Hydrogenases in Lithoautotrophic Bacteria

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

1983 • 7,336 citations

Rapid procedure for detection and isolation of large and small plasmids

1981 • 2,840 citations

Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

1980 • 2,669 citations

The Journal of General Microbiology

1985 • 2,446 citations

Biology of anaerobic microorganisms

1989 • 2,196 citations

A broad host range mobilization system for in vivo genetic engineering. Transposon mutagenesis in Gram negative bacteria

1983 • 1,115 citations

Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants

1982 • 857 citations

Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals

1985 • 798 citations

Plasmids related to the broad host range vector, pRK290, useful for gene cloning and for monitoring gene expression

1985 • 791 citations

Identification and Characterization of Large Plasmids in Rhizobium meliloti using Agarose Gel Electrophoresis

1979 • 574 citations

Wide host range cloning vectors: A cosmid clone bank of an Agrobacterium Ti plasmid

1982 • 568 citations

Properties of six pesticide degradation plasmids isolated from Alcaligenes paradoxus and Alcaligenes eutrophus

1981 • 533 citations

Nucleic Acid Homologies in the Genus Pseudomonas

1973 • 531 citations

Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.

1978 • 514 citations

Nucleotide sequence and expression of the selenocysteine-containing polypeptide of formate dehydrogenase (formate-hydrogen-lyase-linked) from Escherichia coli.

1986 • 475 citations

Hydrogenase

1980 • 439 citations

Nitrogen fixation hundred years after

1989 • 419 citations

Hydrogenosome, a Cytoplasmic Organelle of the Anaerobic Flagellate Tritrichomonas foetus, and Its Role in Pyruvate Metabolism

1973 • 415 citations

Methanogens and the diversity of archaebacteria

1987 • 407 citations

Rearrangement of the Species and Genera of the Phototrophic "Purple Nonsulfur Bacteria"

1984 • 353 citations

Products of nitrogen regulatory genes ntrA and ntrC of enteric bacteria activate glnA transcription in vitro: evidence that the ntrA product is a sigma factor.

1985 • 352 citations

Transcription of glnA by purified Escherichia coli components: core RNA polymerase and the products of glnF, glnG, and glnL.

1985 • 335 citations

Biological Formation of Molecular Hydrogen

1965 • 320 citations

Nitrogen fixation hundred years after

1988 • 308 citations

Mechanisms of mRNA decay in bacteria: a perspective

1988 • 290 citations

Physiology and Biochemistry of Aerobic Hydrogen-Oxidizing Bacteria

1981 • 285 citations

Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.

1986 • 283 citations

CO₂fixation in acetogenic bacteria: Variations on a theme

1986 • 283 citations

Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligenes eutrophus

1981 • 270 citations

The three classes of hydrogenases from sulfate-reducing bacteria of the genusDesulfovibrio

1988 • 266 citations

Molecular and cellular regulation of autotrophic carbon dioxide fixation in microorganisms

1988 • 265 citations

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

1987 • 241 citations

Nickel utilization by microorganisms

1987 • 233 citations

The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties

1979 • 221 citations

Methanogens and the diversity of archaebacteria.

1987 • 213 citations

Nucleotide sequence of the gene encoding the hydrogenase from Desulfovibrio vulgaris (Hildenborough)

1985 • 190 citations

Genetic Engineering for Nitrogen Fixation

1977 • 184 citations

Cloning of plasmid genes encoding resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus CH34

1987 • 183 citations

Regulation of nitrogen fixation genes

1984 • 182 citations

The CO2 assimilation via the reductive tricarboxylic acid cycle in an obligately autotrophic, aerobic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus

1985 • 179 citations

Growth of the photosynthetic bacterium Rhodopseudomonas capsulata chemoautotrophically in darkness with H2 as the energy source

1979 • 178 citations

Hydrogenobacter thermophilus gen. nov., sp. nov., an Extremely Thermophilic, Aerobic, Hydrogen-Oxidizing Bacterium

