Spectrophotometric assay of thiols

Tissue sulfhydryl groups

1959 • 25,130 citations

Dithiothreitol, a New Protective Reagent for SH Groups^*

1964 • 1,933 citations

Spectrophotometric Study of the Reaction of Protein Sulfhydryl Groups with Organic Mercurials

1954 • 1,674 citations

Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid)—a reexamination

1979 • 997 citations

Determination of sulfhydryl groups with 2,2′- or 4,4′-dithiodipyridine

1967 • 978 citations

A scheme for the colorimetric determination of microgram amounts of thiols

1958 • 754 citations

X-ray Diffraction Study of Binding of 2,3-Diphosphoglycerate to Human Deoxyhaemoglobin

1972 • 731 citations

A methodology for analysis of tissue sulfhydryl components

1972 • 413 citations

Alterations in intracellular thiol homeostasis during the metabolism of menadione by isolated rat hepatocytes

1984 • 411 citations

Reactions of N-ethylmaleimide with peptides and amino acids

1964 • 396 citations

A modification of the nitroprusside method of analysis for glutathione.

1951 • 336 citations

Evidence for possible nonspecific reactions between N-ethylmaleimide and proteins

1967 • 243 citations

Determination of —SH Groups in Proteins

1962 • 183 citations

The reactivity of SH groups with a fluorogenic reagent

1970 • 177 citations

Electrostatic influence of local cysteine environments on disulfide exchange kinetics

1981 • 167 citations

Accurate measurement of oxidized glutathione content of human, rabbit, and rat red blood cells and tissues

1968 • 165 citations

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2′-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

1973 • 163 citations

THE DETERMINATION OF BLOOD GLUTATHIONE

1932 • 151 citations

The Mechanism of Inactivation of Glyceraldehyde 3-Phosphate Dehydrogenase by Tetrathionate, o-Iodosobenzoate, and Iodine Monochloride

1969 • 135 citations

Thiol reactivity of the nitrogenase Fe-protein from Azotobacter vinelandii.

1983 • 127 citations

Sulfhydryl and disulfide groups of platelet membranes. I. Determination of sulfhydryl groups

1973 • 118 citations

Specific covalent modification of thiols: Applications in the study of enzymes and other biomolecules

1979 • 118 citations

A Novel Physiological Role of Liver Glutathione as a Reservoir of L-Cysteine1

1977 • 117 citations

The effect of glutathione on protein sulphydryl groups in rat-liver homogenates

1962 • 108 citations

A spectrophotometric method for studying the rates of reaction of disulfides with protein thiol groups applied to bovine serum albumin

1980 • 103 citations

Mechanism of formation of disulfide-bonded multimers of plasma fibronectin in cell layers of cultured human fibroblasts.

1984 • 101 citations

Studies on Thiols. I. Oxidation of Thiol Groups by 2,6-Dichlorophenol Indophenol¹

1955 • 90 citations

Glutathione. A symposium

1955 • 88 citations

N-(4-Dimethylamino)-3,5-Dinitrophenyl)maleimide: A coloured sulfhydryl reagent

1960 • 87 citations

A new class of chromophoric organomercurials and their reactions with <scp>d</scp>-glyceraldehyde 3-phosphate dehydrogenase

1969 • 82 citations

Simultaneous inactivation of the catalytic activities of yeast glutathione reductase by N-alkylmaleimides

1981 • 79 citations

Complexes of serum albumin and cis-dichlorodiammineplatinum (II). The role of cysteine 34 as a nucleophilic entering group and evidence for reaction between bound platinum and a second macromolecule.

1983 • 74 citations

Determination of Sulfhydryl Groups in Certain Biological Substances

1954 • 59 citations

The reaction of protein thiol groups with some disulphides

1963 • 54 citations

Effect of sulfhydryl group modification on the activity of bovine ferrochelatase.

