[7] The third, fourth, and fifth components of human complement: Isolation and biochemical properties

Immunochemical quantitation of antigens by single radial immunodiffusion

1965 • 9,369 citations

Plasminogen: Purification from Human Plasma by Affinity Chromatography

1970 • 2,160 citations

A simplified method for cyanogen bromide activation of agarose for affinity chromatography

1974 • 1,951 citations

Polyacrylamide Gel Electrophoresis of Viral Proteins

1971 • 1,477 citations

The isolation of IgG from mammalian sera with the aid of caprylic acid

1969 • 874 citations

Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution.

1977 • 685 citations

Reductive alkylation of amino groups in proteins

1968 • 678 citations

Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum

1966 • 651 citations

Determination of the Tryptophan Content of Proteins by Ion Exchange Chromatography of Alkaline Hydrolysates

1972 • 592 citations

Third component of human complement: purification from plasma and physicochemical characterization

1976 • 523 citations

Large scale isolation of functionally active components of the human complement system.

1981 • 446 citations

ISOLATION OF A FRAGMENT (C3a) OF THE THIRD COMPONENT OF HUMAN COMPLEMENT CONTAINING ANAPHYLATOXIN AND CHEMOTACTIC ACTIVITY AND DESCRIPTION OF AN ANAPHYLATOXIN INACTIVATOR OF HUMAN SERUM

1969 • 430 citations

Molecular Biology and Chemistry of the Alternative Pathway of Complement

1980 • 409 citations

Relation of putative thioester bond in C3 to activation of the alternative pathway and the binding of C3b to biological targets of complement.

1980 • 328 citations

C3 PROACTIVATOR CONVERTASE AND ITS MODE OF ACTION

1972 • 326 citations

Two Anticomplementary Factors in Cobra Venom: Hemolysis of Guinea Pig Erythrocytes by One of Them

1969 • 301 citations

Evidence for an Ester Linkage between the Labile Binding Site of C3b and Receptive Surfaces

1979 • 297 citations

Human C4-binding protein. II. Role in proteolysis of C4b by C3b-inactivator.

1978 • 285 citations

REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS

1970 • 275 citations

Activation of the Complement System by Antibody-Antigen Complexes: The Classical Pathway

1979 • 258 citations

Covalent chromatography. Preparation of fully active papain from dried papaya latex

1973 • 256 citations

FORMATION AND FUNCTIONAL SIGNIFICANCE OF A MOLECULAR COMPLEX DERIVED FROM THE SECOND AND THE FOURTH COMPONENT OF HUMAN COMPLEMENT

1967 • 244 citations

Immune Hemolysis: A Simplified Method for the Preparation of EaC′4 with Guinea Pig or with Human Complement

1967 • 222 citations

Tritium labeling of proteins to high specific radioactivity by reduction methylation.

1980 • 206 citations

Alternative pathway of complement: recruitment of precursor properdin by the labile C3/C5 convertase and the potentiation of the pathway.

1976 • 204 citations

THE REACTION MECHANISM OF HUMAN C5 IN IMMUNE HEMOLYSIS

1970 • 196 citations

Hydroxylapatite Chromatography of Protein-Sodium Dodecyl Sulfate Complexes

1972 • 194 citations

FOURTH COMPONENT OF HUMAN COMPLEMENT: DESCRIPTION OF A THREE POLYPEPTIDE CHAIN STRUCTURE

1974 • 192 citations

A New One-Step Method for the Functional Assay of the Fourth Component (C4) of Human and Guinea Pig Complement

1974 • 188 citations

The physiological breakdown of the third component of human complement

1980 • 187 citations

Partial characterization of human C5a anaphylatoxin. I. Chemical description of the carbohydrate and polypeptide prtions of human C5a.

1976 • 185 citations

The stoichiometric measurement of the serum inhibition of the first component of complement by the inhibition of immune hemolysis.

1968 • 178 citations

Quantitative determination of γ-carboxyglutamic acid in proteins

1977 • 177 citations

MOLECULAR ANALYSIS OF THE MEMBRANE ATTACK MECHANISM OF COMPLEMENT

1972 • 171 citations

The binding of human complement component C4 to antibody-antigen aggregates

1980 • 162 citations

Modulation of C3b Hemolytic Activity by a Plasma Protein Distinct from C3b Inactivator

1976 • 161 citations

Mechanism of action of the C3b inactivator: Requiremen for a high molecular weight cofactor (C3b-C4bINA cofactor) and production of a new C3b derivative (C3b′)

1977 • 152 citations

Third component of human complement: structural requirements for its function

1980 • 138 citations

Third component of human complement: appearance of a sulfhydryl group following chemical or enzymic inactivation

1980 • 137 citations

Autolytic fragmentation of complement components C3 and C4 under denaturing conditions, a property shared with α 2-macroglobulin

1981 • 128 citations

Action of the C3b-Inactivator on Cell-Bound C3b

1979 • 126 citations

A new function of the activated third component of complement: binding to C5, an essential step for C5 activation.

