Covalent Fluorescent Probes

LOCALIZATION OF ANTIGEN IN TISSUE CELLS

1950 • 1,908 citations

Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer

1978 • 1,308 citations

Fluorimetry study of N-(1-pyrenyl)iodoacetamide-labelled F-actin. Local structural change of actin protomer both on polymerization and on binding of heavy meromyosin.

1981 • 811 citations

Conjugation of antibodies with fluorochromes: Modifications to the standard methods

1976 • 757 citations

O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

1975 • 738 citations

Membrane proteins related to anion permeability of human red blood cells

1974 • 732 citations

The anion transport system of the red blood cell The role of membrane protein evaluated by the use of ‘probes’

1978 • 722 citations

Fluorimetry Study of N‐(1‐Pyrenyl)iodoacetamide‐Labelled F‐Actin

1981 • 694 citations

[12] Dansyl chloride procedure

1967 • 649 citations

The Reaction of Phenylglyoxal with Arginine Residues in Proteins

1968 • 636 citations

Conformation of Biopolymers

1967 • 463 citations

Rotational and lateral diffusion of membrane proteins

1979 • 458 citations

Surface density determination in membranes by fluorescence energy transfer

1978 • 422 citations

[56] Carbodiimide modification of proteins

1972 • 375 citations

Use of the Dansyl Reaction in Biochemical Analysis

1970 • 373 citations

7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and other amines

1968 • 352 citations

A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochrome-labelled RNA

1980 • 325 citations

Chemical Modification of Membranes

1971 • 304 citations

Studies on the reaction of fluorescamine with primary amines

1974 • 286 citations

Bimane fluorescent labels: labeling of normal human red cells under physiological conditions.

1979 • 253 citations

Segmental flexibility of the S-1 moiety of myosin

1973 • 233 citations

Practical fluorescence; theory, methods, and techniques,

1973 • 228 citations

A fluorescent label for the outer components of the plasma membrane

1964 • 216 citations

[7] The use of carbodiimides in the preparation of immunizing conjugates

1980 • 197 citations

Fluorescently labelled molecules as probes of the structure and function of living cells

1980 • 189 citations

Proximity Relationships in Rhodopsin

1972 • 188 citations

Fluorescence energy transfer between Ca²⁺ transport ATPase molecules in artificial membranes

1977 • 185 citations

A fluorescence stopped flow analysis of Ca2+ exchange with troponin C.

1979 • 185 citations

The reactivity of SH groups with a fluorogenic reagent

1970 • 177 citations

Fluorescence properties of o-phthaldialdehyde derivatives of amino acids

1979 • 173 citations

Reaction of Fluorescein-Isothiocyanate with Proteins and Amino Acids: I. Covalent and Non-Covalent Binding of Fluorescein-Isothiocyanate and Fluorescein to Proteins

1969 • 164 citations

N-(1-Pyrene)maleimide: a fluorescent crosslinking reagent

1976 • 156 citations

A fluorescent probe study of Ca2+ binding to the Ca2+-specific sites of cardiac troponin and troponin C.

1980 • 153 citations

N-(3-Pyrene)maleimide: a Long Lifetime Fluorescent Sulfhydryl Reagent

1973 • 153 citations

4-Bromomethyl-7-methoxycoumarin as a new fluorescence label for fatty acids

1977 • 152 citations

Molecular cytochemistry: incorporation of fluorescently labeled actin into living cells.

1978 • 149 citations

Mobility, clustering, and transport of nerve growth factor in embryonal sensory cells and in a sympathetic neuronal cell line.

1980 • 140 citations

Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF) I. Preparation and fractionation of labelled IgG

1976 • 137 citations

9-Diazomethylanthracene as a new fluorescence and ultraviolet label for the spectrometric detection of picomole quantities of fatty acids by high-pressure liquid chromatography

1980 • 137 citations

Fluorescence energy transfer between subfragment-1 and actin points in the rigor complex of actosubfragment-1

1979 • 133 citations

Anchorage of a band 3 population at the erythrocyte cytoplasmic membrane surface: protein rotational diffusion measurements.

1980 • 131 citations

Effects of ATP and protons on the Na : K selectivity of the (Na+ + K+)-ATPase studied by ligand effects on intrinsic and extrinsic fluorescence

1980 • 130 citations

An assay method for disulfide groups by fluorescence quenching

1964 • 129 citations

Dansylaziridine-labeled troponin C. A fluorescent probe of Ca2+ binding to the Ca2+-specific regulatory sites.