1984 • 164 citations

Essential and non-essential domains in theBradyrhizobium japonicumNifA protein: identification of indispensable cysteine residues potentially involved in redox reactivity and/or metal binding

1988 • 163 citations

Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus

1981 • 161 citations

Nickel in the environment

1981 • 152 citations

Hydrogenobacter thermophilus gen. nov., sp. nov., an Extremely Thermophilic, Aerobic, Hydrogen-Oxidizing Bacterium

1984 • 147 citations

8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization

1987 • 137 citations

The iron-sulphur centres of soluble hydrogenase from Alcaligenes eutrophus

1979 • 133 citations

A hydrogenase-linked gene in Methanobacterium thermoautotrophicum strain delta H encodes a polyferredoxin.

1989 • 129 citations

Nickel utilization by microorganisms.

1987 • 128 citations

Formation of Enzymes of Autotrophic Metabolism During Heterotrophic Growth of Alcaligenes eutrophus

1981 • 124 citations

Physiology, Biochemistry, and Genetics of the Uptake Hydrogenase in Rhizobia

1987 • 124 citations

Genetic and physiological characterization of new Escherichia coli mutants impaired in hydrogenase activity

1986 • 124 citations

Pleiotropic hydrogenase mutants of Escherichia coli K12: growth in the presence of nickel can restore hydrogenase activity

1986 • 122 citations

Nucleotide sequence of the genetic loci encoding subunits of Bradyrhizobium japonicum uptake hydrogenase.

1988 • 117 citations

Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus

1982 • 116 citations

Nickel enzymes

1987 • 116 citations

Involvement of the ntrA gene product in the anaerobic metabolism of Escherichia coli

1987 • 116 citations

Expression of hydrogenase activity in free-living Rhizobium japonicum

1978 • 115 citations

Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus

1988 • 114 citations

Contribution of hydrogen production by biological nitrogen fixation to the global hydrogen budget

1980 • 114 citations

Ferredoxin requirement for electron transport from the carbon monoxide dehydrogenase complex to a membrane-bound hydrogenase in acetate-grown Methanosarcina thermophila.

1988 • 111 citations

Nickel-Dependent Chemolithotrophic Growth of Two Hydrogenomonas Strains

1965 • 107 citations

Content and localization of FMN, Fe‐S clusters and nickel in the NAD‐linked hydrogenase of Nocardia opaca 1b

1984 • 107 citations

Molecular and cellular regulation of autotrophic carbon dioxide fixation in microorganisms.

1988 • 106 citations

Co-transfer of determinants for hydrogenase activity and nodulation ability in Rhizobium leguminosarum

1980 • 104 citations

Production of superoxide radicals by soluble hydrogenase from Alcaligenes eutrophus H16

1981 • 104 citations

Decomposition of atmospheric hydrogen by soil microorganisms and soil enzymes

1981 • 99 citations

Effect of molecular hydrogen and carbon dioxide on chemo-organotrophic growth of Acetobacterium woodii and Clostridium aceticum

1981 • 98 citations

Cloning and sequencing of the genes encoding the large and small subunits of the periplasmic (NiFeSe) hydrogenase of Desulfovibrio baculatus

1987 • 96 citations

Paramagnetic centers in the nickel-containing, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum

1983 • 93 citations

Oxidation of thiosulfate byParacoccus denitrificansand other hydrogen bacteria

1981 • 91 citations

Nickel transport in Methanobacterium bryantii

1982 • 90 citations

Biogeochemistry and Ecophysiology of Atmospheric CO and H2

1988 • 88 citations

Alcaligenes eutrophus hydrogenase genes (Hox)

1984 • 88 citations

Depression of hydrogenase during limitation of electron donors and derepression of ribulosebisphosphate carboxylase during carbon limitation of Alcaligenes eutrophus

1982 • 86 citations

Purification and properties of the particulate hydrogenase from the bacteroids of soybean root nodules

