Prolyl Hydroxylase

The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

1969 • 20,498 citations

Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins

1971 • 1,396 citations

The thermal transition of a non-hydroxylated form of collagen. Evidence for a role for hydroxyproline in stabilizing the triple-helix of collagen

1973 • 563 citations

The effect of ascorbic acid on collagen polypeptide synthesis and proline hydroxylation during the growth of cultured fibroblasts

1972 • 377 citations

The Protein Component of Primary Cell Walls

1966 • 376 citations

A rapid assay for collagen proline hydroxylase

1966 • 333 citations

Cofactor and substrate requirements of collagen proline hydroxylase

1967 • 300 citations

Evidence for the Linkage of a Disaccharide to Hydroxylysine in Tropocollagen

1966 • 298 citations

Characterization and Quantitative Determination of the Hydroxylysine-linked Carbohydrate Units of Several Collagens

1969 • 289 citations

Synthesis and extrusion of collagen by freshly isolated cells from chick embryo tendon

1971 • 280 citations

SOME ASPECTS OF THE METABOLISM OF HYDROXYPROLINE, STUDIED WITH THE AID OF ISOTOPIC NITROGEN

1949 • 277 citations

Isolation and characterization of C1q, a subcomponent of the first component of complement, from human and rabbit sera

1972 • 272 citations

The Amino Acid Composition of Some Fish Collagens: The Relation between Composition and Structure

1960 • 240 citations

ASCORBIC ACID AND THE FORMATION OF COLLAGEN

1953 • 238 citations

The Nature of the Collagen Synthesized by Cultured Human Fibroblasts

1971 • 238 citations

Procollagen Peptidase: An Enzyme Excising the Coordination Peptides of Procollagen

1971 • 232 citations

Hydroxyproline-O-glycosidic Linkage of the Plant Cell Wall Glycoprotein Extensin

1967 • 224 citations

Hydroxyproline in Primary Cell Walls of Higher Plants

1960 • 215 citations

Hydroxyproline stabilizes the triple helix of chick tendon collagen

1973 • 201 citations

Evidence for Procollagen, a Biosynthetic Precursor of Collagen

1971 • 193 citations

Collagen Proline Hydroxylase in Wound Healing, Granuloma Formation, Scurvy, and Growth

1967 • 185 citations

ENZYMATIC HYDROXYLATION OF PROLINE IN MICROSOMAL POLYPEPTIDE LEADING TO FORMATION OF COLLAGEN

1965 • 184 citations

Hydroxylation of Proline and the Intracellular Accumulation of a Polypeptide Precursor of Collagen

1966 • 180 citations

Conversion of Proline to Collagen Hydroxyproline in a Cell-free System from Chick Embryo

1963 • 180 citations

A method for immediate visualization of proteins in acrylamide gels and its use for preparation of antibodies to enzymes

1969 • 178 citations

THE METABOLISM OF l(-)-PROLINE STUDIED WITH THE AID OF DEUTERIUM AND ISOTOPIC NITROGEN

1944 • 176 citations

ENZYMATIC HYDROXYLATION OF PROLINE AND LYSINE IN PROTOCOLLAGEN

1967 • 171 citations

Collagen and Cell Protein Synthesis by an Established Mammalian Fibroblast Line

1964 • 169 citations

Affinity Column Purification of Protocollagen Proline Hydroxylase from Chick Embryos and Further Characterization of the Enzyme

1973 • 168 citations

Hydroxylation of Proline in Synthetic Polypeptides with Purified Protocollagen Hydroxylase

1967 • 167 citations

Function of Ascorbic Acid in the Conversion of Proline to Collagen Hydroxyproline

1962 • 158 citations

Purification and properties of collagen proline hydroxylase from newborn rat skin

1970 • 152 citations

Comparative Sequence Studies of Rat Skin and Tendon Collagen. I. Evidence for Incomplete Hydroxylation of Individual Prolyl Residues in the Normal Proteins^*

1967 • 149 citations

Requirements for α-ketoglutarate, ferrous ion and ascorbate by collagen proline hydroxylase