1978 • 126 citations

Heat-induced fragmentation of human alpha 2-macroglobulin.

1979 • 125 citations

Purification and structural analysis of the fourth component of human complement

1977 • 123 citations

Release of endogenous C3b inactivator from lymphocytes in response to triggering membrane receptors for beta 1H globulin.

1980 • 123 citations

C3e: An Acidic Fragment of Human C3 with Leukocytosis-Inducing Activity

1979 • 115 citations

The isolation and structure of C4, the fourth component of human complement

1977 • 114 citations

C3 Requirements for Formation of Alternative Pathway C5 Convertase

1976 • 110 citations

Fifth component of human complement: purification from plasma and polypeptide chain structure

1979 • 110 citations

Human C3 and C5: Subunit Structure and Modifications by Trypsin and C4̄2̄-C4̄2̄3̄

1975 • 107 citations

Limited degradation of the third component (C3) of human complement by human leukocyte elastase (HLE): partial characterization of C3 fragments

1977 • 106 citations

The specificity of purified porcine pancreatic elastase

1969 • 105 citations

Fourth component of human complement: studies of an amine-sensitive site comprised of a thiol component

1981 • 104 citations

Partial Characterization of Human C5a Anaphylatoxin

1976 • 104 citations

Purification and partial characterization of human and porcine C3a anaphylatoxin.

1975 • 101 citations

Third component of human complement: structural analysis of the polypeptide chains of C3 and C3b

1979 • 97 citations

Interaction between the labile binding sites of the fourth (C4) and fifth (C5) human complement proteins and erythrocyte cell membranes.

1980 • 97 citations

The temperature-sensitive bond in human alpha 2-macroglobulin is the alkylamine-reactive site.

1980 • 96 citations

Biosynthesis of pro-C3, a precursor of the third component of complement.

1977 • 96 citations

The separation of nine components and two inactivators of components of component in human serum

1970 • 90 citations

Rapid and effective removal of sodium dodecyl sulfate from proteins

1971 • 85 citations

Biosynthesis and Post-Synthetic Modification of a Precursor (PRO-C5) of the Fifth Component of Mouse Complement (C5)

1979 • 79 citations

Fragment of the third component of human complement with anaphylatoxin activity

1971 • 77 citations

Studies of the molecular mechanisms of C3b inactivation and a simplified assay of beta 1H and the C3b inactivator (C3bINA).

1979 • 75 citations

Amino acid sequence of the tryptic peptide containing the alkylamine-reactive site from human alpha 2-macroglobulin. Identification of gamma-glutamylmethylamide.

1980 • 74 citations

Methylamine reaction and denaturation-dependent fragmentation of complement component 3. Comparison with alpha2-macroglobulin.

1980 • 71 citations

The mechanism of action of the C3b inactivator (conglutinogen- activating factor) on its naturally occurring substrate, the major fragment of the third component of complement (C3b)

1975 • 70 citations

On the Mechanism of Cell Membrane Damage by Complement: Evidence on Insertion of Polypeptide Chains from C8 and C9 into the Lipid Bilayer of Erythrocytes

1977 • 69 citations

Studies of Complement Complex C5b,6 Eluted from—EAC-6: Reaction of C5b,6 with EAC4b,3b and Evidence on the Role of C2a and C3b in the Activation of C5

1974 • 68 citations

Effect of methylamine on the structure and function of the fourth component of human complement, C4.

1980 • 65 citations

Cleavage of the fourth component of human complement (C4) by C1 esterase: Isolation and characterization of the low molecular weight product

1970 • 63 citations

The assembly of early components of complement on antibody-antigen aggregates and on antibody-coated erythrocytes

1978 • 56 citations

Cleavage of the fourth component of human complement (C4) by activated activated Cls

1970 • 48 citations

Enzymic activity of the second component of complement

1975 • 46 citations

Novel cleavage products of the third component of human complement

1980 • 39 citations

A New Activity of Complement Component C3: Cell-Bound C3b Potentiates Lysis of Erythrocytes by C5b,6 and Terminal Components

1976 • 35 citations

Polyacrylamide Gel Electrophoresis (PAGE) of Reduced and Dissociated C3 and C5: Studies of the Polypeptide Chain (PPC) Subunits and Their Modifications by Trypsin (TRY) and C4̄2̄-4̄2̄3̄.

1973 • 32 citations

Biosynthesis of a Single Chain PRO-C5 by Normal Mouse Liver mRNA: Analysis of the Molecular Basis of C5 Deficiency in AKR/J Mice

1979 • 18 citations

Amino acid sequence around the proposed thiolester bond of human complement component C4 and comparison with the corresponding sequences from C3 and α2-macroglobulin

1981 • 16 citations

Biochemistry and Biological Reactions of Complement Proteins

1979 • 14 citations

Preservation of C3, C5, and C5a Functional Activities by a New Radiolabeling Method; Demonstration of C5 Products in Complement-Activated Serum

1979 • 14 citations