1979 • 83 citations

Purification and characterization of an 8‐hydroxy‐5‐deazaflavin‐reducing hydrogenase from the archaebacterium Methanococcus voltae

1987 • 82 citations

Effect of nickel on activity and subunit composition of purified hydrogenase from Nocardia opaca 1b

1984 • 79 citations

Proton translocation coupled to the oxidation of carbon monoxide to CO₂ and H₂ in Methanosarcina barkeri

1989 • 76 citations

ESR properties of membrane-bound hydrogenases from aerobic hydrogen bacteria

1983 • 75 citations

Construction of a Rhizobium japonicum gene bank and use in isolation of a hydrogen uptake gene

1983 • 74 citations

The nucleotide sequence of the sigma factor gene ntrA (rpoN) of Azotobacter vinelandii: Analysis of conserved sequences in NtrA proteins

1987 • 74 citations

Metabolism of carbon monoxide by Rhodopseudomonas gelatinosa: cell growth and properties of the oxidation system

1983 • 74 citations

Transport and accumulation of nickel ions in the cyanobacterium Anabaena cylindrica

1986 • 73 citations

Cloning, Characterization, and Sequencing of the Genes Encoding the Large and Small Subunits of the Periplasmic [NiFe]hydrogenase ofDesulfovibrio gigas

1987 • 72 citations

Revertible hydrogen uptake-deficient mutants of Rhizobium japonicum

1981 • 72 citations

Physical evidence for plasmids in autotrophic, especially hydrogen-oxidizing bacteria

1982 • 71 citations

Characterization of Rhizobium japonicum hydrogen uptake genes

1984 • 67 citations

Chemolithoautotrophic growth of bacteria able to grow under N₂-fixing conditions

1981 • 67 citations

The mechanism of hydrogen photoproduction by several algae

1972 • 67 citations

The inability of hydrogen bacteria to utilize atmospheric hydrogen is due to threshold and affinity for hydrogen

1983 • 63 citations

An rpoN-like gene of Alcaligenes eutrophus and Pseudomonas facilis controls expression of diverse metabolic pathways, including hydrogen oxidation

1989 • 63 citations

Genetic determinants of a nickel-specific transport system are part of the plasmid-encoded hydrogenase gene cluster in Alcaligenes eutrophus

1989 • 63 citations

Cloning and characterization of hydrogen uptake genes from Rhizobium leguminosarum

1987 • 61 citations

Homology and distribution of CO dehydrogenase structural genes in carboxydotrophic bacteria

1989 • 61 citations

Rhizobium japonicum hydrogenase: Purification to homogeneity from soybean nodules, and molecular characterization

1985 • 60 citations

Purification of the F₄₂₀‐reducing hydrogenase from Methanosarcina barkeri (strain Fusaro)

1989 • 59 citations

Temperature control of nitrogen fixation in Klebsiella pneumoniae

1979 • 58 citations

Putative signal peptide on the small subunit of the periplasmic hydrogenase from Desulfovibrio vulgaris

1986 • 58 citations

Hydrogenase from Acetobacterium woodii

1984 • 58 citations

Application of hydrogenase for photoinduced hydrogen evolution

1986 • 57 citations

Expression of hydrogenase in Alcaligenes spp. is altered by interspecific plasmid exchange

1984 • 56 citations

Intra- and interspecies transfer and expression of Rhizobium japonicum hydrogen uptake genes and autotrophic growth capability

1985 • 55 citations

The membrane-bound hydrogenase of Rhodopseudomonas capsulata is inducible and contains nickel

1983 • 55 citations

Posttranscriptional control of Klebsiella pneumoniae nif mRNA stability by the nifL product

1986 • 55 citations

Hydrogenases of phototrophic microorganisms

1986 • 54 citations

Nickel transport in Alcaligenes eutrophus

1987 • 53 citations

Purification, characterization and redox properties of hydrogenase from Methanosarcina barkeri (DSM 800)