1966 • 148 citations

Formation of collagen hydroxyproline in vitro

1959 • 148 citations

The Amino Acid Sequence of Peptides from the Cross-Linking Region of Rat Skin Collagen^*

1967 • 146 citations

A method for the simultaneous measurement of the radioactivity of proline-C14 and hydroxyproline-C14 in biological materials

1962 • 139 citations

Biosynthesis of abnormal collagens with amino acid analogues

1969 • 134 citations

Cofactor Requirements of γ-Butyrobetaine Hydroxylase from Rat Liver

1970 • 133 citations

Biological Hydroxylations and Ascorbic Acid with Special Regard to Collagen Metabolism

1972 • 130 citations

SYNTHESIS OF HYDROXYPROLINE IN VITRO BY THE HYDROXYLATION OF PROLINE IN A PRECURSOR OF COLLAGEN

1965 • 130 citations

Hydroxylation of proline residues in collagen nascent chains

1970 • 129 citations

Decarboxylation of alpha-ketoglutarate coupled to collagen proline hydroxylase.

1968 • 127 citations

A Hydroxyproline-containing, Collagen-like Protein in Plasma and a Procedure for Its Assay

1964 • 124 citations

Purification of carbon-14-labeled protocollagen and its hydroxylation by prolyl-hydroxylase

1973 • 120 citations

Ascaris Cuticle Collagen: on the Disulfide Cross-Linkages and the Molecular Properties of the Subunits^*

1967 • 120 citations

Influence of chelating agents on the biosynthesis of collagen

1965 • 119 citations

Chemical studies on Clq; A modulator of immunoglobulin biology

1971 • 115 citations

Incorporation of 18O from air into hydroxyproline by chick embryo

1962 • 113 citations

Progress in bioorganic chemistry

1971 • 112 citations

Oxygen Fixation into Hydroxyproline of Plant Cell Wall Protein

1963 • 109 citations

Possible Participation of Superoxide Anion in the Intestinal Tryptophan 2,3-Dioxygenase Reaction

1971 • 107 citations

Synthesis and Secretion of Hydroxyproline Containing Macromolecules in Carrots. I. Kinetic Analysis

1969 • 105 citations

Collagen Synthesis: Localization of Prolyl Hydroxylase in Tendon Cells Detected with Ferritin-Labeled Antibodies

1973 • 105 citations

Intracellular Collagen and Protocollagen from Embryonic Tendon Cells

1973 • 103 citations

Protocollagen Proline Hydroxylase Activity in the Skin of Normal Human Subjects and of Patients with Scleroderma

1969 • 103 citations

The thermal denaturation of collagen in solution and its structural implications

1959 • 103 citations

A Kinetic Study of Collagen Biosynthesis

1972 • 102 citations

Kinetics of Collagen Synthesis by Established Mammalian Cell Lines

1963 • 101 citations

A Comparative Study of Glycopeptides Derived from Selected Vertebrate Collagens

1970 • 101 citations

The Relation of Ascorbic Acid to the Conversion of Proline to Hydroxyproline in the Synthesis of Collagen in the Carrageenan Granuloma

1959 • 100 citations

Peptide Chain Synthesis of Human Hemoglobins A and A2

1966 • 96 citations

Collagen chain formation and peptidyl proline hydroxylation in monolayer tissue cultures of L-929 fibroblasts

1969 • 95 citations

Isolation and partial characterization of highly purified protocollagen proline hydroxylase

1970 • 93 citations

Cofactor requirements for the enzymatic hydroxylation of lysine in a polypeptide precursor of collagen

1967 • 93 citations

Incorporation of cis-Hydroxyproline into Protocollagen and Collagen

1971 • 91 citations

Partial purification and characterization of protocollagen lysine hydroxylase from chick embryos

1972 • 90 citations

Effect of Lactate on Collagen Proline Hydroxylase Activity in Cultured L-929 Fibroblasts

1970 • 87 citations

Synthesis of collagen by mammalian cell lines of fibroblastic and nonfibroblastic origin.

