Identification and characterization of virK, a virulence‐associated large plasmid gene essential for intercellular spreading of Shigella flexneri

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin.

1989 • 724 citations

EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY

1964 • 628 citations

New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates

1979 • 463 citations

Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation

1987 • 327 citations

A genetic determinant required for continuous reinfection of adjacent cells on large plasmid in S. flexneri 2a

1986 • 312 citations

Experimental keratoconjunctivitis shigellosa.

1957 • 263 citations

Identification of a chromosomal gene controlling temperature-regulated expression of Shigella virulence.

1988 • 255 citations

Plaque formation by virulent Shigella flexneri

1985 • 253 citations

DNA supercoiling and environmental regulation of virulence gene expression in Shigella flexneri

1990 • 243 citations

virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence

1989 • 240 citations

Molecular alteration of the 140-megadalton plasmid associated with loss of virulence and Congo red binding activity in Shigella flexneri

1986 • 212 citations

A dual transcriptional activation system for the 230 kb plasmid genes coding for virulence‐associated antigens of Shigella flexneri

1989 • 199 citations

Virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in Shigella flexneri 2a

1988 • 195 citations

The two-component regulatory system ompR-envZ controls the virulence of Shigella flexneri

1990 • 186 citations

Temperature‐regulated expression of invasion genes in Shigella flexneri is controlled through the transcriptional activation of the virB gene on the large plasmid

1991 • 146 citations

Expression of four virulence antigens of Shigella flexneri is positively regulated at the transcriptional level by the 30 kilo Dalton virF protein

1988 • 118 citations

Intracellular spread of Shigella flexneri associated with the kcpA locus and a 140-kilodalton protein

1989 • 96 citations

A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis

1987 • 94 citations

Virulence‐associated chromosomal loci of Shigella flexneri identified by random Tn5 insertion mutagenesis

1991 • 79 citations

Isolation, characterization, and mapping of Tn5 insertions into the 140-megadalton invasion plasmid defective in the mouse Sereny test in Shigella flexneri 2a

1986 • 67 citations

Construction of a physical map of the chromosome of Shigella flexneri 2a and the direct assignment of nine virulence‐associated loci identified by Tn5 insertions

1991 • 59 citations

Transposon Tn5 target specificity: preference for insertion at G/C pairs.

1988 • 57 citations

IS50-mediated inverse transposition

1982 • 50 citations

The use of mice in the Sereny test as a virulence assay of shigellae and enteroinvasive Escherichia coli

1986 • 38 citations

Efficient Tn10 transposition into a DNA insertion hot spot in vivo requires the 5-methyl groups of symmetrically disposed thymines within the hot-spot consensus sequence.

1987 • 27 citations

Restoration of Virulence to a Strain of Shigella flexneri by Mating with Escherichia coli

1965 • 24 citations