[14] The segmented paper method: DNA synthesis and mutagenesis by rapid microscale “shotgun gene synthesis”

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA

1982 • 764 citations

The gapped duplex DNA approach to oligonucleotide-directed mutation construction

1984 • 511 citations

An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides

1983 • 504 citations

Requirement of stereospecific alignments for initiation from the simian virus 40 early promoter

1986 • 336 citations

β-Cyanoethyl N,N-dialkylamino/N-morpholinomonochloro phosphoamidites, new phosphitylating agents facilitating ease of deprotection and work-up of synthesized oligonucleotides

1983 • 266 citations

Activation of heat-shock genes in eukaryotes

1985 • 263 citations

A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322

1981 • 260 citations

Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mers

1983 • 227 citations

Improvement Of Oligonucleotide-Directed Site-Specific Mutagenesis Using Double-Stranded Plasmid DNA

1984 • 189 citations

A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports

1983 • 181 citations

Rapid and reliable dideoxy sequencing of double-stranded DNA

1985 • 158 citations

Efficient site-directed mutagenesis by simultaneous use of two primers

1983 • 154 citations

The synthesis of oligodeoxyprimidines on a polymer support

1980 • 129 citations

Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl esters

1981 • 128 citations

Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale

1984 • 115 citations

Synthesis of oligonucleotides on cellulose by a phosphotriester method

1980 • 113 citations

Oligonucleotide-directed mutagenesis by microscale ‘shot-gun’ gene synthesis

1985 • 105 citations

Expression of bovine intestinal calcium binding protein from a synthetic gene in E. coli and characterization of the product

1986 • 83 citations

Filter disc supported oligonucleotide synthesis by the phosphite method

1984 • 34 citations

Site-directed mutagenesis

1982 • 30 citations

Sequencing of large double-stranded DNA using the dideoxy sequencing technique

1982 • 26 citations

In vitro mutagenesis

1982 • 14 citations

Chemical synthesis of genes

1982 • 10 citations

Computer management of oligonucleotide synthesis on cellulose filters

1986 • 2 citations

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