1965 • 87 citations

Activation of Prolyl Hydroxylase in L-929 Fibroblasts by Ascorbic Acid

1973 • 86 citations

Proposed Mechanism for the Biological Assembly of Collagen Triple Helix

1971 • 85 citations

Collagen synthesis on polyribosomes of cultured mammalian fibroblasts

1967 • 84 citations

Evidence for the Nature of the Precursor That Is Hydroxylated during the Biosynthesis of Collagen Hydroxyproline

1965 • 84 citations

Protocollagen lysine hydroxylase. Hydroxylation of synthetic peptides and the stoichiometric decarboxylation of α-ketoglutarate

1972 • 83 citations

Increased protocollagen hydroxylase activity in the livers of rats with hepatic fibrosis

1967 • 81 citations

Procollagen: Conversion of the Precursor to Collagen by a Neutral Protease

1972 • 80 citations

Isolation, Configuration, and Synthesis of Natural cis- and trans-3-Hydroxyprolines

1963 • 80 citations

Oxygen-18 studies on the conversion of proline to collagen hydroxyproline

1963 • 79 citations

Proteins and Plant Cell Walls. Proline to Hydroxyproline in Tobacco Suspension Cultures

1964 • 78 citations

3-Hydroxyproline, a New Amino Acid of Collagen

1962 • 77 citations

Incorporation of 3,4-Dehydroproline into Protocollagen and Collagen

1970 • 77 citations

THE SOURCE AND STATE OF THE HYDROXYLYSINE OF COLLAGEN

1955 • 75 citations

Synthetic polypeptides as substrates and inhibitors of collagen proline hydroxylase

1968 • 75 citations

INTRACELLULAR ACCUMULATION OF PROTOCOLLAGEN AND EXTRUSION OF COLLAGEN BY EMBRYONIC CARTILAGE CELLS

1968 • 75 citations

The effects of selected nitrogen compounds on the growth of plant tissue cultures

1958 • 73 citations

Hydorxyproline biosynthesis in plant cells Peptidyl proline hydroxylase from carrot disks

1971 • 73 citations

Studies on protocollagen: Identification of a precursor of proto α1

1971 • 68 citations

The Preparation of cis- and trans-4-H³-L-Prolines and Their Use in Studying the Mechanism of Enzymatic Hydroxylation in Chick Embryos

1964 • 67 citations

Inhibition of proline and lysine hydroxylation prevents normal extrusion of collagen by 3T6 fibroblasts in culture

1971 • 67 citations

Partial characterization of protocollagen from embryonic cartilage

1967 • 67 citations

Collagen polysomes: Site of hydroxylation of proline residues

1971 • 66 citations

PROLINE-HYDROXYLASE ACTIVITY IN HUMAN BLOOD

1970 • 65 citations

Conversion of Proline to Collagen Hydroxyproline^*

1966 • 65 citations

Synthesis and Secretion of Hydroxyproline-containing Macromolecules in Carrots

1970 • 63 citations

Precursors of the Hydroxyproline and Ketoproline in Actinomycin

1962 • 63 citations

Kinetic patterns of protocollagen hydroxylase and further studies on the polypeptide substrate

1968 • 62 citations

Biosynthesis of abnormal collagens with amino acid analogues

1969 • 61 citations

The Nitrogenous Constituents of Plants with Special Reference to Chromatographic Methods

1950 • 61 citations

The Use of C¹⁴-Proline by Growing Cell: Its Conversion to Protein and Hydroxyproline

1959 • 61 citations

The synthesis of underhydroxylated collagen by 3T6 mouse fibroblasts in culture

1972 • 60 citations

Prolyl hydroxylase and an immunologically related protein in mammalian tissues

1974 • 60 citations

14C-Proline and Hydroxyproline in the Protein Metabolism of Plants: An Episode in the Relation of Metabolism to Cell Growth and Morphogenesis

1958 • 59 citations

Formation of Enzyme-Substrate Complexes with Protocollagen Proline Hydroxylase and Large Polypeptide Substrates

1969 • 59 citations

Immunological Evidence for an Inactive Precursor of Collagen Proline Hydroxylase in Cultured Fibroblasts

