A human beta-globin gene fused to the human beta-globin locus control region is expressed at high levels in erythroid cells of mice engrafted with retrovirus-transduced hematopoietic stem cells

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Genomic sequencing.

1984 • 8,343 citations

Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

1982 • 2,753 citations

Position-independent, high-level expression of the human β-globin gene in transgenic mice

1987 • 1,955 citations

Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus

1983 • 1,857 citations

Redesign of Retrovirus Packaging Cell Lines To Avoid Recombination Leading to Helper Virus Production

1986 • 1,276 citations

Nucleotide sequence of Moloney murine leukaemia virus

1981 • 1,079 citations

Identification of two distinct regulatory regions adjacent to the human β-interferon gene

1983 • 1,076 citations

Construction and applications of a highly transmissible murine retrovirus shuttle vector

1984 • 1,020 citations

Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges.

1988 • 908 citations

Electroporation for the efficient transfection of mammalian cells with DNA

1987 • 856 citations

Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

1985 • 818 citations

The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene

1980 • 556 citations

Progress Toward Human Gene Therapy

1989 • 484 citations

Globin gene regulation and switching: Circa 1990

1990 • 463 citations

Erythroid-specific expression of human beta-globin genes in transgenic mice.

1985 • 436 citations

SAMPLE PREPARATION FROM BLOOD, CELLS, AND OTHER FLUIDS

1990 • 427 citations

A dominant control region from the human β-globin locus conferring integration site-independent gene expression

1989 • 346 citations

Lineage-specific expression of a human β-globin gene in murine bone marrow transplant recipients reconstituted with retrovirus-transduced stem cells

1988 • 341 citations

Combination of interleukins 3 and 6 preserves stem cell function in culture and enhances retrovirus-mediated gene transfer into hematopoietic stem cells.

1989 • 316 citations

A single erythroid-specific DNase I super-hypersensitive site activates high levels of human beta-globin gene expression in transgenic mice.

1989 • 269 citations

Y-encoded, species-specific DNA in mice: Evidence that the Y chromosome exists in two polymorphic forms in inbred strains

1984 • 233 citations

Molecular analysis of the human beta-globin locus activation region.

1989 • 218 citations

DNasel hypersensitive sites 1, 2 and 3 of the human β-globin dominant control region direct position-independent expression

1990 • 186 citations

A Mouse Model for b-Thalassemia

1982 • 174 citations

A majority of mice show long-term expression of a human beta-globin gene after retrovirus transfer into hematopoietic stem cells.

1989 • 153 citations

Human Sickle Hemoglobin in Transgenic Mice

1990 • 131 citations

High-level beta-globin expression after retroviral transfer of locus activation region-containing human beta-globin gene derivatives into murine erythroleukemia cells.

1990 • 129 citations

A transgenic mouse model of sickle cell disorder

1990 • 105 citations

Modulation of gene expression in multiple hematopoietic cell lineages following retroviral vector gene transfer.

1987 • 89 citations

Retroviral gene transfer of human adenosine deaminase in murine hematopoietic cells: effect of selectable marker sequences on long-term expression

1991 • 77 citations

Monoclonal antibodies specific for globin chains

1983 • 67 citations

Expression of human alpha-globin and mouse/human hybrid beta-globin genes in murine hemopoietic stem cells transduced by recombinant retroviruses.

1990 • 23 citations