Use of Pichia pastoris as a Model Eukaryotic System

THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY

1963 • 24,942 citations

Antibodies, a laboratory manual

1989 • 12,929 citations

Green Fluorescent Protein as a Marker for Gene Expression

1994 • 6,572 citations

Firefly luciferase gene: structure and expression in mammalian cells.

1987 • 3,088 citations

Mechanisms of intracellular protein transport

1994 • 2,280 citations

Wavelength mutations and posttranslational autoxidation of green fluorescent protein.

1994 • 1,773 citations

Peroxisomes (microbodies and related particles).

1966 • 1,635 citations

Understanding, improving and using green fluorescent proteins

1995 • 1,350 citations

Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling

1996 • 1,212 citations

A conserved tripeptide sorts proteins to peroxisomes.

1989 • 1,113 citations

BIOCHEMISTRY OF PEROXISOMES

1992 • 843 citations

A novel, cleavable peroxisomal targeting signal at the amino-terminus of the rat 3-ketoacyl-CoA thiolase.

1991 • 629 citations

Localization of nine glycolytic enzymes in a microbody‐like organelle in Trypanosoma brucei: The glycosome

1977 • 581 citations

A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast

1989 • 570 citations

[16] Isolation of subcellular organelles

1990 • 563 citations

Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase.

1987 • 481 citations

A rapid method for localized mutagenesis of yeast genes

1992 • 456 citations

Mutations in the PTS1 receptor gene, PXR1, define complementation group 2 of the peroxisome biogenesis disorders

1995 • 430 citations

A unified nomenclature for peroxisome biogenesis factors.

1996 • 413 citations

Protein Import into Peroxisomes and Biogenesis of the Organelle

1993 • 411 citations

PAS1, a yeast gene required for peroxisome biogenesis, encodes a member of a novel family of putative ATPases

1991 • 344 citations

An oligomeric protein is imported into peroxisomes in vivo.

1994 • 340 citations

Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae.

1989 • 326 citations

Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm

1967 • 321 citations

Red-Shifted Excitation Mutants of the Green Fluorescent Protein

1995 • 306 citations

Proliferation of microbodies in Saccharomyces cerevisiae

1987 • 302 citations

Peroxisomal protein import is conserved between yeast, plants, insects and mammals.

1990 • 296 citations

PAS7 encodes a novel yeast member of the WD-40 protein family essential for import of 3-oxoacyl-CoA thiolase, a PTS2-containing protein, into peroxisomes.

1994 • 295 citations

Disorders of peroxisome biogenesis

1995 • 289 citations

Amino-terminal presequence of the precursor of peroxisomal 3-ketoacyl-CoA thiolase is a cleavable signal peptide for peroxisomal targeting

1991 • 288 citations

Saccharomyces cerevisiae peroxisomal thiolase is imported as a dimer.

1994 • 277 citations

Import of stably folded proteins into peroxisomes.

1995 • 270 citations

Restoration by a 35K membrane protein of peroxisome assembly in a peroxisome-deficient mammalian cell mutant

1991 • 250 citations

Pex13p is an SH3 protein of the peroxisome membrane and a docking factor for the predominantly cytoplasmic PTs1 receptor.

1996 • 248 citations

The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome cells--the PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family [published erratum appears in J Cell Biol 1993 Sep;122(5):following 1143]

1993 • 246 citations

PAS10 is a tetratricopeptide-repeat protein that is essential for the import of most matrix proteins into peroxisomes of Saccharomyces cerevisiae.

1993 • 225 citations

Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product.

1988 • 220 citations

PAS3, a Saccharomyces cerevisiae gene encoding a peroxisomal integral membrane protein essential for peroxisome biogenesis.

1991 • 218 citations

Development of the yeast Pichia pastoris as a model organism for a genetic and molecular analysis of peroxisome assembly

1992 • 211 citations

How proteins penetrate peroxisomes

1995 • 198 citations

Acyl-CoA oxidase contains two targeting sequences each of which can mediate protein import into peroxisomes.

1988 • 196 citations

Firefly luciferase is targeted to peroxisomes in mammalian cells.

