Expression and isolation of antimicrobial small molecules from soil DNA libraries.

16S ribosomal DNA amplification for phylogenetic study

1991 • 11,010 citations

Prokaryotes: The unseen majority

1998 • 4,659 citations

DNA recovery from soils of diverse composition

1996 • 2,847 citations

A Molecular View of Microbial Diversity and the Biosphere

1997 • 2,747 citations

Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity

1998 • 2,361 citations

Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products

1998 • 1,855 citations

Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

1992 • 1,651 citations

16S rRNA sequences reveal numerous uncultured microorganisms in a natural community

1990 • 1,401 citations

Cloning and characterization of chi-1: a developmentally regulated member of a novel class of the ionotropic glutamate receptor family

1995 • 1,252 citations

Cloning the Soil Metagenome: a Strategy for Accessing the Genetic and Functional Diversity of Uncultured Microorganisms

2000 • 1,239 citations

Modular Organization of Genes Required for Complex Polyketide Biosynthesis

1991 • 883 citations

Indirubin, the active constituent of a Chinese antileukaemia medicine, inhibits cyclin-dependent kinases

1999 • 784 citations

Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast

1993 • 713 citations

Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction

1992 • 573 citations

Strategies for discovering drugs from previously unexplored natural products

2000 • 541 citations

Diversity and association of psychrophilic bacteria in Antarctic sea ice

1997 • 527 citations

Search and Discovery Strategies for Biotechnology: the Paradigm Shift

2000 • 475 citations

The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin.

1995 • 436 citations

Natural products as a resource for new drugs.

1996 • 365 citations

Bacterial diversity among small-subunit rRNA gene clones and cellular isolates from the same seawater sample

1997 • 363 citations

Molecular phylogeny of Archaea from soil

1997 • 349 citations

Comparison of phenotypic diversity and DNA heterogeneity in a population of soil bacteria

1990 • 327 citations

An Improved Approach for Construction of Bacterial Artificial Chromosome Libraries

1998 • 310 citations

The role of natural products in a modern drug discovery program

2000 • 228 citations

Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase

1991 • 215 citations

The Earth’s bounty: assessing and accessing soil microbial diversity

1999 • 204 citations

Characterization of a Yellowstone hot spring microbial community by 5S rRNA sequences

1985 • 192 citations

Antineoplastic agents from natural sources: achievements and future directions

2000 • 191 citations

Construction and Characterization of a Bovine Bacterial Artificial Chromosome Library

1995 • 190 citations

Highlight on the studies of anticancer drugs derived from plants in china

1994 • 170 citations

Indigo formation by microorganisms expressing styrene monooxygenase activity

1997 • 154 citations

Sequence analysis and functional characterization of the violacein biosynthetic pathway from Chromobacterium violaceum.

2000 • 143 citations

Artificial chromosomes for antibiotic-producing actinomycetes

2000 • 102 citations

Screening of natural products for antimicrobial agents

1990 • 102 citations

An effective method to extract DNA from environmental samples for polymerase chain reaction amplification and DNA fingerprint analysis

1994 • 97 citations

A Streptomyces avermitilis gene encoding a 4-hydroxyphenylpyruvic acid dioxygenase-like protein that directs the production of homogentisic acid and an ochronotic pigment in Escherichia coli

1994 • 87 citations

Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids

1995 • 79 citations

Construction of a 750-kb bacterial clone contig and restriction map in the region of human chromosome 21 containing the progressive myoclonus epilepsy gene.

1996 • 54 citations

Organization of the biosynthesis genes for the peptide antibiotic gramicidin S

1988 • 52 citations

A study of the contrasting effects of cyclosporine, FK 506, and rapamycin on the suppression of allograft rejection.

1990 • 52 citations

Broad-scale approaches to the determination of soil microbial community structure: Application of the community DNA hybridization technique

1996 • 45 citations

Identification of indigo-related pigments produced by Escherichia coli containing a cloned Rhodococcus gene

1992 • 42 citations

Retrofitting vectors for Escherichia coli-based artificial chromosomes (PACs and BACs) with markers for transfection studies.

1997 • 40 citations

Power unseen : how microbes rule the world

1994 • 34 citations

Purification, nucleotide sequence and some properties of a bifunctional isomerase/decarboxylase from the homoprotocatechuate degradative pathway of Escherichia coli C

1993 • 22 citations