1978 • 123 citations

Fluorometric assay of secondary amino acids

1973 • 121 citations

Distribution of fluorescently labeled actin in living sea urchin eggs during early development.

1979 • 121 citations

Location of the stilbenedisulfonate binding site of the human erythrocyte anion-exchange system by resonance energy transfer

1979 • 120 citations

Mechanism of quenching of fluorescein by anti-fluorescein IgG antibodies

1977 • 116 citations

Rotational diffusion of cell surface components by time-resolved phosphorescence anisotropy.

1979 • 105 citations

Serine esterase inhibitors block stimulus-induced mobilization of arachidonic acid and phosphatidylinositide-specific phospholipase C activity in platelets.

1980 • 105 citations

The excimer fluorescence of pyrene-labeled tropomyosin. A probe of conformational dynamics.

1980 • 103 citations

Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas.

1980 • 97 citations

New fluorochromes for thiols: maleimide and iodoacetamide derivatives of a 3-phenylcoumarin fluorophore.

1981 • 94 citations

A spectroscopic technique for measuring slow rotational diffusion of macromolecules. 1: Preparation and properties of a triplet probe

1976 • 94 citations

Fluorescent protein-dye conjugates

1969 • 92 citations

Fluorescence energy transfer in myosin subfragment-1

1980 • 88 citations

PARTIAL PURIFICATION OF SOLUBLE RNA

1960 • 87 citations

A new technique for the fluorescent labelling of proteins

1960 • 87 citations

Fluorescence and structures of proteins as measured by incorporation of fluorophore

1970 • 86 citations

Distribution of fluorescently labeled actin and tropomyosin after microinjection in living tissue culture cells as observed with TV image intensification

1980 • 84 citations

Fluorescence energy transfer on acetylcholinesterase: spatial relationship between peripheral site and active center

1980 • 83 citations

N-dansylaziridine: A new fluorescent modification for cysteine thiols

1974 • 80 citations

Distances between 3' ends of ribosomal ribonucleic acids reassembled into Escherichia coli ribosomes

1980 • 80 citations

Localization of Membrane-Associated Proteins in Vesicular Stomatitis Virus by Use of Hydrophobic Membrane Probes and Cross-Linking Reagents

1980 • 79 citations

Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin.

1980 • 78 citations

Fluorescent thin-layer peptide mapping for protein identification and comparison in the subnanomole range

1978 • 75 citations

Defining the “fast-reacting” thiols of myosin by reaction with 1,5 IAEDANS

1976 • 72 citations

Fluorescent thiol reagents. VI. N-(1-Anilinonaphthyl-4)maleimide; a fluorescent hydrophobic probe directed to thiol groups in protein.

1973 • 72 citations

Fluorescence Polarization of Pyrenebutyric-Bovine Serum Albumin and Pyrenebutyric-Human Macroglobulin Conjugates

1969 • 69 citations

Dimethyl(2-hydroxy-5-nitrobenzyl)sulfonium Salts

1970 • 69 citations

The use of NBD chloride (7 chloro-4-nitrobenzo-2-oxa-1,3-diazole) in detecting amino acids and as an N-terminal reagent

1973 • 69 citations

Aryl azide photolabels in biochemistry

1980 • 69 citations

The formation of 1-dimethylaminonaphthalene-5-sulphonamide during the preparation of 1-dimethylaminonaphthalene-5-sulphonylamino acids

1965 • 66 citations

Reaction of chicken egg white lysozyme with 7-chloro-4-nitrobenz-2-oxa-1,3-diazole

1973 • 63 citations

Reactions of cysteine, its derivatives, glutathione, coenzyme A, and dihydrolipoic acid with isothiocyanates

1979 • 63 citations

Crosslinking and labeling of membrane proteins by transglutaminase-catalyzed reactions.

1975 • 62 citations

Studies of Transfer RNA Tertiary Structure by Singlet-Singlet Energy Transfer

1974 • 62 citations

Selective photoaffinity labeling of acetylcholine receptor using a cholinergic analog

1977 • 61 citations

Fluorescent thiol reagents. VI

1973 • 61 citations

Synthesis of a fluorescent boronic acid which reversibly binds to cell walls and a diboronic acid which agglutinates erythrocytes

1980 • 60 citations

The reaction of 1-dimethylaminonaphthalene-5-sulfonyl chloride (DANSCl) with erythrocyte membranes A new look at “vectorial” membrane probes

1973 • 59 citations

Chemical modification and fluorescence studies of chloroplast coupling factor

1977 • 58 citations

Dansylcadaverine specific staining for transamidating enzymes

1979 • 55 citations

Synthesis, separation and characterization of the mono- and diazide analogs of ethidium bromide