1971 • 58 citations

Incorporation of Hydroxyproline into Tissue Proteins by Chick Embryos

1959 • 56 citations

Conversion of the amino acid sequence Gly-Pro-Pro in protein to Gly-Pro-Hyp by collagen proline hydroxylase

1967 • 56 citations

The role of hydroxylation in the secretion of collagen by mouse fibroblasts in culture

1971 • 55 citations

Hydroxylation of (X-Pro-Gly)n by protocollagen proline hydroxylase Effect of chain length, helical conformation and amino acid sequence in the substrate

1972 • 54 citations

Elevation of Aortic Proline Hydroxylase: A Biochemical Defect in Experimental Arteriosclerosis

1970 • 54 citations

The isolation of allohydroxy-<scp>l</scp>-proline from sandal (Santalum album L.)

1954 • 54 citations

Simultaneous incorporation of 18O into succinate and hydroxyproline catalyzed by collagen proline hydroxylase

1971 • 53 citations

Conversion of Proline to Hydroxyproline and Its Incorporation into Collagen

1961 • 53 citations

Mechanism of .alpha.-ketoglutarate oxidation in coupled enzymic oxygenations

1969 • 52 citations

Oxygen-18 studies on the conversion of proline to hydroxyproline

1962 • 52 citations

Partial Hydroxylation of Certain Lysines In Collagen

1968 • 51 citations

γ-Butyrobetaine hydroxylase from Pseudomonas sp AK 1

1970 • 50 citations

Collagen biosynthesis the formation of hydroxyproline and soluble-ribonucleic acid complexes of proline and hydroxyproline by chick embryo in vivo and by a subcellular chick-embryo system

1963 • 50 citations

Control of the citric acid cycle by glyoxylate. Mechanism of the inhibition by oxalomalate and γ-hydroxy-α-oxoglutarate

1967 • 49 citations

Further study on the activation of collagen proline hydroxylase in cultures of L-929 fibroblasts

1970 • 49 citations

Factors which stimulate collagen proline hydroxylase

1967 • 48 citations

Glycylhydroxyprolyl Sequences in Earthworm Cuticle Collagen: Glycylhydroxyprolylserine

1970 • 47 citations

Cofactor requirements of thymine 7-hydroxylase

1967 • 47 citations

The substrate recognition site of collagen proline hydroxylase: The hydroxylation of -X-Pro-Gly- sequences in bradykinin analogs and other peptides

1971 • 46 citations

Protocollagen proline hydroxylase: Molecular weight, subunits and isoelectric point

1970 • 46 citations

Rapid assay for lysyl-protocollagen hydroxylase activity

1972 • 46 citations

Augmentation of collagen synthesis by ascorbic acid in vitro

1961 • 45 citations

The sulfhydryl nature of collagen proline hydroxylase

1969 • 44 citations

Relation between Collagen Synthesis and Collagen Proline Hydroxylase Activity in Mammalian Cells

1969 • 44 citations

Distribution and Metabolism of Protein-Bound Hydroxyproline in an Elongating Tissue, the Avena Coleoptile

1968 • 43 citations

A rapid assay for 14C-labeled hydroxylysine in collagen and related materials

1969 • 43 citations

Collagen Synthesis by Human Fibroblast Strains.

1964 • 43 citations

α-KETOGLUTARATE-COUPLED DIOXYGENASES

1974 • 42 citations

Protocollagen Proline Hydroxylase from Ascaris lumbricoides

1969 • 42 citations

THE AMINO ACIDS OF ALFALFA AS REVEALED BY PAPER CHROMATOGRAPHY WITH SPECIAL REFERENCE TO COMPOUNDS LABELLED WITH S³⁵

1951 • 42 citations

α-Ketoglutarate and hydroxylation of γ-butyrobetaine

1968 • 41 citations

BIOSYNTHESIS OF COLLAGEN

1967 • 41 citations

Partial purification and characterization of peptidyl proline hydroxylase precursor from mouse fibroblasts

1972 • 40 citations

Growth and macromolecular synthesis in the 3T6 mouse fibroblast: I. general description and the role of ascorbic acid

1970 • 40 citations

Oligopeptides with the Sequences Ala-Pro-Gly and Gly-Pro-Gly as Substrates or Inhibitors for Protocollagen Proline Hydroxylase

1969 • 40 citations

Effect of Polymer Size on the Inhibition of Protocollagen Proline Hydroxylase by Polyproline II

1969 • 39 citations

The size of the polypeptide precursor of collagen hydroxyproline.