1987 • 189 citations

Human peroxisomal targeting signal-1 receptor restores peroxisomal protein import in cells from patients with fatal peroxisomal disorders.

1995 • 187 citations

The peroxisome biogenesis disorder group 4 gene, PXAAA1, encodes a cytoplasmic ATPase required for stability of the PTS1 receptor.

1996 • 184 citations

The import receptor for the peroxisomal targeting signal 2 (PTS2) in Saccharomyces cerevisiae is encoded by the PAS7 gene.

1996 • 181 citations

The Pichia pastoris peroxisomal protein PAS8p is the receptor for the C-terminal tripeptide peroxisomal targeting signal.

1995 • 178 citations

Identification and Characterization of the Putative Human Peroxisomal C-terminal Targeting Signal Import Receptor

1995 • 176 citations

The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop.

1996 • 170 citations

Visualization of the Peroxisomal Compartment in Living Mammalian Cells: Dynamic Behavior and Association with Microtubules

1997 • 170 citations

Evolutionary conservation of a microbody targeting signal that targets proteins to peroxisomes, glyoxysomes, and glycosomes.

1991 • 166 citations

The Hansenula polymorpha PER9 Gene Encodes a Peroxisomal Membrane Protein Essential for Peroxisome Assembly and Integrity

1996 • 162 citations

Mutagenesis of the amino targeting signal of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase reveals conserved amino acids required for import into peroxisomes in vivo.

1994 • 160 citations

The targeting and assembly of peroxisomal proteins: some old rules do not apply

1996 • 157 citations

The protein import receptor of mitochondria

1995 • 153 citations

Mutational analysis of the N-terminal topogenic signal of watermelon glyoxysomal malate dehydrogenase using the heterologous host Hansenula polymorpha.

1994 • 149 citations

Evidence for redox forms of the Aequorea green fluorescent protein

1994 • 146 citations

PEB1 (PAS7) in Saccharomyces cerevisiae encodes a hydrophilic, intra-peroxisomal protein that is a member of the WD repeat family and is essential for the import of thiolase into peroxisomes.

1995 • 140 citations

Two AAA Family Peroxins, PpPex1p and PpPex6p, Interact with Each Other in an ATP-Dependent Manner and Are Associated with Different Subcellular Membranous Structures Distinct from Peroxisomes

1998 • 140 citations

Antibodies directed against the peroxisomal targeting signal of firefly luciferase recognize multiple mammalian peroxisomal proteins.

1990 • 133 citations

Alcohol oxidase assembles post-translationally into the peroxisome of Candida boidinii.

1984 • 133 citations

The Hansenula polymorpha PER3 Gene Is Essential for the Import of PTS1 Proteins into the Peroxisomal Matrix

1995 • 130 citations

Isolation and Characterization of Pas2p, a Peroxisomal Membrane Protein Essential for Peroxisome Biogenesis in the Methylotrophic Yeast Pichia pastoris

1996 • 125 citations

Isolation of peroxisome assembly mutants from Saccharomyces cerevisiae with different morphologies using a novel positive selection procedure.

1992 • 123 citations

Characterization of the signal peptide at the amino terminus of the rat peroxisomal 3-ketoacyl-CoA thiolase precursor.

1994 • 121 citations

Pay32p of the yeast Yarrowia lipolytica is an intraperoxisomal component of the matrix protein translocation machinery.

1995 • 120 citations

Peroxisome‐deficient mutants of Hansenula polymorpha

1990 • 117 citations

Differential protein import deficiencies in human peroxisome assembly disorders.

1994 • 117 citations

Peroxisome assembly factor–2, a putative ATPase cloned by functional complementation on a peroxisome–deficient mammalian cell mutant

1995 • 113 citations

Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells.

1996 • 113 citations

An efficient screen for peroxisome-deficient mutants of Pichia pastoris

1992 • 113 citations

PER3, a Gene Required for Peroxisome Biogenesis in Pichia pastoris, Encodes a Peroxisomal Membrane Protein Involved in Protein Import

1995 • 113 citations

The Pichia pastoris PAS4 gene encodes a ubiquitin-conjugating enzyme required for peroxisome assembly.