1977 • 54 citations

Spectroscopic properties of ethidium monoazide: a fluorescent photoaffinity label for nucleic acids

1978 • 52 citations

Labeling of human erythrocyte membranes with eosin probes used for protein diffusion measurements. Inhibition of anion transport and photo-oxidative inactivation of acetylcholinesterase

1979 • 52 citations

Anisotropy Decay of Labelled Actin

1979 • 52 citations

Reaction of purified (Na,K)-ATPase with the fluorescent sulfhydryl probe 2-(4'-maleimidylanilino)naphthalene 6-sulfonic acid. Characterization and the effects of ligands.

1979 • 51 citations

The G-actin → F-actin transformation as studied by the fluorescence of bound dansyl cystine

1971 • 50 citations

Fluorescent reagents for the labeling of glycoconjugates in solution and on cell surfaces

1980 • 49 citations

1-Dimethylaminonaphthalene-5-sulfonyl hydrazine (dansyl hydrazine): A fluorometric reagent for carbonyl compounds

1971 • 49 citations

Transglutaminase-catalyzed insertion of a fluorescent probe into the protease-sensitive region of rhodopsin

1978 • 48 citations

A method for linking fluorescent labels to polynucleotides: application to studies of ribosome-ribonucleic acid interactions

1980 • 47 citations

Use of substrates with fluorescent donor and acceptor chromophores for the kinetic assay of hydrolases

1973 • 47 citations

Synthesis and fluorescent properties of some N-methyl-2-anilino-6-naphthalenesulfonyl derivatives

1968 • 47 citations

A new RNA-RNA crosslinking reagent and its application to ribosomal 5S RNA

1978 • 46 citations

Fluorescent labeling of proteins and a plasma membrane using cycloheptaamylose-dansyl chloride complex

1974 • 46 citations

Investigation of the active site of papain with fluorescent probes

1973 • 45 citations

Crosslinkage and visualization of acetylcholine receptors on myotubes with biotinylated alpha-bungarotoxin and fluorescent avidin.

1980 • 45 citations

Singlet-singlet energy transfer studies of the internal organization of nucleosomes

1980 • 45 citations

Hydrolysis of N-substituted maleimides: Stability of fluorescence thiol reagents in aqueous media.

1977 • 45 citations

Fluorescent phosphonate label for serine hydrolases, pyrenebutyl methylphosphonofluoridate: reaction with acetylcholinesterase

1978 • 42 citations

The Reaction of Dimethyl Sulfoxide and 5-Dimethylaminonaphthalene-1-sulfonyl Chloride

1966 • 41 citations

Covalent attachment of fluorescent groups to transfer ribonucleic acid. Reactions with 4-bromomethyl-7-methoxy-2-oxo-2H-benzopyran

1974 • 41 citations

New fluorescent hydrazide reagents for the oxidized 3'-terminus of RNA

1974 • 41 citations

The reaction of rabbit muscle creatine kinase with some derivatives of iodoacetamide

1979 • 40 citations

The Rotational Diffusion of Thyroglobulin

1969 • 40 citations

Bestimmung von Thiolverbindungen durch selektive Fluorescenzderivatisierung und HPLC

1979 • 40 citations

Affinity of intact Escherichia coli for hydrophobic membrane probes is a function of the physiological state of the cells.

1977 • 39 citations

Fluorimetric studies of actin labeled with dansyl aziridine

1978 • 38 citations

Sequence-specific crosslinking agents for nucleic acids

1978 • 38 citations

Intracellular dye-marked enkephalin neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus.

1979 • 38 citations

New automated fluorometric peptide microassay for carnosine in mouse olfactory bulb

1978 • 36 citations

Sulfhydryl-specific fluorescent label, S-mercuric N-dansylcysteine. Titrations of glutathione and muscle proteins

1974 • 36 citations

Pulse‐Fluorometry Study on Actin and Heavy Meromyosin Using F‐Actin Labelled with N‐(1‐Pyrene)maleimide

1980 • 35 citations

Specific fluorescent derivatives of macromolecules reaction of dansyl fluoride with serine proteinases

1976 • 34 citations

Energy transfer distance measurements in immunoglobulins

1973 • 33 citations

The histochemical application of dansylhydrazine as a fluorescent labeling reagent for sialic acid in cellular glycoconjugates

1975 • 33 citations

Introduction of a fluorescent label into the carbohydrate moiety of glycoconjugates

