A human villin cDNA clone to investigate the differentiation of intestinal and kidney cells in vivo and in culture.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

1972 • 6,922 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination

1977 • 3,548 citations

A general method for isolation of high molecular weight DNA from eukaryotes

1976 • 2,827 citations

Human Tumor Cells in Vitro

1975 • 753 citations

Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.

1984 • 538 citations

Organization, Chemistry, and Assembly of the Cytoskeletal Apparatus of the Intestinal Brush Border

1985 • 525 citations

Villin is a major protein of the microvillus cystoskeleton which binds both G and F actin in a calcium-dependent manner

1980 • 444 citations

Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library.

1983 • 307 citations

Can villin be used to identify malignant and undifferentiated normal digestive epithelial cells?

1985 • 193 citations

F actin assembly modulated by villin: Ca++-dependent nucleation and capping of the barbed end

1981 • 167 citations

Regulation of actin polymerization by villin, a 95,000 dalton cytoskeletal component of intestinal brush borders

1980 • 149 citations

Characterization of an integral membrane glycoprotein associated with the microfilaments of pig intestinal microvilli.

1983 • 136 citations

Demonstration of at least two different actin-binding sites in villin, a calcium-regulated modulator of F-actin organization.

1981 • 115 citations

Calcium control of microfilaments: uncoupling of the F-actin-severing and -bundling activity of villin by limited proteolysis in vitro.

1981 • 102 citations

Solubilization and immune-detection of β-galactosidase hybrid proteins carrying foreign antigenic determinants

1983 • 102 citations

Villin associates with specific microfilamentous structures as seen by immunofluorescence microscopy on tissue sections and cells microinjected with villin

1981 • 75 citations

A convenient technique to compare the efficiency of promoters inEscherichia coli

1985 • 64 citations

A gelsolin-like Ca2+-dependent actin-binding domain in villin

1985 • 63 citations

Demonstration of three distinct calcium-binding sites in villin, a modulator of actin assembly.

1983 • 55 citations

Ligand-induced conformational changes in villin, a calcium-controlled actin-modulating protein.

1983 • 43 citations