1966 • 39 citations

Proline and Hydroxyproline in Leaves

1952 • 39 citations

Preparation, Resolution and Optical Stability of 3,4-Dehydroproline and 3,4-Dehydroprolinamide

1962 • 39 citations

Collagen Synthesis in Human Skin Fibroblasts: Effects of Ascorbate, α-Ketoglutarate and Ferrous Ion on Proline Hydroxylation

1972 • 39 citations

Substrate specificity of collagen proline hydroxylase: Hydroxylation of a specific proline residue in bradykinin

1969 • 38 citations

Isolation of hydroxylysyl-sRNA and hydroxyprolyl-sRNA in a chick embryo system

1963 • 38 citations

Chromatographic separation of the enzymes required for hydroxylation of lysine and proline residues of protocollagen

1971 • 38 citations

The polyribosomal synthesis of collagen

1967 • 36 citations

Hydroxylation of proline after the release of proline-rich polypeptides from ribosomal complexes during uninhibited collagen biosynthesis

1967 • 36 citations

Further Evidence for the Accumulation of a Hydroxyproline-deficient, Collagenase-degradable Protein during Collagen Biosynthesis in Vitro

1966 • 36 citations

Denatured Collagen from the Cuticle of Ascaris lumbricoides as a Substrate for Protocollagen Proline Hydroxylase

1968 • 35 citations

Hydralazine-induced disturbances in collagen biosynthesis

1972 • 35 citations

Association of prolyl hydroxylase activity with membranes

1973 • 34 citations

7-Hydroxylation of thymine in a neurospora strain coupled to oxidative decarboxylation of 2-ketoglutarate

1970 • 34 citations

Effect of cycloheximide on collagen biosynthesis as evidence for a post-ribosomal site for the hydroxylation of proline

1967 • 34 citations

The cell-free conversion of a deoxyribonucleoside to a ribonucleoside without detachment of the deoxyribose

1968 • 34 citations

α‐Ketoglutarate in biological hydroxylations

1968 • 33 citations

Collagen synthesis by isolated bone cells

1967 • 33 citations

Sequential steps in the synthesis of hydroxylysine and the glycosylation of hydroxylysine during the biosynthesis of collagen

1969 • 32 citations

The formation of 5-formyluracil by cell-free preparations from Neurospora crassa

1968 • 32 citations

Occurrence of Glycylhydroxypyrolyl Sequences in Earthworm Cuticle Collagen

1968 • 32 citations

The enzymic hydroxylation of protocollagen models

1969 • 31 citations

Increased lysine hydroxylation in rat bone and tendon collagen and localization of the additional residues

1973 • 31 citations

Soluble collagen proline hydroxylase and its substrates in several animal tissues.

1966 • 31 citations

Structure of Protocollagen Proline Hydroxylase from Chick Embryos

1973 • 30 citations

Partial purification and properties of collagen lysine hydroxylase from chick embryos

1972 • 30 citations

Increased protocollagen proline hydroxylase activity in synovial tissue in rheumatoid arthritis

1970 • 30 citations

Carbohydrate content of insoluble elastins prepared from adult bovine and calf ligamentum nuchae and tropoelastin isolated from copper-deficient procine aorta

1971 • 29 citations

Elevation of proline hydroxylase activity in diseased rabbit aorta

1969 • 27 citations

Studies pertaining to the purification and properties of thymine 7-hydroxylase

1971 • 27 citations

The Formation of Collagen α Chains in the Absence of Proline Hydroxylation

1970 • 26 citations

The biosynthesis of cis-4-hydroxy-L-proline in sandal (Santalum album L.)