1975 • 32 citations

Motion of myosin fragments during actin‐activated ATPase: fluorescence correlation spectroscopy study

1979 • 32 citations

The Conjugation of Amino Acids with Isocyanates of the Anthracene and 1,2-Benzanthracene Series

1939 • 31 citations

Alkylation and Cleavage of Methionine Peptides

1962 • 30 citations

Chromatographically pure fluorescein and tetramethylrhodamine isothiocyanates

1961 • 29 citations

Resonance energy transfer between catalytic sites of bovine liver uridine diphosphoglucose dehydrogenase

1980 • 29 citations

Conformational changes of purified (Na+ + K+)-ATPase detected by a sulfhydryl fluorescence probe

1977 • 29 citations

Fluorescent photoaffinity labeling: Adenosine 3′,5′-cyclic monophosphate receptor sites

1978 • 29 citations

Fluorescent photochemical surface labeling of intact human erythrocytes

1979 • 28 citations

Fluorescent labeling of the surface proteins of erythrocyte membranes using cycloheptaamylose-fluorescamine complex

1975 • 28 citations

Myosin structure. Proximity measurements by fluorescence energy transfer

1975 • 27 citations

4-hydroxy-3-nitrophenylglyoxal. A chromophoric reagent for arginyl residues in proteins

1979 • 26 citations

Ribosome binding by tRNAs with fluorescent labeled 3′ termini

1980 • 25 citations

The effect of modifying lysine-126 on the physical, catalytic and regulatory properties of bovine liver glutamate dehydrogenase

1973 • 25 citations

The Photo-decomposition of 1-Azidopyrene in the Benzene Solution

1972 • 25 citations

Active site flourescent labeled dansyl and anthraniloyl human thrombins

1978 • 25 citations

The site at which 4-iodoacetamidosalicylate reacts with glutamate dehydrogenases

1973 • 25 citations

New Fluorescence Edman Reagent for Microanalysis of Peptides

1970 • 24 citations

A membrane-bound fluorescent probe to detect phospholipid vesicle-cell fusion

1980 • 24 citations

Fluorescence properties and reactions of a novel fluorescence thiol reagent, N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide(DACM).

1977 • 24 citations

Specific fluorescent labeling of chicken myofibril Z-line proteins catalyzed by guinea pig liver transglutaminase.

1979 • 24 citations

Sulfhydryl Groups on the Spermatozoa Membrane. A Study with a New Fluorescent Probe for SH Group1

1976 • 23 citations

Oligosaccharide motion in erythrocyte membranes investigated by picosecond fluorescence polarization and microsecond dichroism of an optical probe.

1980 • 23 citations

Evidence that binding to the S2-subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25–histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a hydrophobic side chain

1979 • 22 citations

Covalent labelling of specific membrane carbohydrate residues with fluorescent probes

1980 • 22 citations

On the covalent binding of mRNA models to the part of the 16 S RNA which is located in the mrna binding site of the 30 S ribosome

1975 • 22 citations

Fluorescent analogs of insecticides and synergists. Synthesis and reactions of active-site-directed fluorescent probes

1971 • 22 citations

Demonstration of sulfhydryl and disulfide groups by a fluorescent maleimide procedure

1980 • 21 citations

Fluorescence studies of the location of proteins L7 and L12 on the Escherichia coli ribosome.

1981 • 19 citations

Labeling of the active site of glutamate dehydrogenase with a photogenerated species

1973 • 19 citations

Pyrene derivatives as fluorescent probes of conformation near the 3′ termini of polyribonucleotides

1977 • 19 citations

Covalent attachment of fluorescent probes to the X-base of Escherichia coli phenylalanine transfer ribonucleic acid

1977 • 18 citations

Studies on calcium ion-induced conformation changes in the actin-tropomyosin-troponin system by fluorimetry

1974 • 18 citations

Gel electrophoresis of fluorescent labeled cyanogen bromide cleavage products at the submicrogram level

1979 • 18 citations

Interaction of a new fluorescent reagent with sulfhydryl groups of the (Na++K+)-stimulated ATPase

1976 • 17 citations

Fluorescent photoaffinity labeling of F1 ATPase from Micrococcus luteus with 8-azido-1,N6-etheno-adenosine 5′-triphosphate

1980 • 17 citations

A novel fluorescent thiol reagent: Syntheses and electronic spectra of N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM-3) and the related compounds.

