Peptide synthesis in aqueous–organic solvent mixtures with α‐chymotrypsin immobilized to tresyl chloride‐activated agarose

Enzyme structure and mechanism

1978 • 2,897 citations

Chemistry of the amino acids

1961 • 1,404 citations

Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixtures

1981 • 1,170 citations

Enzyme Handbook

1969 • 318 citations

Synthesis of peptide bonds by proteinases. Addition of organic cosolvents shifts peptide bond equilibriums toward synthesis

1978 • 245 citations

Immobilization of enzymes and affinity ligands to various hydroxyl group carrying supports using highly reactive sulfonyl chlorides

1981 • 231 citations

THE RÔLE OF SPECIFICITY IN THE ENZYMATIC SYNTHESIS OF PROTEINS

1937 • 192 citations

Demonstration of the acyl-enzyme mechanism for the hydrolysis of peptides and anilides by chymotrypsin

1973 • 191 citations

The Correlation of the pH (pD) Dependence and the Stepwise Mechanism of α-Chymotrypsin-Catalyzed Reactions

1964 • 170 citations

Proteinase‐Catalyzed Synthesis of Peptide Bonds

1982 • 150 citations

α-Chymotrypsin as the catalyst for peptide synthesis

1977 • 127 citations

Synthesis of an aspartame precursor by immobilized thermolysin in an organic solvent

1981 • 127 citations

Leaving group specificity in the chymotrypsin-catalyzed hydrolysis of peptides. Stereochemical interpretation

1973 • 125 citations

The interaction of tubulin and other proteins with structure-stabilizing solvents

1976 • 124 citations

Interaction of .beta.-lactoglobulin with solvent components in mixed water-organic solvent systems

1968 • 98 citations

Enzymatic peptide synthesis. Carboxypeptidase Y catalyzed formation of peptide bonds

1979 • 81 citations

Use of enzymes in peptide synthesis

1982 • 80 citations

Studies on conformation of soluble and immobilized enzymes using differential scanning calorimetry. 2. Specific activity and thermal stability of enzymes bound weakly and strongly to Sepharose CL 4B

1977 • 76 citations

The Denaturation by Urea and Guanidinium Chloride of Trypsin and N‐Acetylated‐Trypsin Derivatives Bound to Sephadex and Agarose

1973 • 67 citations

Enzyme-catalyzed peptide synthesis in biphasic aqueous-organic systems

1980 • 67 citations

Determination of the individual rate constants of α‐chymotrypsin‐catalyzed hydrolysis with the added nucleophilic agent, 1,4‐butanediol

1971 • 65 citations

Physicochemical investigation of the chymotrypsins. II. On the mechanism of dimerization of chymotrypsin

1957 • 63 citations

Enzymatic synthesis in biphasic aqueous-organic systems. II. Shift of ionic equilibria

1981 • 62 citations

α-Chymotrypsin-catalyzed hydrolysis of N-acetyl-L-tryptophan p-nitrophenyl ester in dimethyl sulfoxide at subzero temperatures

1973 • 62 citations

Proteases as catalysts for enzymic syntheses of opioid peptides.

1980 • 61 citations

Comparison of α-chymotrypsin and subtilisin BPN1: Size and specificity of the active site

1969 • 56 citations

Peptide Bond Synthesis Catalyzed by α-Chymotrypsin

1978 • 55 citations

Participation of an Acidic Group in the Chymotrypsin Catalysis***

1969 • 43 citations

The Application of Insolubilized α-Chymotrypsin to Kinetic Studies on the Effect of Aprotic Dipolar Organic Solvents

1974 • 40 citations

Protein Fractionation At Subzero Temperatures

1978 • 33 citations

X‐Ray Cryoenzymology

1981 • 29 citations