1994 • 111 citations

PEX genes on the rise

1997 • 106 citations

Cloning and characterization of PAS5: a gene required for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris.

1993 • 102 citations

Mitochondrial protein import: Specific recognition and membrane translocation of preproteins

1993 • 102 citations

Peb1p (Pas7p) is an intraperoxisomal receptor for the NH2-terminal, type 2, peroxisomal targeting sequence of thiolase: Peb1p itself is targeted to peroxisomes by an NH2-terminal peptide.

1996 • 100 citations

Peroxisomal biogenesis: multiple pathways of protein import.

1994 • 97 citations

An efficient positive selection procedure for the isolation of peroxisomal import and peroxisome assembly mutants of Saccharomyces cerevisiae.

1993 • 92 citations

A Mobile PTS2 Receptor for Peroxisomal Protein Import in Pichia pastoris

1998 • 88 citations

An internal region of the peroxisomal membrane protein PMP47 is essential for sorting to peroxisomes

1994 • 84 citations

Sequence of the PAS8 gene, the product of which is essential for biogenesis of peroxisomes in Saccharomyces cerevisiae

1993 • 84 citations

Peroxisomes in the methylotrophic yeast Hansenula polymorpha do not necessarily derive from pre-existing organelles.

1993 • 78 citations

Rapid identification and characterization of peroxisomal assembly mutants in Yarrowia lipolytica

1993 • 78 citations

Characterization of a novel component of the peroxisomal protein import apparatus using fluorescent peroxisomal proteins.

1996 • 77 citations

Formation of the Peroxisome Lumen Is Abolished by Loss of Pichia pastoris Pas7p, a Zinc-Binding Integral Membrane Protein of the Peroxisome

1995 • 75 citations

Role of the PAS1 gene of Pichia pastoris in peroxisome biogenesis.

1994 • 75 citations

Biosynthesis of peroxisomal enzymes in the methylotrophic yeast Hansenula polymorpha

1983 • 72 citations

PAY4, a gene required for peroxisome assembly in the yeast Yarrowia lipolytica, encodes a novel member of a family of putative ATPases.

1994 • 69 citations

The Pichia pastoris PER6 Gene Product Is a Peroxisomal Integral Membrane Protein Essential for Peroxisome Biogenesis and Has Sequence Similarity to the Zellweger Syndrome Protein PAF-1

1996 • 67 citations

Characterization of the integral membrane polypeptides of rat liver peroxisomes isolated from untreated and clofibrate-treated rats

1991 • 61 citations

Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae.

1993 • 58 citations

The methylotrophic yeast Hansenula polymorpha contains an inducible import pathway for peroxisomal matrix proteins with an N-terminal targeting signal (PTS2 proteins).

1994 • 57 citations

The N‐terminus of amine oxidase of Hansenula polymorpha contains a peroxisomal targeting signal

1995 • 54 citations

Yeast peroxisomes

1979 • 50 citations

The hydrogenosomal enzyme hydrogenase from the anaerobic fungus Neocallimastix sp. L2 is recognized by antibodies, directed against the C-terminal microbody protein targeting signal SKL.

1993 • 45 citations

Peroxisome biogenesis and function in Hansenula polymorpha

1992 • 36 citations

The PAH2 gene is required for peroxisome assembly in the methylotrophic yeast Hansenula polymorpha and encodes a member of the tetratricopeptide repeat family of proteins

1995 • 36 citations

Induction, identification, and cell-free translation of mRNAs coding for peroxisomal proteins in Candida tropicalis.

1986 • 32 citations

Protein Import Deficiencies in Human Peroxisomal Disorders

1994 • 21 citations

Watermelon glyoxysomal malate dehydrogenase is sorted to peroxisomes of the methylotrophic yeast, Hansenula polymorpha

1993 • 18 citations

OCCURRENCE, PROLIFERATION AND METABOLIC FUNCTION OF YEAST MICROBODIES

1989 • 15 citations

Expression and Transport of Candida tropicalis Peroxisomal Acyl-coenzyme A Oxidase in the Yeast Candida maltosa

1989 • 14 citations