Mapping of deletions in the genome of the highly attenuated vaccinia virus MVA and their influence on virulence

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

A new technique for the assay of infectivity of human adenovirus 5 DNA

1973 • 9,740 citations

The complete DNA sequence of vaccinia virus

1990 • 847 citations

The purification of four strains of poxvirus

1962 • 553 citations

Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype

1985 • 447 citations

Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment

1986 • 269 citations

Deletion of the vaccinia virus growth factor gene reduces virus virulence

1988 • 238 citations

Passage history, properties and applicability of the attenuated vaccinia virus strain MVA

1975 • 230 citations

Conservation and Variation in Orthopoxvirus Genome Structure

1979 • 227 citations

Prevention of vaccinia virus infection in imiminodeficient mice by vector-directed IL-2 expression

1987 • 216 citations

Orthopoxvirus DNA: A comparison of restriction profiles and maps

1985 • 214 citations

MVA-Stufenimpfung gegen Pocken

1974 • 209 citations

Hemorrhage in lesions caused by cowpox virus is induced by a viral protein that is related to plasma protein inhibitors of serine proteases.

1986 • 207 citations

Vaccinia virus host range genes

1990 • 180 citations

Nucleotide sequence of the vaccinia virus hemagglutinin gene

1986 • 158 citations

Cloning and expression of foreign genes in vaccinia virus, using a host range selection system

1989 • 151 citations

Vaccinia Virus Encodes a Family of Genes with Homology to Serine Proteinase Inhibitors

1989 • 128 citations

Analysis of a large cluster of nonessential genes deleted from a vaccinia virus terminal transposition mutant

1988 • 115 citations

A cowpox virus gene required for multiplication in Chinese hamster ovary cells

1988 • 113 citations

Mapping and insertional mutagenesis of a vaccinia virus gene encoding a 13,800-Da secreted protein

1989 • 104 citations

Cloning and Characterization of the Gene Encoding the Major Protein of the A-type Inclusion Body of Cowpox Virus

1988 • 104 citations

Spontaneous deletions and duplications of sequences in the genome of cowpox virus.

1984 • 99 citations

Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus

1981 • 95 citations

Host range deletion mutant of vaccinia virus defective in human cells

1981 • 89 citations

Structural and functional analysis of orthopoxvirus epitopes with neutralizing monoclonal antibodies

1990 • 74 citations

A poxvirus-derived vector that directs high levels of expression of cloned genes in mammalian cells.

1988 • 69 citations

The white pock (μ) mutants of rabbit poxvirus. III. Terminal DNA sequence duplication and transposition in rabbit poxvirus

1980 • 67 citations

Deletion of a 9,000-base-pair segment of the vaccinia virus genome that encodes nonessential polypeptides

1981 • 66 citations

Intragenornic sequence transposition in monkeypox virus

1981 • 65 citations

Highly attenuated vaccinia virus mutants for the generation of safe recombinant viruses.

1989 • 58 citations

Isolation and characterization of attenuated mutants of vaccinia virus

1987 • 53 citations

Parameters of field inversion gel electrophoresis for the analysis of pox virus genomes

1988 • 51 citations

Partial deletion of the human host range gene in the attenuated vaccinia virus MVA

1989 • 47 citations

Mapping of a vaccinia host range sequence by insertion into the viral thymidine kinase gene

1985 • 46 citations

Vaccinia virus: a suitable vehicle for recombinant vaccines?

1989 • 46 citations

Generation of a dominant 8-MDa deletion at the left terminus of vaccinia virus DNA.

1985 • 36 citations

Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus

1976 • 24 citations

A single point mutation of Ala-25 to Asp in the 14,000-Mr envelope protein of vaccinia virus induces a size change that leads to the small plaque size phenotype of the virus

1989 • 23 citations

Vaccinia virus expression vector: a new tool for immunologists

1985 • 19 citations

Cross-reactivity among cowpox, ectromelia and vaccinia viruses with monoclonal antibodies recognizing distinct antigenic determinants in A-type inclusion bodies

1986 • 9 citations