Site-Specific Mutagenesis of cdc2⁺, a Cell Cycle Control Gene of the Fission Yeast Schizosaccharomyces pombe

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

1983 • 2,609 citations

Lambda replacement vectors carrying polylinker sequences

1983 • 1,809 citations

cdc25+ functions as an inducer in the mitotic control of fission yeast

1986 • 973 citations

Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template

1984 • 947 citations

Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe

1976 • 906 citations

Nucleotide sequence of rous sarcoma virus

1983 • 901 citations

Genetic control of cell size at cell division in yeast

1975 • 846 citations

Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast

1981 • 740 citations

The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation

1986 • 502 citations

Functionally homologous cell cycle control genes in budding and fission yeast

1982 • 410 citations

The erbB gene of avian erythroblastosis virus is a member of the src gene family

1983 • 377 citations

Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.

1985 • 370 citations

Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes

1984 • 354 citations

High-frequency transformation of the fission yeast Schizosaccharomyces pombe

1981 • 351 citations

REGULATORY GENES CONTROLLING MITOSIS IN THE FISSION YEAST SCHIZOSACCHAROMYCES POMBE

1980 • 350 citations

Direct evidence that oncogenic tyrosine kinases and cyclic AMP-dependent protein kinase have homologous ATP-binding sites

1984 • 345 citations

Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases

1984 • 313 citations

Replication and recombination functions associated with the yeast plasmid, 2μ circle

1980 • 296 citations

Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation

1982 • 242 citations

Nucleotide sequence of Fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses

1982 • 239 citations

Chapter 7 Physiological and Cytological Methods for Schizosaccharomyces pombe

1970 • 238 citations

Schizosaccharomyces pombe

1974 • 215 citations

Epistatic gene interactions in the control of division in fission yeast

1979 • 208 citations

Controls over the timing of DNA replication during the cell cycle of fission yeast

1977 • 204 citations

Affinity labeling of cAMP-dependent protein kinase with p-fluorosulfonylbenzoyl adenosine. Covalent modification of lysine 71.

1981 • 191 citations

[28] Quantitative two-dimensional gel electrophoresis of proteins

1983 • 188 citations

Complete amino acid sequence of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein kinase.

1981 • 188 citations

Mutants altered in the control co-ordinating cell division with cell growth in the fission yeast Schizosaccharomyces pombe

1978 • 150 citations

Mutagenesis of Fujinami Sarcoma Virus: Evidence that tyrosine phosphorylation of P130gag-fps modulates its biological activity

1984 • 142 citations

Phosphorylation of tyrosine-416 is not required for the transforming properties and kinase activity of pp60v-src

1983 • 138 citations

A mutation at the ATP-binding site of pp60v-src abolishes kinase activity, transformation, and tumorigenicity.

1985 • 126 citations

Isolation of cell size mutants of a fission yeast by a new selective method: characterization of mutants and implications for division control mechanisms

1981 • 110 citations

Transcription of the cdc2 cell cycle control gene of the fission yeast Schizosaccharomyces pombe

1986 • 87 citations

A lysine in the ATP-binding site of P130gag-fps is essential for protein-tyrosine kinase activity.

1986 • 80 citations

Lysine residue 121 in the proposed ATP-binding site of the v-mos protein is required for transformation.

1985 • 79 citations

Amino Acid Alterations Within a Highly Conserved Region of the Rous Sarcoma Virus src Gene Product pp60^src Inactivate Tyrosine Protein Kinase Activity

1984 • 75 citations

A Mutation at the ATP-Binding Site of pp60^v-src Abolishes Kinase Activity, Transformation, and Tumorigenicity

1985 • 39 citations

The fission yeast cell cycle control gene cdc2: structure of the cdc2 region

1985 • 28 citations

Changed Division Response Mutants Function as Allosuppressors

1984 • 13 citations

Sexual Differentiation Is Controlled by a Protein Kinase Encoded by the ran1+ Gene in Fission Yeast

1985 • 5 citations

Changes in phosphoprotein pattern in Schizosaccharomyces pombe

1985 • 3 citations