The Phenotype of the Minichromosome Maintenance Mutant mcm3 Is Characteristic of Mutants Defective in DNA Replication

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

Methods in Yeast Genetics

1988 • 4,905 citations

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

1983 • 2,609 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

Transformation of yeast.

1978 • 2,267 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

The distribution of the numbers of mutants in bacterial populations

1949 • 1,471 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

The molecular biology of the yeast saccharomyces

1982 • 1,165 citations

The localization of replication origins on ARS plasmids in S. cerevisiae

1987 • 1,051 citations

A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: Application to sequencing the corn mitochondrial 18 S rDNA

1985 • 1,051 citations

The making of strand-specific M13 probes

1982 • 652 citations

Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene

1980 • 511 citations

Cloned Viral Protein Vaccine for Foot-and-Mouth Disease: Responses in Cattle and Swine

1981 • 504 citations

Eucaryotic DNA: Organization of the genome for replication

1978 • 476 citations

Assembly of the mitochondrial membrane system. CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b.

1985 • 467 citations

[2] Production of antisera with small doses of immunogen: multiple intradermal injections

1981 • 442 citations

The in vivo replication origin of the yeast 2μm plasmid

1987 • 437 citations

MUTANTS OF S. CEREVISIAE DEFECTIVE IN THE MAINTENANCE OF MINICHROMOSOMES

1984 • 419 citations

Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts

1983 • 396 citations

Genetic map of Saccharomyces cerevisiae

1980 • 394 citations

ALTERED FIDELITY OF MITOTIC CHROMOSOME TRANSMISSION IN CELL CYCLE MUTANTS OF S. CEREVISIAE

1985 • 390 citations

A repressor (MATα2 product) and its operator control expression of a set of cell type specific genes in yeast

1985 • 368 citations

Chapter 16 The Use of Fluorescent DNA-Binding Agent for Detecting and Separating Yeast Mitochondrial DNA

1975 • 364 citations

Localization and Sequence Analysis of Yeast Origins of DNA Replication

1983 • 364 citations

Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication.

1987 • 355 citations

Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: Studies in Xenopus

1990 • 354 citations

Saccharomyces cerevisiae protein involved in plasmid maintenance is necessary for mating of MATα cells

1988 • 323 citations

Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.

1986 • 306 citations

Three Additional Genes Required for Deoxyribonucleic Acid Synthesis in Saccharomyces cerevisiae

1973 • 278 citations

High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene

1979 • 269 citations

Extensive unwinding of the plasmid template during staged enzymatic initiation of DNA replication from the origin of the Escherichia coli chromosome

1986 • 249 citations

Transformation in Escherichia coli: cryogenic preservation of competent cells

1977 • 242 citations

A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes.

1989 • 239 citations

Identification of silencer binding proteins from yeast: possible roles in SIR control and DNA replication

1987 • 238 citations

Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer.

1988 • 234 citations

Structural requirements for the function of a yeast chromosomal replicator

1984 • 231 citations

The ease of DNA unwinding as a determinant of initiation at yeast replication origins

1988 • 193 citations

Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications

1977 • 192 citations

Similarity Between the Transcriptional Silencer Binding Proteins ABF1 and RAP1

1989 • 180 citations

Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction.

1986 • 179 citations

Close association of a DNA replication origin and anARSelement on chromosome III of the yeast,Saccharomyces cerevisiae

1988 • 175 citations

Roles of two DNA-binding factors in replication, segregation and transcriptional repression mediated by a yeast silencer.

1988 • 174 citations

DNA polymerase III, a second essential DNA polymerase, is encoded by the S. cerevisiae CDC2 gene

1989 • 154 citations

Expression of calf prochymosin in Saccharomyces cerevisiae

1984 • 115 citations

A DNA replication enhancer in Saccharomyces cerevisiae.

1990 • 100 citations

An extensive deletion causing overproduction of yeast iso-2-cytochrome c

1981 • 93 citations

MOLECULAR AND GENETIC RECOMBINATION OF BACTERIOPHAGE T4

1975 • 86 citations

Identification of a sequence responsible for periodic synthesis of yeast histone 2A mRNA.

1982 • 83 citations

Fiber autoradiography of replicating yeast DNA

1975 • 74 citations

Specific interaction between a Saccharomyces cerevisiae protein and a DNA element associated with certain autonomously replicating sequences.

1988 • 73 citations

A mutant that affects the function of autonomously replicating sequences in yeast

1986 • 65 citations

Enhanced mitotic recombination in a ligase-defective mutant of the yeast Saccharomyces cerevisiae.

1979 • 53 citations

Construction of telocentric chromosomes in Saccharomyces cerevisiae.

1985 • 47 citations

Structure of DNA in DNA replication mutants of yeast

1974 • 43 citations