1984 • 54 citations

p-Mercuribenzoate as an Indicator of Conformation Change in Hemoglobin

1973 • 53 citations

Reversible uncoupling of photophosphorylation by a new bifunctional maleimide.

1979 • 44 citations

The reaction of 4,4′-bis-dimethylaminodiphenylcarbinol with the sulfhydryl group

1973 • 44 citations

Production of thiol groups and retention of calcium ions by cardiac mitochondria

1980 • 44 citations

A STUDY OF GLUTATHIONE

1930 • 43 citations

Reaction of the Catalytic Subunit of Escherichia coli Aspartate Transcarbamylase with Permanganate Ion, a Reactive Structural Analogue of Phosphate Ion

1971 • 42 citations

Reactions of <scp>l</scp>-ergothioneine and some other aminothiones with 2,2′- and 4,4′-dipyridyl disulphides and of <scp>l</scp>-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophiles

1974 • 41 citations

The reaction of carbonyl cyanide phenylhydrazones with thiols

1979 • 41 citations

Nitrogenase of Klebsiella pneumoniae: evidence for an adenosine triphosphate-induced association of the iron–sulphur protein

1973 • 41 citations

Colorimetric Assay for Reaction of Sulfhydryl Groups with Organic Mercurials

1957 • 40 citations

A SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF CYSTEINE AND RELATED COMPOUNDS

1956 • 34 citations

Studies on the effect of reagent and protein charges on reactivity of the β93 sulfhydryl group of human hemoglobin using selected mutations

1980 • 29 citations

Coupled oxidation of NADPH with thiols at neutral pH

1978 • 29 citations

Chromophoric labeling of yeast 3-phosphoglycerate kinase with an organomercurial

1974 • 29 citations

Spectrophotometric determination of disulfides, sulfinic acids, thio ethers, and thiols with the palladium (II) ion

1964 • 29 citations

Determination of the sulfhydryl content of thymus and liver using DPPH

1962 • 28 citations

Studies on (Na+ + K+)-activated ATPase. XLVI. Effect of cation-induced conformational changes on sulfhydryl group modification

1980 • 26 citations

Benzofuroxan as a thiol-specific reactivity probe. Kinetics of its reactions with papain, ficin, bromelain and low-molecular-weight thiols

1977 • 26 citations

Oxidation of reactive sulfhydryl groups of sarcoplasmic reticulum ATPase

1983 • 25 citations

Sulphydryl groups in photosynthetic energy conservation. V. Localization of the new disulfide bridges formed by o-iodosobenzoate in coupling factor of spinach chloroplasts

1979 • 23 citations

Inactivation of Salmonella phosphoribosylpyrophosphate synthetase by oxidation of a specific sulfhydryl group with potassium permanganate.

1975 • 22 citations

Reaction of cacodylic acid with organic thiols

1972 • 22 citations

The mechanism of dissociation of aspartate transcarbamoylase by p-mercuribenzoate.

1981 • 20 citations

Reactions of thiosulfate and sulfite ions with DTNB: Interference in sulfhydryl group analysis

1974 • 20 citations

Characterization of a new sulfhydryl group reagent: 6,6′-Diselenobis-(3-Nitrobenzoic acid), a selenium analog of Ellman's reagent

1981 • 20 citations

Ionization of a nitrophenol-containing reporter group at the active site of papain

1974 • 20 citations

The thiol group of the L-arabinose-binding protein. Chromophoric labeling and chemical identification of the sugar-binding site.

1979 • 20 citations

The essential sulfhydryl group of ornithine transcarbamylases. Reaction with anionic, aromatic disulfides and properties of its cyano derivative.