1984 • 53 citations

European journal of biochemistry

1995 • 53 citations

Evidence for an unusual mechanism of membrane translocation of the periplasmic hydrogenase ofDesulfovibrio vulgaris(Hildenborough), as derived from expression inEscherichia coli

1988 • 51 citations

Chromosomal and plasmid locations for phosphoribulokinase genes in Alcaligenes eutrophus

1985 • 50 citations

Plasmids required for utilization of molecular hydrogen by Alcaligenes eutrophus

1981 • 49 citations

Molecular cloning of structural and regulatory hydrogenase (hox) genes of Alcaligenes eutrophus H16

1986 • 48 citations

Construction and use of a gene bank of Alcaligenes eutrophus in the analysis of ribulose bisphosphate carboxylase genes

1984 • 47 citations

Denitrification by Alcaligenes eutrophus is plasmid dependent

1985 • 47 citations

N2-Fixation by chemoautotrophic hydrogen bacteria

1974 • 47 citations

Cloning of DNA fragments carrying hydrogenase genes of Rhodopseudomonas capsulata

1986 • 47 citations

Genes of lithoautotrophic metabolism are clustered on the megaplasmid pHG1 in Alcaligenes eutrophus

1987 • 46 citations

Biochemistry, Regulation, and Genetics of Hydrogen Oxidation in Rhizobium

1985 • 46 citations

Chemoautotrophic growth of Rhodopseudomonas species with hydrogen and chemotrophic utilization of methanol and formate

1979 • 46 citations

Immunological and molecular evidence for a membrane-bound, dimeric hydrogenase in Rhodopseudomonas capsulata

1987 • 45 citations

Nitrogenase ? hydrogenase relationships in Rhizobium japonicum

1984 • 45 citations

A NEW SPECIES OF HYDROGEN-UTILIZING BACTERIUM

1979 • 44 citations

Molecular and immunological comparison of membrane-bound, H2-oxidizing hydrogenases of Bradyrhizobium japonicum, Alcaligenes eutrophus, Alcaligenes latus, and Azotobacter vinelandii

1985 • 43 citations

Nickel uptake in Bradyrhizobium japonicum

1987 • 42 citations

8-hydroxy-5-deazaflavin-reducing hydrogenase from methanobacterium thermoautotrophicum: 2. Kinetic and hydrogen-transfer studies. Derivation of a steady-state rate equation for deazaflavin-reducing hydrogenase

1987 • 42 citations

Isolation and characterization of megaplasmid DNA from lithoautotrophic bacteria

1984 • 42 citations

Biochemical and genetic analysis of hydrogen metabolism in Escherichia coli: the hydB gene

1988 • 39 citations

Growth of thermophilic, obligatorily chemolithoautotrophic hydrogen-oxidizing bacteria related toHydrogenobacterwith thiosulfate and elemental sulfur as electron and energy source

1986 • 39 citations

Isolation of genes (nif/hup cosmids) involved in hydrogenase and nitrogenase activities in Rhizobium japonicum

1985 • 38 citations

Energieabh�ngige 63Ni-Aufnahme bei Alcaligenes eutrophus Stamm H 1 und H 16

1977 • 38 citations

Purification and properties of the membrane-bound hydrogenase from N2-fixing Alcaligenes latus

1983 • 38 citations

Regulation of hydrogenase formation is temperature sensitive and plasmid coded in Alcaligenes eutrophus

1983 • 35 citations

Involvement of megaplasmids in heterotrophic derepression of the carbon-dioxide assimilating enzyme system in Alcaligenes spp.

