Biophysical Studies of Calmodulin

Calmodulin Plays a Pivotal Role in Cellular Regulation

1980 • 2,441 citations

Kinetics and Mechanism

1961 • 2,376 citations

Why genes in pieces?

1978 • 2,256 citations

Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.

1982 • 2,255 citations

Physical chemistry of macromolecules

1962 • 2,105 citations

Biophysical chemistry

1979 • 1,709 citations

Carp Muscle Calcium-binding Protein

1973 • 1,359 citations

Three-dimensional structure of calmodulin

1985 • 1,035 citations

Ca2+-induced hydrophobic site on calmodulin: Application for purification of calmodulin by phenyl-Sepharose affinity chromatography

1982 • 877 citations

THE HEMOGLOBIN SYSTEM

1925 • 813 citations

Calmodulin

1982 • 771 citations

The complete amino acid sequence of the Ca2+-dependent modulator protein (calmodulin) of bovine brain.

1980 • 588 citations

Binding of trifluoperazine to the calcium-dependent activator of cyclic nucleotide phosphodiesterase.

1977 • 544 citations

Calcium-induced exposure of a hydrophobic surface on calmodulin

1980 • 520 citations

Energetics of Ligand Binding to Proteins

1975 • 519 citations

Physiological implications of the presence, distribution, and regulation of calmodulin in eukaryotic cells.

1982 • 516 citations

Physicochemical properties of rat testis Ca2+-dependent regulator protein of cyclic nucleotide phosphodiesterase. Relationship of Ca2+-binding, conformational changes, and phosphodiesterase activity

1977 • 459 citations

Hydrophobic regions function in calmodulin-enzyme(s) interactions.

1980 • 363 citations

Positive cooperative binding of calcium to bovine brain calmodulin

1980 • 334 citations

Effects of cations on affinity of calmodulin for calcium: ordered binding of calcium ions allows the specific activation of calmodulin-stimulated enzymes. Theoretical approach to study of multiple ligand binding to a macromolecule

1981 • 321 citations

Structure of vitamin D-dependent calcium-binding protein from bovine intestine

1981 • 309 citations

R 24571: A new powerful inhibitor of red blood cell Ca++-transport ATPase and of calmodulin-regulated functions

1981 • 260 citations

Purification of plant calmodulin by fluphenazine-Sepharose affinity chromatography

1979 • 252 citations

Conversion of 3-nitrotyrosine to 3-aminotyrosine in peptides and proteins

1967 • 252 citations

Interaction of the antihypertensive drug felodipine with calmodulin

1981 • 251 citations

Aluminum interaction with calmodulin

1983 • 216 citations

Calcium-dependent affinity chromatography of calmodulin on an immobilized phenothiazine

1979 • 216 citations

Calcium- and magnesium-dependent conformational states of calmodulin as determined by nuclear magnetic resonance

1980 • 204 citations

Ca2+-dependent hydrophobic-interaction chromatography. Isolation of a novel Ca2+-binding protein and protein kinase C from bovine brain

1984 • 202 citations

Agonist and antagonist properties of calmodulin fragments.

1984 • 198 citations

Chicken calmodulin genes. A species comparison of cDNA sequences and isolation of a genomic clone.

1983 • 187 citations

Sequence homology of the Ca2+-dependent regulator of cyclic nucleotide phosphodiesterase from rat testis with other Ca2+-binding proteins.

1978 • 179 citations

Structural changes in melittin and calmodulin upon complex formation and their modulation by calcium

1983 • 176 citations

Activity-structure relationship of calmodulin antagonists, Naphthalenesulfonamide derivatives.

1981 • 163 citations

Stimulation of the purified erythrocyte Ca2+-ATPase by tryptic fragments of calmodulin.

1984 • 151 citations

Thermodynamic study of domain organization in troponin C and calmodulin

1985 • 148 citations

Characterization of tryptic fragments obtained from bovine brain protein modulator of cyclic nucleotide phosphodiesterase.

1977 • 142 citations

Phenothiazine antagonism of calmodulin: A structurally-nonspecific interaction

1981 • 141 citations

Structural information from NMR secondary chemical shifts of peptide α C-H protons in proteins

1983 • 139 citations

Protein interactions with small molecules

1974 • 139 citations

Calcium-dependent affinity chromatography of S-100 and calmodulin on calmodulin antagonist-coupled Sepharose.

1981 • 131 citations

Identification of multiple species of calmodulin messenger RNA using a full length complementary DNA.

