytochrome b5 binding to phosphatidylcholine vesicles.

1979 • 44 citations

Reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase: location of the hydrophobic, membrane-binding region at the carboxyl-terminal end and the masked amino terminus

1978 • 44 citations

Study of lipid—protein interactions in membrane models: Intrinsic fluorescence of cytochrome b₅—phospholipid complexes

1975 • 43 citations

The rotational diffusion of cytochrome b5 in lipid bilayer membranes. Influence of the lipid physical state

1979 • 40 citations

Temperature-induced changes in fatty acid unsaturation of Tetrahymena membranes do not require induced fatty acid desaturase synthesis

1979 • 39 citations

The acyl-CoA desaturases of microsomes from rat liver and the Morris 7777 hepatoma

1979 • 39 citations

Desaturation of long-chain fatty acids by tissue preparations of the sheep, rat and chicken

1974 • 37 citations

Evidence for participation of cytochrome b5 in microsomal Δ-6 desaturation of fatty acids

1977 • 37 citations

Purification and Characterization of Cytochrome b5-like Hemoprotein Associated with Outer Mitochondrial Membrane of Rat Liver

1973 • 35 citations

Localization and biosynthesis of NADH-cytochrome b5 reductase, an integral membrane protein, in rat liver cells. II. Evidence that a single enzyme accounts for the activity in its various subcellular locations.

1980 • 35 citations

Desaturation of eicosa‐11,14‐dienoic acid in human testes

1979 • 34 citations

Essential fatty acid deficiency and evidence for arachidonate synthesis in the cat

1981 • 33 citations

Effect of cytochrome b5 on the size, density, and permeability of phosphatidylcholine vesicles

1975 • 31 citations

A new assay for fatty acid desaturation

1969 • 30 citations

A study of some of the factors involved in regulating the conversion of octadeca-8,11-dienoate to eicosa-4,7,10,13-tetraenoate in the rat

1971 • 30 citations

The Specificity of Mammalian Desaturases

1977 • 29 citations

The nonpolar peptide segment of cytochrome b5. Binding to phospholipid vesicles and identification of the fluorescent tryptophanyl residue.

1978 • 29 citations

Localization and Biosynthesis of NADH-Cytochrome b(5) reductase, an integral membrane protein, in rat liver cells. III. Evidence for the independent insertion and turnover of the enzyme in various subcellular compartments

1980 • 29 citations

Primary Structure of the Membrane-Binding Segment of Rabbit Cytochrome b5

1979 • 29 citations

Lipid products formed during desaturation of [1‐¹⁴C] stearyl CoA by hen liver microsomes

1974 • 27 citations

The substrate specificity of desaturases: The conversion of cis-12-octadecenoic acid into linoleic acid in different animal and plant species

1972 • 26 citations

Nature of Tryptic Attack on Cytochrome b5 and Further Evidence for the Two-Domain Structure of the Cytochrome Molecule1

1978 • 24 citations

The role of COOH-terminal anionic residues in binding cytochrome b5 to phospholipid vesicles and biological membranes.

1981 • 23 citations

Kinetics of the incorporation of cytochrome b₅, an integral membrane protein, into unilamellar dimyristoyllecithin liposomes

1978 • 23 citations

Inhibition of the microsomal stearoyl coenzyme a desaturation by divalent copper and its chelates

1980 • 20 citations

Regulatory mechanism of palmitoyl-CoA desaturase activity in thermal adaptation. Induction in non-growing Tetrahymena cells deprived of pre-existing desaturase

1980 • 20 citations

Asymmetric binding of cytochrome b5 to the membrane of human erythrocyte ghosts

1977 • 20 citations

Lipid binding properties of a factor necessary for linoleic acid desaturation

1979 • 19 citations

Topology of the delta 9 terminal desaturase in chicken liver microsomes and artificial micelles. Inhibition of the enzyme activity by the antibody and susceptibility of the enzyme to proteolysis.

1980 • 17 citations

Δ9- and Δ6-Desaturase Activities of the Ovine Placenta and Their Role in the Supply of Fatty Acids to the Fetus

1979 • 17 citations

Temperature-mediated hyperinduction of fatty acid desaturation in pre-existing and newly formed fatty acids synthesized endogenously in Bacillus megaterium

1980 • 15 citations

[31] Desaturation of long-chain fatty acids by animal liver

1975 • 13 citations

NADH- and NADPH-dependent desaturation of linoleic acid in the extracted microsomal fraction of rat liver, and related effects of catalase and hydrogen peroxide

1979 • 13 citations

Inhibition of the binding of cytochrome b5 to phosphatidylcholine vesicles by cholesterol

1979 • 12 citations

Solubilization of linoleoyl‐coenzyme a desaturase of rat liver microsomes

1979 • 12 citations

Mutant and immunochemical studies on the involvement of cytochrome b5 in fatty acid desaturation by yeast microsomes

1979 • 11 citations

Regulation of microsomal stearoyl-coenzyme A desaturase. Purification of a non-substrate-binding protein that stimulates activity

1979 • 10 citations

[31] Terminal enzyme of stearoyl-CoA desaturation from chicken liver

1981 • 9 citations

Preparation of a fluorescent derivative of cytochrome b5 and its interaction with phospholipids

1982 • 9 citations

The Interaction of Cytochrome b5 with Lipid Vesicles

1982 • 9 citations

Binding of cytochrome b5 to cholesterol-containing phosphatidylcholine vesicles

1978 • 8 citations

Protein Factor Involved in Fatty Acid Desaturation of Linoleic Acid

1977 • 6 citations

Possible Mechanism of Coupled NADPH Oxidase and P-450 Monooxygenase Action

1982 • 2 citations