1970 • 26 citations

Hydroxyproline Biosynthesis : Loss of Hydrogen during the Hydroxylation of Proline

1964 • 26 citations

Specificities of protocollagen hydroxylases from different sources

1969 • 26 citations

Labeled antibodies to protocollagen proline hydroxylase from chick embryos for intracellular localization of the enzyme

1972 • 25 citations

In Vitro Enzymatic Hydroxylation of Prolyl Residues in the α1-CB2 Fragment of Rat Collagen

1971 • 25 citations

Inhibition of collagen hydroxylysine formation by chelating agents

1967 • 24 citations

Aortic Connective Tissue Changes in Miniature Pigs Fed a Lipid-Rich Diet

1972 • 23 citations

Cell-free collagen biosynthesis and the hydroxylation of sRNA-proline

1965 • 23 citations

Independence of collagen hydroxylation on the conformational state of the precursor substrate

1968 • 23 citations

Studies on the stability of protocollagen hydroxylase

1968 • 21 citations

A general method for the rapid measurement of enzyme precursors by the displacement of enzyme from antibody: The measurement of collagen proline hydroxylase precursor in cultured fibroblasts

1972 • 20 citations

Conversion of C14-and cis-or trans-4-H3-L-proline to 4-hydroxy-L-proline and 4-oxo-L-proline by Streptomyces antibioticus.

1965 • 20 citations

Accumulation of collagen in the uterus of the immature rat administered estradiol-17β

1973 • 20 citations

The Incorporation of¹⁴C-Proline into the Proteins of Growing Cells: II. A NOTE ON EVIDENCE FROM CULTURED CARROT EXPLANTS BY ACRYLAMIDE GEL ELECTROPHORESIS

1963 • 20 citations

The absence of iron and certain coenzymes in highly purified protocollagen proline hydroxylase

1971 • 19 citations

Transfer of puromycin-containing polypeptides through the plasma membrane of cartilage cells synthesizing collagen.

1967 • 19 citations

Electron Microscopy of Protocollagen Proline Hydroxylase from Chick Embryos

1970 • 18 citations

Antibody to Prolyl Hydroxylase from Rat Skin and Its Cross-Reactivity with Enzyme from Other Species

1973 • 17 citations

Asymmetry in the hydroxylation of (Pro-Pro-Gly)5 by protocollagen proline hydroxylase

1971 • 17 citations

Protocollagen proline hydroxylase of the mouse uterus during pregnancy and post-partum involution

1970 • 17 citations

Studies on collagen metabolism with 18O as a tracer

1963 • 17 citations

Synthesis and Secretion of Hypoxyproline-containing Macromolecules in Carrots

1970 • 17 citations

Hydroxyprolyl-soluble ribonucleic acid and the biosynthesis of collagen

1966 • 15 citations

Connective tissue

1968 • 15 citations

Oxygen-18 and fluoroproline studies on the synthesis of hydroxyproline and oxoproline in actinomycin

1969 • 14 citations

Collagen proline hydroxylase activity in the uterus of the rat during rapid collagen synthesis in vivo

1973 • 13 citations

Inhibition of prolyl hydroxylase activity by bradykinin analogs containing a prolyl-like residue

1973 • 13 citations

Two forms of the effect of α,α′-dipyridyl and 1,10-phenanthroline on synthesized hydroxyproline-deficient collagen

1966 • 13 citations

Studies on bound trans-4-hydroxy-<scp>l</scp>-proline in sandal (Santalum album L.)

1970 • 12 citations

Aortic proline hydroxylase in hypoxia induced arteriosclerosis in rabbits

1972 • 12 citations

Hydroxylation of poly(l‐prolyl‐l‐prolylglycyl) of defined molecular weights by protocollagen proline hydroxylase

1969 • 11 citations

Distribution and seasonal variation of allohydroxy-<scp>l</scp>-proline in Santalum album L

1961 • 11 citations

Evidence for a low-molecular-weight collagen precursor.

1967 • 11 citations

A Peroxide Fragmentation Reaction

1967 • 8 citations

Syntheses and enzymic hydroxylation of protocollagen model peptides containing glutamyl or leucyl residue

1972 • 8 citations