1983 • 131 citations

A fluorescent calmodulin that reports the binding of hydrophobic inhibitory ligands

1983 • 129 citations

Structural and functional properties of calmodulin from the eukaryotic microorganism Dictyostelium discoideum

1984 • 129 citations

The Amino Acid Sequence of the δ Subunit (Calmodulin) of Rabbit Skeletal Muscle Phosphorylase Kinase

1981 • 128 citations

A ¹¹³Cd NMR study of calmodulin and its interaction with calcium, magnesium and trifluoperazine

1980 • 127 citations

Metal ion and drug binding to proteolytic fragments of calmodulin: proteolytic cadmium-113 and proton nuclear magnetic resonance studies

1984 • 125 citations

Calcium-dependent interaction of S100b, troponin C, and calmodulin with an immobilized phenothiazine.

1981 • 123 citations

Quantitation of energy coupling between Ca2+, calmodulin, skeletal muscle myosin light chain kinase, and kinase substrates.

1984 • 120 citations

Cadmium-113 nuclear magnetic resonance studies of proteolytic fragments of calmodulin: assignment of strong and weak cation binding sites

1983 • 119 citations

The amino acid sequence of the Tetrahymena calmodulin which specifically interacts with guanylate cyclase

1981 • 116 citations

Calcium binding domains of calmodulin. Sequence of fill as determined with terbium luminescence.

1982 • 113 citations

Metal-ion-dependent hydrophobic-interaction chromatography of α-lactalbumins

1984 • 112 citations

Determination of the free-energy coupling for binding of calcium ion and troponin I to calmodulin

1982 • 110 citations

The kinetics of calcium binding to calmodulin: Quin 2 and ANS stopped-flow fluorescence studies

1984 • 109 citations

Protein affinities for small molecules: Conceptions and misconceptions

1979 • 108 citations

Peptide binding by calmodulin and its proteolytic fragments and by troponin C

1984 • 106 citations

Human erythrocyte calmodulin. Further chemical characterization and the site of its interaction with the membrane.

1979 • 105 citations

¹H‐NMR studies of calmodulin

1984 • 103 citations

Techniques in calcium research

1983 • 99 citations

Tyrosine fluorescence of ram testis and octopus calmodulins. Effects of calcium, magnesium, and ionic strength

1981 • 97 citations

Similarity in Ca2+-induced changes between troponin-C and protein activator of 3′:5′-cyclic nucleotide phosphodiesterase and their tryptic fragments

1977 • 96 citations

Chemical modification studies on the Ca²⁺ ion-dependent protein modulator of cyclic nucleotide phosphodiesterase

1977 • 95 citations

A study of calmodulin and its interaction with trifluoperazine by high resolution ¹H NMR spectroscopy

1981 • 94 citations

Metal‐Binding Properties of Calmodulin

1982 • 94 citations

N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a calmodulin antagonist, also inhibits phospholipid sensitive calcium-dependent protein kinase

1983 • 93 citations

¹H NMR studies of calmodulin

1984 • 93 citations

Tryptic fragments of calmodulin. Ca2+- and Mg2+-induced conformational changes.

1982 • 92 citations

Regulation of brain cyclic nucleotide phosphodiesterase by calmodulin. A quantitative analysis.

1981 • 92 citations

Calcium‐dependent hydrophobic interaction chromatography of calmodulin, troponin C and their proteolytic fragments

1983 • 92 citations

Binding of hormones and neuropeptides by calmodulin

1983 • 92 citations

Circular dichroism studies on Ca2+-dependent protein modulator oxidized with N-chlorosuccinimide.

1978 • 91 citations

Tissue-specific expression of a chicken calmodulin pseudogene lacking intervening sequences.

1983 • 91 citations

Chemical modification studies on the Ca2+-dependent protein modulator: the role of methionine residues in the activation of cyclic nucleotide phosphodiesterase.

1978 • 90 citations

Complete amino acid sequence of human brain calmodulin

1982 • 89 citations

Influence of Ca²⁺ and Trifluoperazine on the Structure of Calmodulin

1982 • 86 citations

Sequential conformational changes in calmodulin upon binding of calcium

1984 • 85 citations

Terbium as luminescent probe of calmodulin calcium‐binding sites

1980 • 85 citations

Characterization of the Ca²⁺ Binding Sites of Calmodulin from Bovine Testis Using ⁴³Ca and ¹¹³Cd NMR

1982 • 84 citations

Nuclear magnetic resonance studies on calmodulin: calcium-induced conformational change

1983 • 82 citations

Preparation of azidocalmodulin: a photoaffinity label for calmodulin-binding proteins.