1984 • 35 citations

Regulation of hydrogenase biosynthesis by nickel in Bradyrhizobium japonicum

1986 • 35 citations

Localization of the F420-reducing hydrogenase in Methanococcus voltae cells by immuno-gold technique

1988 • 33 citations

Plasmids in carboxydotrophic bacteria: physical and restriction analysis

1988 • 32 citations

Properties of the particulate enzyme F₄₂₀-reducing hydrogenase isolated from Methanospirillum hungatei

1987 • 32 citations

Physiological characterization of the hydrogen bacterium Aquaspirillum autotrophicum

1978 • 32 citations

Inhibition of hydrogenase synthesis by DNA gyrase inhibitors in Bradyrhizobium japonicum

1987 • 29 citations

Organization of genes necessary for growth of the hydrogen-methanol autotroph Xanthobacter sp. strain H4-14 on hydrogen and carbon dioxide

1985 • 29 citations

Genetic engineering in agriculture: hydrogen uptake (hup) genes

1980 • 29 citations

No Correlation Exists between the Conjugative Transfer of the Autotrophic Character and That of Plasmids in Nocardia opaca Strains

1986 • 28 citations

Energy Coupling Efficiency of Symbiotic Nitrogen Fixation

1977 • 28 citations

Expression of uptake hydrogenase and hydrogen oxidation during heterotrophic growth of Bradyrhizobium japonicum

1987 • 28 citations

Hydrogen Autotrophy as a Transferable Genetic Character of Nocardia opaca 1b

1981 • 27 citations

Isolation and immunological characterization of the four non-identical subunits of the soluble NAD-linked hydrogenase from Alcaligenes eutrophus H16

1986 • 27 citations

Evidence for plasmid coding of the ability to utilize hydrogen gas by Pseudomonas facilis

1977 • 27 citations

Characterization of a native subunit of the NAD-linked hydrogenase isolated from a mutant of Alcaligenes eutrophus H16

1986 • 27 citations

Identification and isolation of genes essential for H2 oxidation in Rhodobacter capsulatus

1989 • 26 citations

Hydrogenase of purple bacteria: properties and regulation of synthesis

1984 • 26 citations

The regulation of hydrogenase formation as a differentiating character of strains of Paracoccus denitrificans

1980 • 25 citations

Regulation by molecular oxygen and organic substrates of hydrogenase synthesis in Alcaligenes eutrophus

1984 • 24 citations

Nickel and iron incorporation into soluble hydrogenase of Alcaligenes eutrophus

1984 • 23 citations

In vivo inactivation of soluble hydrogenase of Alcaligenes eutrophus

1981 • 23 citations

Nif- Hup- mutants of Rhizobium japonicum

1983 • 22 citations

Locations of the hydrogenases of Methanobacterium formicicum after subcellular fractionation of cell extract

1987 • 22 citations

Cloning and expression of Bradyrhizobium japonicum uptake hydrogenase structural genes in Escherichia coli.

1986 • 20 citations

Regulation of H2 oxidation activity and hydrogenase protein levels by H2, O2, and carbon substrates in Alcaligenes latus

1987 • 20 citations

Mutagenesis of a Rhizobium Plasmid Carrying Hydrogenase Determinants

1985 • 20 citations

Pleotropic mutants from Alcaligenes eutrophus defective in the metabolism of hydrogen, nitrate, urea, and fumarate

1988 • 19 citations

Randomly induced Escherichia coli K-12 Tn5 insertion mutants defective in hydrogenase activity

1989 • 19 citations

Isolation of Genes Required for Hydrogenase Synthesis in Escherichia coli

1987 • 18 citations

Nickel uptake in Rhodopseudomonas capsulata

1987 • 18 citations

Competitive inhibition of the membrane-bound hydrogenase of Alcaligenes eutrophus by molecular oxygen

1980 • 18 citations

Immunocytochemical localization of the soluble NAD-dependent hydrogenase in cells ofAlcaligenes eutrophus

1986 • 18 citations

Selenium increases hydrogenase expression in autotrophically cultured Bradyrhizobium japonicum and is a constituent of the purified enzyme

