PENICILLIN AND CEPHALOSPORIN BIOSYNTHETIC GENES: Structure, Organization, Regulation, and Evolution

Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host

1984 • 358 citations

Origin and evolution of organisms as deduced from 5S ribosomal RNA sequences.

1987 • 266 citations

The biosynthesis of penicillins and cephalosporins

1988 • 251 citations

The cluster of penicillin biosynthetic genes. Identification and characterization of the pcbAB gene encoding the alpha-aminoadipyl-cysteinyl-valine synthetase and linkage to the pcbC and penDE genes.

1990 • 240 citations

Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genes

1987 • 164 citations

Cloning and Expression of the Fungal Expandase/hydroxylase Gene Involved in Cephalosporin Biosynthesis

1987 • 164 citations

The Aspergillus nidulans npeA locus consists of three contiguous genes required for penicillin biosynthesis.

1990 • 140 citations

Cloning and Expression of a DNA Sequence Conferring Cephamycin C Production

1988 • 123 citations

The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum

1991 • 116 citations

δ -(L-α-Aminoadipyl)-L-cysteinyl-D-valine synthetase (ACV synthetase): a multifunctional enzyme with broad substrate specificity for the synthesis of penicillin and cephalosporin precursors

1987 • 114 citations

Cloning, sequence analysis and transcriptional study of the isopenicillin N synthase of Penicillium chrysogenum AS-P-78

1989 • 89 citations

The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus

1990 • 85 citations

Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities

1987 • 80 citations

The nucleotide sequences of the 5 S rRNAs of seven moids and a yeast and their use in studying ascomycete phylogeny

1984 • 64 citations

Cloning, characterization of the acyl-CoA : 6-amino penicillanic acid acyltransferase gene of Aspergillus nidulans and linkage to the isopenicillin N synthase gene

1990 • 63 citations

Evolution of isopenicillin N synthase genes may have involved horizontal gene transfer.

1990 • 62 citations

Lysine catabolism in Streptomyces spp. is primarily through cadaverine: beta-lactam producers also make alpha-aminoadipate

1989 • 61 citations

Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus

1989 • 58 citations

Purification of isopenicillin N synthetase from Streptomyces clavuligerus

1986 • 57 citations

Beta‐lactam biosynthetic genes

1989 • 49 citations

Two genes involved in penicillin biosynthesis are linked in a 5.1 kb SalI fragment in the genome of Penicillium chrysogenum

1989 • 48 citations

Molecular characterization and functional analysis in Aspergillus nidulans of the 5′-region of the Penicillium chrysogenum isopenicillin N synthetase gene

1991 • 37 citations

Antibiotics: Containing the Beta-Lactam Structure

1983 • 33 citations

Nucleotide sequence of the isopenicillin N synthase gene (pcbC) of the gram negativeFlavobacteriumsp. SC 12,154

1990 • 24 citations

Regulation of Isopenicillin N Synthetase (IPNS) Gene Expression in Acremonium Chrysogenum

1990 • 22 citations

The upstream region of the IPNS gene determines expression during secondary metabolism in Aspergillus nidulans

1990 • 21 citations

High level expression in Escherichia coli of a fungal gene under the control of strong promoters

1990 • 19 citations

High level expression inEscherichia coliof isopenicillin N synthase genes fromFlavobacteriumandStreptomyces, and recovery of active enzyme from inclusion bodies

1991 • 17 citations

Identification and expression of the ACV synthetase gene

1991 • 15 citations

Deleted Work

1955 • 0 citations