1981 • 82 citations

3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine, a hydrophobic, photoreactive probe, labels calmodulin and calmodulin fragments in a calcium(2+)-dependent way

1984 • 80 citations

Thermodynamics of Ca2+ binding to troponin-C.

1977 • 80 citations

A Thermodynamic Analysis of the Binding of Calcium, and Magnesium Ions to Parvalbumin

1980 • 78 citations

CRYSTALLOGRAPHIC STUDIES OF CALMODULIN AND HOMOLOGS

1980 • 77 citations

Chemical modification studies on the Ca²⁺ ion-dependent protein modulator: the role of methionine residues in the activation of cyclic nucleotide phosphodiesterase. Appendix: Circular dichroism studies on Ca²⁺ ion-dependent protein modulator oxidized with N-chlorosuccinimide

1978 • 76 citations

Stimulation of enzyme activities by fragments of calmodulin

1981 • 75 citations

Conformation-dependent nitration of the protein activator of cyclic adenosine 3',5'-monophosphate phosphodiesterase

1978 • 75 citations

The amino acid sequence of the calmodulin obtained from sea anemone (Metridium senile) muscle

1980 • 74 citations

Terbium binding to octopus calmodulin provides the complete sequence of ion binding

1980 • 73 citations

Calmidazolium and compound 48/80 inhibit calmodulin-dependent protein phosphorylation and ATP-dependent Ca2+ uptake but not Ca2+-ATPase activity in skeletal muscle sarcoplasmic reticulum.

1984 • 71 citations

¹¹³Cd NMR in the study of calcium binding proteins: Troponin C

1979 • 68 citations

Amino Acid Sequence of Calmodulin from Scallop (Patinopecten) Adductor Muscle1

1981 • 67 citations

Nuclear magnetic resonance studies on calmodulin: calcium-dependent spectral change of proteolytic fragments

1984 • 66 citations

The binding of calmodulin to myelin basic protein and histone H2B

1980 • 66 citations

STRUCTURE AND FUNCTION RELATIONSHIPS AMONG CALMODULINS AND TROPONIN C‐LIKE PROTEINS FROM DIVERGENT EUKARYOTIC ORGANISMS*

1980 • 65 citations

Thermodynamic investigations of proteins

1978 • 62 citations

Handbook of Metal Ligand Heats and Related Thermodynamic Quantities

1970 • 61 citations

Tyrosine and tyrosinate fluorescence of bovine testes calmodulin: calcium and pH dependence

1984 • 59 citations

Zinc‐dependent affinity chromatography of the S100b protein on phenyl—Sepharose

1982 • 58 citations

Calmodulin and intracellular Ca2+ receptors

1983 • 58 citations

Calcium ion-dependent covalent modification of calmodulin with norchlorpromazine isothiocyanate

1983 • 58 citations

Specific perturbation by Ca2+ of tyrosyl residue 138 of calmodulin.

1979 • 58 citations

Demonstration by covalent cross-linking of a specific interaction between beta-endorphin and calmodulin.

1983 • 57 citations

Ion binding to calmodulin

1983 • 55 citations

Biological activities of the peptides obtained by digestion of troponin C and calmodulin with thrombin

1981 • 53 citations

A thermodynamic and electron paramagnetic resonance study of structural changes in calmodulin induced by aluminum binding

1983 • 51 citations

Simultaneous determination of ΔG, ΔH and ΔS by an automatic microcalorimetric titration technique. Application to protein ligand binding

1982 • 48 citations

Localization of hydrophobic sites in calmodulin and skeletal muscle troponin C studied using tryptic fragments a simple method of their preparation

1983 • 47 citations

A¹¹³Cd and ¹H NMR Study of the Interaction of Calmodulin with D600, Trifluoperazine and Some Other Hydrophobic Drugs

1983 • 47 citations

Heat Capacity and Entropy Changes of Calmodulin Induced by Calcium Binding1

1984 • 46 citations

Conformation-dependent acetylation and nitration of the protein activator of cyclic adenosine 3',5'-monophosphate phosphodiesterase. Selective nitration of tyrosine residue 138