1988 • 17 citations

The hydrogenase of a thermophilic hydrogen-oxidizing bacterium

1983 • 17 citations

Conjugational Gene Transfer in Xanthobacter autotrophicus GZ29

1980 • 17 citations

Hydrodynamic Parameters of the Detergent‐Solubilised Hydrogenase from Paracoccus denitrificans

1979 • 17 citations

The membrane‐bound hydrogenase from Paracoccus denitrificans

1989 • 16 citations

Conjugal transfer of hydrogen-oxidizing ability of Alcaligeneshydrogenophilus to Pseudomonasoxalaticus

1986 • 16 citations

Hydrogenase and ribulose-1, 5-bisphosphate carboxylase activities of Alcaligenes eutrophus ATCC 17706 associated with an indigenous plasmid

1983 • 16 citations

Cloning and Sequencing of the Gene Encoding the Large and Small Subunits of the Periplasmic (NiFeSe) Hydrogenase of Desulfovibrio baculatus

1988 • 15 citations

Further evidence that two unique subunits are essential for expression of hydrogenase activity in Rhizobium japonicum

1985 • 15 citations

Oxygen tolerance of strictly aerobic hydrogen-oxidizing bacteria

1982 • 15 citations

Relationship between carbon monoxide dehydrogenase and a small plasmid inPseudomonas carboxydovorans

1985 • 14 citations

Mutants of Pseudomonas facilis Defective in Lithoautotrophy

1986 • 14 citations

Purification and properties of a protein linked to the soluble hydrogenase of hydrogen-oxidizing bacteria

1987 • 13 citations

The formation of an oxygen-binding flavohemoprotein in Alcaligenes eutrophus is plasmid-determined

1989 • 13 citations

Sulfide-repressed, membrane-bound hydrogenase in the thermophilic facultative phototroph,Chloroflexus aurantiacus

1985 • 13 citations

Transfer and Expression of Lithoautotrophy and Denitrification in a Host Lacking These Metabolic Activities

1988 • 12 citations

Effect of redox potential on the activation of the NAD-dependent hydrogenase from Alcaligenes eutrophus Z1

1989 • 11 citations

Isolation of hydrogen-oxidation gene from Alcaligeneshydrogenophilus and its expression in Pseudomonasoxalaticus

1986 • 11 citations

A Defective Generalized Transducing Bacteriophage in Xanthobacter autotrophicus GZ29

1979 • 9 citations

Genetic transfer of lithoautotrophy mediated by a plasmid-cointegrate from Pseudomonas facilis

1989 • 8 citations

Enzymes of the autotrophic pathway in mating partners and transconjugants of Nocardia opaca 1 b and Rhodococcus erythropolis

1986 • 8 citations

Investigation of the H2-oxidizing activities of Alcaligenes eutrophus H16 membranes with artificial electron acceptors, respiratory inhibitors and redox-spectroscopic procedures

1986 • 7 citations

Autotrophic growth and hydrogenase activity ofPseudomonas saccharophilastrains

1983 • 7 citations

Autotrophic growth and hydrogenase activity of Pseudomonas saccharophila strains

1983 • 7 citations

Electron microscopic localization of hydrogenase genes on the megaplasmid pHG 1 in Alcaligenes eutrophus

1986 • 7 citations

Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase (hup)of Rhodobacter capsulatus

1989 • 5 citations

Identification of new peptides synthesized under the hydrogenase control system of Alcaligenes eutrophus

1987 • 4 citations

Effect of Hydrogen, Sucrose and Oxygen on Uptake Hydrogenase in Nitrogen-fixing and Ammonium-grown Pseudomonas saccharophila ATCC 15946

1988 • 4 citations

N-Hydroxides, aminotriazole, and cyanide activate ribulosebisphosphate carboxylase formation inAlcaligenes eutrophus

1984 • 3 citations

KEY ENZYMES OF AUTOTROPHIC METABOLISM IN HYDROGEN-OXIDIZING BACTERIA: REGULATION OF THEIR FORMATION

1985 • 1 citations