Constant Denaturant Gel Electrophoresis (CDGE) in Mutation Screening

Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

1989 • 1,295 citations

DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory.

1983 • 895 citations

[30] Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis

1987 • 491 citations

Current methods of mutation detection

1993 • 263 citations

Recursion relation generation of probability profiles for specific‐sequence macromolecules with long‐range correlations

1974 • 225 citations

Germline mutations of the p53 tumor suppressor gene in children with osteosarcoma.

1994 • 197 citations

Constant denaturant gel electrophoresis as a rapid screening technique for p53 mutations.

1991 • 192 citations

Screening for germ line TP53 mutations in breast cancer patients.

1992 • 190 citations

Prognostic significance of TP53 alterations in breast carcinoma

1993 • 171 citations

Molecular Analysis of Phenylketonuria in Denmark: 99% of the Mutations Detected by Denaturing Gradient Gel Electrophoresis

1993 • 170 citations

Constant denaturant capillary electrophoresis (CDCE): a high resolution approach to mutational anaylsis

1994 • 169 citations

Mutations of the p53 gene do not occur in testis cancer.

1993 • 152 citations

Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection

1991 • 134 citations

Somatic p53 mutations in human breast carcinomas in an Icelandic population: a prognostic factor.

1993 • 122 citations

Detection of point mutations in the p53 gene: Comparison of single-strand conformation polymorphism, constant denaturant gel electrophoresis, and hydroxylamine and osmium tetroxide techniques

1993 • 99 citations

p53 mutations in lung tumors: relationship to putative susceptibility markers for cancer.

1994 • 96 citations

Evidence for a gene on 17p13.3, distal to TP53, as a target for allele loss in breast tumors without p53 mutations.

1994 • 91 citations

No germline TP53 mutations detected in familial and bilateral testicular cancer

1993 • 84 citations

Psoralen-modified oligonucleotide primers improve detection of mutations by denaturing gradient gel electrophoresis and provide an alternative to GC-clamping

1993 • 67 citations

Locations of frequently opening regions on natural DNAs and their relation to functional loci

1981 • 59 citations

P53 mutations in gastric carcinomas

1992 • 53 citations

Screening for TP53 mutations in osteosarcomas using constant denaturant gel electrophoresis (CDGE)

1993 • 44 citations

Denaturing gradient gel electrophoresis (DGGE) assay for K-ras and N-ras genes: detection of K-ras point mutations in human lung tumour DNA

1993 • 21 citations

Screening for mutations in human HPRT cDNA using the polymerase chain reaction (PCR) in combination with constant denaturant gel electrophoresis (CDGE)

1992 • 19 citations

Rapid characterization of HIV-1 sequence diversity using denaturing gradient gel electrophoresis and direct automated DNA sequencing of PCR products.

1993 • 15 citations

Analysis of exon 7 of the human phenylalanine hydroxylase gene: A mutation hot spot?

1992 • 15 citations

Detection of ras gene mutations in human lung cancer: comparison of two screening assays based on the polymerase chain reaction.

1992 • 12 citations

No alterations in exon 21 of the RBI gene in sarcomas and carcinomas of the breast, colon, and lung

1992 • 11 citations