1978 • 46 citations

Fluorescence investigations of calmodulin hydrophobic sites

1984 • 45 citations

43Ca NMR studies of calcium binding to proteins: Interpretation of experimental data by bandshape analysis

1983 • 45 citations

Allosteric interactions among drug binding sites on calmodulin

1983 • 44 citations

Identification and purification of a phenothiazine binding fragment from bovine brain calmodulin

1982 • 42 citations

High field proton NMR studies of tryptic fragments of calmodulin: A comparison with the native protein

1984 • 41 citations

Calcium and Cadmium Binding to Troponin C

1983 • 39 citations

Calmodulin antagonists inhibit activity of myosin light‐chain kinase independent of calmodulin

1984 • 36 citations

Affinity chromatography of brain cyclic nucleotide phosphodiesterase using 3-(2-pyridyldithio)propionyl-substituted calmodulin linked to thiol-Sepharose

1983 • 35 citations

Molecular dynamics of calmodulin as monitored by fluorescence anisotropy

1982 • 34 citations

43Ca NMR studies of Ca2+-Tetrahymena calmodulin complexes

1982 • 34 citations

The Changes in Heat Capacity and Entropy of Troponin C Induced by Calcium Binding1

1982 • 31 citations

Carboxylmethylation of phosphodiesterase attenuates its activation by ca2+-calmodulin.

1984 • 30 citations

A survey of structural studies on calmodulin

1981 • 29 citations

Nuclear magnetic resonance studies on calmodulin: spectral assignments in the calcium-free state

1983 • 29 citations

A Calorimetric Study of Ca2+- and Mg2+-Binding by Calmodulin1

1983 • 28 citations

Calcium ion binding to pancreatic phospholipase A2 and its zymogen: a calcium-43 NMR study

1984 • 28 citations

Calmodulin interacts with cyclic nucleotide phosphodiesterase and calcineurin by binding to a metal ion-independent hydrophobic region on these proteins.

1983 • 27 citations

Rapid kinetic studies on calcium interactions with native and fluorescently labeled calmodulin

1981 • 25 citations

Calcium-dependent hydrophobic chromatography of calmodulin, S-100 protein and troponin-C

1984 • 24 citations

Studies of calmodulin structure: laser Raman spectroscopy of biomolecules. XVII

1983 • 24 citations

Affinity labeling of calmodulin-binding components in canine cardiac sarcoplasmic reticulum.

1982 • 23 citations

A study of the interactions between residues in the C‐terminal half of calmodulin by one and two‐dimensional NMR methods and computer modelling

1984 • 23 citations

Calmodulin binding to rat adipocyte plasma membrane: characterization and photoaffinity crosslinking of calmodulin to binding proteins

1982 • 22 citations

Influence of temperature and denaturing agents on the structural stability of calmodulin

1983 • 22 citations

Trifluoperazine binding to calmodulin: A shift reagent 43Ca NMR study

1984 • 21 citations

The utility of the nitrotyrosine chromophore as a spectroscopic probe in troponin C and modulator protein

1979 • 20 citations

SPIN LABELED CALMODULIN: A NEW PROBE FOR STUDYING Ca²⁺ AND MACROMOLECULAR INTERACTIONS*

1980 • 18 citations

Enthalpy, entropy and heat capacity changes induced by binding of calcium ions to cardiac troponin C

1983 • 17 citations

The dependence of the molecular dynamics of calmodulin upon pH and ionic strength

1983 • 17 citations

Thermodynamics of the binding of Ca2+ to porcine pancreatic phospholtase A2

1984 • 15 citations

Binding of a spin-labelled chlorpromazine analogue to calmodulin

1984 • 14 citations

Location of a binding site for 1-anilinonaphthalene-8-sulfonate on calmodulin

1984 • 13 citations

Preparation of an enzymatically active crosslinked complex between brain cyclic nucleotide phosphodiesterase and 3-(2-pyridyldithio)propionyl-substituted calmodulin

1984 • 10 citations

Preparation and properties of calcium-dependent resins with increased selectivity for calmodulin

1983 • 10 citations

Rapid kinetic studies of calmodulin interactions with calcium and troponin I as monitored by anthroylcholine fluorescence

1982 • 8 citations

Electron paramagnetic resonance study on calmodulin: conformational change and interaction with divalent cations

1983 • 6 citations

Temperature dependent conformational changes in calmodulin

1983 • 6 citations

Isolation, purification and cell-free synthesis of calmodulin from the pig anterior pituitary gland

1984 • 5 citations