1980 • 19 citations

A chromatographic method of analysis for thiols

1957 • 19 citations

Estimation of thiol content of basic proteins in acid solution by Saville's method

1969 • 19 citations

Oxidation of Biological Thiols:Glutathione-Indophenol Interactions^*

1964 • 16 citations

A rapid assay for sulphite reductase

1964 • 16 citations

A kinetic method for the study of solvent environments of thiol groups in proteins involving the use of a pair of isomeric reactivity probes and a differential solvent effect. Investigation of the active centre of ficin by using 2,2'- and 4,4'- dipyridyl disulphides as reactivity probes

1980 • 16 citations

Anomalous reaction of 4-chloro-7-nitrobenzofurazan with thiol compounds

1979 • 16 citations

Colorimetric measurement of cuprous ion formation to detect certain reducing agents as illustrated with cysteine, glutathione, and serum albumin

1958 • 16 citations

Direct spectrophotometric estimation of ferrocyanide and its possible uses in sulfhydril oxidation studies

1969 • 16 citations

Functional sulfhydryl groups of ferredoxin-NADP+ oxidoreductase

1982 • 14 citations

Photometric Determination of Aconitic Acid with Potassium Permanganate

1952 • 14 citations

Structural changes induced in glycogen phosphorylase by the binding of glucose and caffeine

1980 • 13 citations

Determination of acid-soluble thiols and disulfides in transplanted animal tumors

1965 • 13 citations

A possible involvement of sulfhydryl groups in the conversion of lysine monooxygenase to an oxidase.

1975 • 12 citations

Thiol group determination in proteins by flameless atomic absorption spectrometry of mercury

1975 • 11 citations

Kinetics of thymidylate synthase inhibition by disulfides

1980 • 11 citations

Selective enzyme inactivation by benzoylacrylic acid derivatives

1982 • 11 citations

Synthesis and properties of 5-membered heterocyclic disulfides: Application for enzyme modification

1984 • 11 citations

The Nitroprusside Method for the Determination of Reduced Glutathione

1964 • 11 citations

4,4′-Dinitrodiphenyldisulfides of different charge type as probes for the electrostatic environment of sulfhydryl groups

1975 • 10 citations

4,4′-Bis(dimethylamino)diphenylcarbinol, a sensitive spray reagent for the detection of thiols and carboxylic acids

1976 • 10 citations

The modification of the catalytic chain sulfhydryl group of aspartate transcarbamylase with mercurinitrophenols.

1979 • 10 citations

Studies on latent derivatives of aminoethanethiols as potentially selective cytoprotectants. I. A spectrophotometric assay for aminoethanethiols in biologic media.

1967 • 10 citations

Modification of the Thiol Residues of Pyridine Nucleotide Transhydrogenase from Azotobacter vinelandii. Activity Modulation by the Divalent Thiol Reagent p-Aminophenylarsenoxide

1981 • 9 citations

A diagonal procedure for isolating sulfhydryl peptides alkylated with N-ethylmaleimide

1980 • 8 citations

A thiol-labelling reagent and reactivity probe containing electrophilic mercury and a chromophoric leaving group

1979 • 8 citations

Sulfhydryl reagents inhibit electron transport in Photosystem II of spinach chloroplasts

1982 • 7 citations

Preparation of photoreactive derivatives of glutathione and [9-(2-mercaptotryptophan)]corticotropin by selective modification of the sulfhydryl group

1981 • 7 citations

Structure-activity relationships in tryptophanyl transfer ribonucleic acid synthetase from beef pancreas. Influence of the alkylation of the sulfhydryl groups on the dimer-monomer equilibrium.

1975 • 6 citations

The oxidation of β-lactoglobulin in sodium dodecyl sulfate solutions

1968 • 6 citations

Unmasking of an essential thiol during function of the membrane-bound enzyme II of the phosphoenolpyruvate β-glucoside phosphotransferase system of Escherichia coli

1979 • 5 citations

Cleavage of the SS bond in 5,5′-dithiobis-(2-nitrobenzoic acid) in the presence of a cationic detergent An approach to the cleavage of the SS bond in bovine plasma albumin

1977 • 4 citations

Reversible dissociation of cortisol-transcortin complex by sodium para-chloromercuribenzoate

1983 • 2 citations