Repair and evolution of nef in vivo modulates simian immunodeficiency virus virulence

PCR protocols — A guide to methods and applications

1990 • 12,897 citations

Importance of the nef gene for maintenance of high virus loads and for development of AIDS

1991 • 1,613 citations

Protective Effects of a Live Attenuated SIV Vaccine with a Deletion in the nef Gene

1992 • 1,025 citations

Absence of Intact nef Sequences in a Long-Term Survivor with Nonprogressive HIV-1 Infection

1995 • 1,008 citations

A reliable method for the recovery of DNA fragments from agarose and acrylamide gels

1981 • 916 citations

Serine phosphorylation-independent downregulation of cell-surface CD4 by nef

1991 • 760 citations

Temporal aspects of DNA and RNA synthesis during human immunodeficiency virus infection: evidence for differential gene expression

1989 • 574 citations

The human immunodeficiency virus-1 nef gene product: a positive factor for viral infection and replication in primary lymphocytes and macrophages.

1994 • 536 citations

HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling an oncogene product

1987 • 523 citations

Nef Protein of HIV-1 Is a Transcriptional Repressor of HIV-1 LTR

1988 • 366 citations

Persistent HIV antigenaemia and decline of HIV core antibodies associated with transition to AIDS.

1986 • 342 citations

Mutational analysis of the human immunodeficiency virus: the orf-B region down-regulates virus replication.

1987 • 310 citations

Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection.

1993 • 291 citations

Altered T cell activation and development in transgenic mice expressing the HIV-1 nef gene.

1993 • 282 citations

Lack of a negative influence on viral growth by the nef gene of human immunodeficiency virus type 1.

1989 • 266 citations

Human immunodeficiency virus type 1 negative factor is a transcriptional silencer.

1989 • 243 citations

Analysis of human immunodeficiency virus type 1 nef gene sequences present in vivo

1993 • 241 citations

Effects of mutations within the 3' orf open reading frame region of human T-cell lymphotropic virus type III (HTLV-III/LAV) on replication and cytopathogenicity

1986 • 236 citations

Nef protein of human immunodeficiency virus type 1: evidence against its role as a transcriptional inhibitor.

1989 • 227 citations

Expression of the type 1 human immunodeficiency virus Nef protein in T cells prevents antigen receptor-mediated induction of interleukin 2 mRNA.

1991 • 192 citations

Characterization of nef sequences in long-term survivors of human immunodeficiency virus type 1 infection

1995 • 191 citations

CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals.

1993 • 186 citations

Differential Effects of nef on HIV Replication: Implications for Viral Pathogenesis in the Host

1989 • 183 citations

Natural HIV-1 NEF accelerates virus replication in primary human lymphocytes

1992 • 158 citations

Evolution of human immunodeficiency virus type 1 nef and long terminal repeat sequences over 4 years in vivo and in vitro

1991 • 144 citations

Molecular and biological characterization of simian immunodeficiency virus macaque strain 32H proviral clones containing nef size variants

1994 • 127 citations

HIV with Multiple Gene Deletions as a Live Attenuated Vaccine for AIDS

1992 • 99 citations

The Role of Nef in the Replication Cycle of the Human and Simian Immunodeficiency Viruses

1994 • 88 citations

Upstream U3 sequences in simian immunodeficiency virus are selectively deleted in vivo in the absence of an intact nef gene

1994 • 80 citations

Decline of anti-p24 antibody precedes antigenaemia as correlate of prognosis in HIV-1 infection.

1987 • 77 citations

Human immunodeficiency virus type 1 Nef protein down-regulates transcription factors NF-kappa B and AP-1 in human T cells in vitro after T-cell receptor stimulation

1994 • 76 citations

Expression and biochemical characterization of human immunodeficiency virus type 1 nef gene product

1990 • 73 citations

An integration-defective U5 deletion mutant of human immunodeficiency virus type 1 reverts by eliminating additional long terminal repeat sequences

1994 • 62 citations

Human immunodeficiency virus type 1 nef quasispecies in pathological tissue

1992 • 52 citations

Biochemical and biological comparison of HIV-1 NEF and ras gene products

1991 • 52 citations

The 3′‐orf protein of human immunodeficiency virus shows structural homology with the phosphorylation domain of human interleukin‐2 receptor and the ATP‐binding site of the protein kinase family

1987 • 49 citations

Effect of nef Alleles on Replication of Human Immunodeficiency Virus Type 1

1993 • 45 citations

The role of upstream U3 sequences in the pathogenesis of simian immunodeficiency virus-induced AIDS in rhesus monkeys

1994 • 45 citations

Serine Phosphorylation-Independent Downregulation of Cell-Surface CD4 by nef

1991 • 38 citations

The development of PCR based assays for the detection and differentiation of simian immunodeficiency virus in vivo

1995 • 33 citations

The use of the polymerase chain reaction for the detection of Simian immunodeficiency virus in experimentally infected macaques

1990 • 32 citations

Biological and molecular characterization of human immunodeficiency virus (HIV-1BR) from the brain of a patient with progressive dementia

1989 • 30 citations

Purification and characterization of human immunodeficiency virus type 1 nef gene product expressed by a recombinant baculovirus

1991 • 28 citations

Nef Genes of SIV

1990 • 27 citations

Towards a Vaccine Against AIDS: Lessons from Simian Immunodeficiency Virus Vaccines

1994 • 23 citations

The production and purification of PCR-derived recombinant simian immunodeficiency virus p27 gag protein; its use in detecting serological and T-cell responses in macaques

1990 • 21 citations

On the HIV nef gene product

1992 • 15 citations

Reversion of a Polymerase-Defective Integrated HIV-1 Genome

1993 • 11 citations

Nef genes of SIV.

1990 • 8 citations

Adenovirus transformed monkey cell lines permissive to enteric adenovirus type 40

1990 • 6 citations