Downregulation of CD1 Marks Acquisition of Functional Maturation of Human Thymocytes and Defines a Control Point in Late Stages of Human T Cell Development

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Depletion of CD4 ⁺ T Cells in Major Histocompatibility Complex Class II-Deficient Mice

1991 • 673 citations

Positive selection of lymphocytes

1994 • 563 citations

Serum-free medium for generation and propagation of functional human cytotoxic and helper T cell clones

1984 • 392 citations

Expression of the Bcl-2 protein in murine and human thymocytes and in peripheral T lymphocytes.

1993 • 286 citations

Ontogeny of human natural killer (NK) cells: fetal NK cells mediate cytolytic function and express cytoplasmic CD3 epsilon,delta proteins.

1992 • 263 citations

CD40 is functionally expressed on human thymic epithelial cells.

1992 • 261 citations

Bcl-2 is upregulated at the CD4+ CD8+ stage during positive selection and promotes thymocyte differentiation at several control Points

1994 • 252 citations

Evidence for a stochastic mechanism in the differentiation of mature subsets of T lymphocytes

1993 • 228 citations

CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection

1993 • 225 citations

Thymic selection in CD8 transgenic mice supports an instructive model for commitment to a CD4 or CD8 lineage

1991 • 209 citations

Regulated expression of human CD4 rescues helper T cell development in mice lacking expression of endogenous CD4.

1993 • 201 citations

CD34+CD38dim cells in the human thymus can differentiate into T, natural killer, and dendritic cells but are distinct from pluripotent stem cells

1996 • 200 citations

Precursors of CD3+CD4+CD8+ cells in the human thymus are defined by expression of CD34. Delineation of early events in human thymic development.

1993 • 165 citations

Expression of different CD8 isoforms on distinct human lymphocyte subpopulations

1991 • 159 citations

Peptides in positive and negative selection: A delicate balance

1994 • 153 citations

Engagement of the T-cell receptor during positive selection in the thymus down-regulates RAG-1 expression.

1992 • 152 citations

Development of the CD4 and CD8 lineage of T cells: instruction versus selection.

1991 • 144 citations

Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation.

1986 • 134 citations

Production, selection, and maturation of thymocytes with high surface density of TCR.

1994 • 113 citations

Regulation of RAG‐1 and CD69 expression in the thymus during positive and negative selection

1994 • 112 citations

Human natural killer cell committed thymocytes and their relation to the T cell lineage.

1993 • 107 citations

Bcl-2 expression during T-cell development: early loss and late return occur at specific stages of commitment to differentiation and survival.

1994 • 106 citations

Positive selection of T cells: rescue from programmed cell death and differentiation require continual engagement of the T cell receptor.

1995 • 104 citations

The development of T and non-T cell lineages from CD34+ human thymic precursors can be traced by the differential expression of CD44.

1995 • 101 citations

Characterization of distinct stages during the differentiation of human CD69+CD3+ thymocytes and identification of thymic emigrants.

1995 • 97 citations

Human CD34+ fetal liver stem cells differentiate to T cells in a mouse thymic microenvironment

1994 • 95 citations

IL-1, IL-4, and IFN-gamma differentially regulate cytokine production and cell surface molecule expression in cultured human thymic epithelial cells.

1991 • 87 citations

Thymic selection: two pathways to life and two to death

1994 • 83 citations

A role for the cytoplasmic tail of the β chain of CD8 in thymic selection

1994 • 70 citations

Positive selection of thymocytes involves sustained interactions with the thymic microenvironment.

1995 • 70 citations

Human bone marrow and umbilical cord blood cells generate CD4+ and CD8+ single-positive T cells in murine fetal thymus organ culture.

1993 • 68 citations

Subpopulations of mature murine thymocytes: Properties of CD4−CD8+ and CD4+CD8− thymocytes lacking the heat-stable antigen

1988 • 58 citations

The ligand for positive selection of T lymphocytes in the thymus

1994 • 56 citations

Polymerase chain reaction for detection of cytokine gene expression

1992 • 56 citations

The majority of postselection CD4+ single-positive thymocytes requires the thymus to produce long-lived, functional T cells.

1995 • 55 citations

Human thymocyte development in mouse organ cultures

1990 • 53 citations

Functional and phenotypic delineation of two subsets of CD4 single positive cells in the thymus

1990 • 51 citations

Differentiation to T helper cells in the thymus. Gradual acquisition of T helper cell function by CD3+CD4+ cells.

1995 • 35 citations

CD27 induction on thymocytes.

1990 • 35 citations

A Challenge to the Instructive Model of Positive Selection

1993 • 24 citations

Evidence for a selective and multi‐step model of T cell differentiation: CD4⁺CD8^low thymocytes selected by a transgenic T cell receptor on major histocompatibility complex class I molecules

1994 • 23 citations

In vitro differentiation from a pluripotent human CD4+CD8+ thymic cloned cell into four phenotypically distinct subsets.

1990 • 17 citations

A cross‐species functional interaction between the murine major histocompatibility complex class I α₃ domain and human CD8 revealed by peptide‐specific cytotoxic T lymphocytes

1992 • 14 citations

Establishment of human double-positive thymocyte clones.

1989 • 9 citations

Clonogenic potential of murine CD4+8+ thymocytes. Direct demonstration using a V beta 6-specific proliferative stimulus in Mlsa mice.

1989 • 7 citations

Identification of human pre-T/NK cell-associated genes.

1993 • 4 citations