1977 • 17 citations

Anomalous reaction of 4-chloro-7-nitrobenzofurazan with thiol compounds

1979 • 16 citations

Conformational aspects of rhodopsin and retinal disc membranes

1973 • 16 citations

Dansylation of amines, phenolic and catecholic amines and amino acids in aprotic solvents

1978 • 16 citations

Synthesis and properties of 8-azido-1,N⁶-etheno adenosine triphosphate — a fluorescent and photosensitive ATP analog

1978 • 15 citations

Über ϵ-Markierung von Peptiden. Automatische Sequenzanalyse des Insulins

1973 • 15 citations

Efficiency of photolytic binding of ethidium monoazide to nucleic acids and synthetic polynucleotides.

1979 • 15 citations

Fluorescence labeling of the human erythrocyte anion transport system. Subunit structure studied with energy transfer

1979 • 14 citations

Fluorescent tracer method for protein SH groups. II

1978 • 14 citations

Studies of the ADP/ATP carrier of mitochondria with fluorescent ADP analogue formycin diphosphate

1979 • 14 citations

Electrophoretic Injection of a Fluorescent Dye into Giant Mitochondria and Mitoplasts

1980 • 13 citations

The preparation and characterization of some novel fluorescence labels derived from 7-substituted 2H-1-benzopyrane-2-ones

1979 • 13 citations

Absorption, Fluorescence, and Linear Dichroism Spectra of Fluorescein Mercuric Acetate (FMA) bound to F-actin

1979 • 12 citations

Neutral and cationic sulfonamido derivatives of the fluorescent probe 2-p-toluidinylnaphthalene-6-sulfonate. Properties and mechanistic implications

1975 • 12 citations

Conformational flexibility and structure of creatine kinase

1975 • 12 citations

Spin-labeled polyribonucleotides

1980 • 11 citations

Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes

1979 • 10 citations

Fluorescence thiol reagents: Syntheses and properties of N-acridinylmaleimides and their derivatives.

1978 • 10 citations

Alkaline structural transition of 4-nitrobenz-2-oxa-1,3-diazolyl-Lysozyme. Kinetic and spectroscopic investigations.

1977 • 10 citations

A new fluorescent label for antibody proteins

1960 • 10 citations

Preparation and characterization of 3-azido-2,7-naphthalene disulfonate: A photolabile fluorescent precursor useful as a hydrophilic surface probe

1980 • 9 citations

Interaction of dansylated peptidyl chloromethanes with trypsin, chymotrypsin, elastase, and thrombin

1980 • 9 citations

Energy transfer distance measurements in immunoglobulins

1974 • 8 citations

Fluorescent Probes for Antibody Active Sites: I. Production of Antibodies Specific to the N-Methyl-2-anilinonaphthalene-6-sulfonyl Group in Rabbits and Some Fluorescent Properties of the Hapten Bound to the Antibodies1

1976 • 8 citations

Screening search for fluorescent thiol reagents: Syntheses of N-naphthylmaleimide derivatives with a dimethylamino or a methoxy group as an auxochrome and their electronic spectra.

1976 • 8 citations

An extended series of fluorescent sulfhydryl reagents useful in energy transfer measurements

1978 • 8 citations

Pyrenesulfonyl azide: A covalent probe permitting invitro desensitization of labeled acetylcholine-rich membrane fragments from Torpedocalifornica

1979 • 7 citations

Fluorescent reagent for the periodic acid-Schiff and Feulgen reaction for cytochemical studies.

1976 • 7 citations

The Synthesis of Mono- and Bifunctional Nitriles and Imidoesters Carrying a Fluorescent Group

1975 • 7 citations

The Structures of Tetramethylrhodamine Isothiocyanate Isomers: An N. M. R. Study

1968 • 5 citations

A Fluorescence Stopped-Flow Study on Troponin Labeled with N-Ethyl Maleimide and N p-2(-Benzimidazolyl)phenyl) Maleimide 1

1979 • 5 citations

Staining of Blood Cells with Periodic Acid/Salicyloyl Hydrazide (PA-SH)

1966 • 5 citations

Theory, Methods and Techniques

1991 • 4 citations

Chemistry of the phenanthrenequinone (PEQ) test for monosubstituted guanidines

1976 • 3 citations

Reconstitution and electron spin resonance spin labeling studies of nucleosomes

1980 • 2 citations

Aminoacyl derivatives of nucleosides, nucleotides, and polynucleotides

1977 • 2 citations

Photoimmobilization of acetylcholinesterase on 5-azido-1-naphthalenesulfonyl substituted glass beads and kinetic studies on the immobilized enzyme

1980 • 1 citations