AutoDimer: a screening tool for primer-dimer and hairpin structures

Basic local alignment search tool

1990 • 87,358 citations

Primer3 on the WWW for General Users and for Biologist Programmers

2003 • 15,242 citations

A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics

1998 • 2,705 citations

Predicting DNA duplex stability from the base sequence.

1986 • 1,800 citations

Improved Nearest-Neighbor Parameters for Predicting DNA Duplex Stability

1996 • 829 citations

A computer program for choosing optimal oligonudeotides for filter hybridization, sequencing andin vitroamplification of DNA

1989 • 678 citations

Thermodynamics and NMR of Internal G·T Mismatches in DNA

1997 • 588 citations

Nearest-Neighbor Thermodynamics and NMR of DNA Sequences with Internal A·A, C·C, G·G, and T·T Mismatches

1999 • 560 citations

Improved Thermodynamic Parameters and Helix Initiation Factor to Predict Stability of DNA Duplexes

1996 • 458 citations

Nearest-Neighbor Thermodynamics of Internal A·C Mismatches in DNA: Sequence Dependence and pH Effects

1998 • 251 citations

Thermodynamic parameters for loop formation in RNA and DNA hairpin tetraloops

1992 • 241 citations

Nearest Neighbor Thermodynamic Parameters for Internal G·A Mismatches in DNA

1998 • 239 citations

Thermodynamics of internal C.T mismatches in DNA

1998 • 236 citations

A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers

2002 • 200 citations

Predicting sequence-dependent melting stability of short duplex DNA oligomers

1997 • 198 citations

Characterization of RNA hairpin loop stability

1988 • 197 citations

Discrimination of Primer 3′-Nucleotide Mismatch by Taq DNA Polymerase during Polymerase Chain Reaction

2000 • 172 citations

A multiplex allele-specific primer extension assay for forensically informative SNPs distributed throughout the mitochondrial genome

2004 • 150 citations

Selection of primers for polymerase chain reaction

1995 • 132 citations

Selecting signature oligonucleotides to identify organisms using DNA arrays

2002 • 120 citations

Studies of DNA dumbbells. I. Melting curves of 17 DNA dumbbells with different duplex stem sequences linked by T₄ endloops: Evaluation of the nearest‐neighbor stacking interactions in DNA

1992 • 110 citations

PRIMEGENS: robust and efficient design of gene-specific probes for microarray analysis

2002 • 94 citations

NMR solution structure of a DNA dodecamer containing single G*T mismatches

1998 • 93 citations

Multiplex PCR design strategy used for the simultaneous amplification of 10 Y chromosome short tandem repeat (STR) loci

2003 • 91 citations

Primer design for large scale sequencing

1998 • 88 citations

Efficient primer design algorithms

2001 • 88 citations

Primer-design for multiplexed genotyping

2003 • 82 citations

Melting studies of short DNA hairpins: Influence of loop sequence and adjoining base pair identity on hairpin thermodynamic stability

1999 • 72 citations

Primer Design Assistant (PDA): a web-based primer design tool

2003 • 63 citations

An Introduction to PCR Primer Design and Optimization of Amplification Reactions

2003 • 56 citations

Capillary electrophoresis as a tool for optimization of multiplex PCR reactions

2001 • 51 citations

Primer Design for PCR and Sequencing in High-Throughput Analysis of SNPs

2002 • 50 citations

Y-SNP Typing of U.S. African American and Caucasian Samples Using Allele-Specific Hybridization and Primer Extension

2004 • 48 citations

Predicting the success of primer extension genotyping assays using statistical modeling

2002 • 30 citations

Set of Novel Tools for PCR Primer Design

2001 • 25 citations

PCR Primer Design

2003 • 24 citations

A Greedy Algorithm for Minimizing the Number of Primers in Multiple PCR Experiments.

1999 • 22 citations

Sequence Context and Thermodynamic Stability of a Single Base Pair Mismatch in Short Deoxyoligonucleotide Duplexes

2001 • 19 citations

Computer Programs for PCR Primer Design and Analysis

2003 • 12 citations

DNA Sequence Context and Multiplex Hybridization Reactions: Melting Studies of Heteromorphic Duplex DNA Complexes

2002 • 11 citations