The ubiquitin‐mediated proteolytic pathway and mechanisms of energy‐dependent intracellular protein degradation

Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents.

1978 • 2,146 citations

Intracellular Protein Degradation in Mammalian and Bacterial Cells

1974 • 1,775 citations

The SOS regulatory system of Escherichia coli

1982 • 1,491 citations

Intracellular protein topogenesis

1980 • 1,378 citations

Protein turnover in mammalian tissues and in the whole body

1978 • 1,237 citations

Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown.

1983 • 1,105 citations

Intracellular Protein Degradation in Mammalian and Bacterial Cells: Part 2

1976 • 1,060 citations

Proteinases in mammalian cells and tissues

1978 • 981 citations

Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis.

1980 • 727 citations

Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells.

1975 • 691 citations

Mechanisms of Intracellular Protein Breakdown

1982 • 647 citations

A heat-stable polypeptide component of an ATP-dependent proteolytic system from reticulocytes

1978 • 622 citations

PROTEIN DEGRADATION IN CULTURED CELLS

1974 • 603 citations

A soluble ATP-dependent proteolytic system responsible for the degradation of abnormal proteins in reticulocytes.

1977 • 553 citations

Cleavage of the Escherichia coli lexA protein by the recA protease.

1980 • 550 citations

ATP-dependent conjugation of reticulocyte proteins with the polypeptide required for protein degradation.

1980 • 530 citations

Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.

1977 • 436 citations

Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation.

1982 • 413 citations

Carbohydrate moieties of glycoproteins a re-evaluation of their function

1982 • 413 citations

Ubiquitin is the ATP-dependent proteolysis factor I of rabbit reticulocytes.

1980 • 393 citations

The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity

1979 • 389 citations

Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the drosophila genome

1982 • 362 citations

E. coli recA protein-directed cleavage of phage λ repressor requires polynucleotide

1980 • 352 citations

Complete amino acid sequence of ubiquitin, an adenylate cyclase stimulating polypeptide probably universal in living cells

1975 • 350 citations

The product of the lon (capR) gene in Escherichia coli is the ATP-dependent protease, protease La.

1981 • 345 citations

Proteolytic cleavage of bacteriophage lambda repressor in induction.

1975 • 326 citations

Induction of autophagy by amino-acid deprivation in perfused rat liver

1977 • 323 citations

Studies on the Degradation of Tyrosine Aminotransferase in Hepatoma Cells in Culture

1971 • 319 citations

"Covalent affinity" purification of ubiquitin-activating enzyme.

1982 • 310 citations

A low resolution structure for the histone core of the nucleosome

1980 • 309 citations

Ubiquitin: Roles in protein modification and breakdown

1983 • 297 citations

Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells. Relationship to the breakdown of abnormal proteins.

1982 • 293 citations

Protein Turnover and Lysosome Function

1978 • 279 citations

Histone 2A, a heteromorphous family of eight protein species

1980 • 278 citations

The chemistry and biology of thymosin. I. Isolation, characterization, and biological activities of thymosin alpha1 and polypeptide beta1 from calf thymus.

1979 • 268 citations

Leupeptin, a Protease Inhibitor, Decreases Protein Degradation in Normal and Diseased Muscles

1978 • 267 citations

Attempts to Map a Process Evolution of Peptide Biosynthesis

1971 • 262 citations

Comparison of Nucleolar Proteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells by Two-Dimensional Polyacrylamide Gel Electrophoresis

1973 • 260 citations

ATP-dependent acidification of intact and disrupted lysosomes. Evidence for an ATP-driven proton pump.

1981 • 255 citations

Histone 2B can be modified by the attachment of ubiquitin

1980 • 245 citations

Characterization of the heat-stable polypeptide of the ATP-dependent proteolytic system from reticulocytes.

1980 • 243 citations

ATP hydrolysis-dependent protease activity of the lon (capR) protein of Escherichia coli K-12.

1981 • 242 citations

Cellular and molecular biology of erythrocytes

1974 • 239 citations

Control of Proteolysis

1980 • 238 citations

THE RELEASE OF LABELED AMINO ACIDS FROM THE PROTEINS OF RAT LIVER SLICES

1953 • 236 citations

Selective control of the degradation of normal and aberrant proteins in Reuber H35 hepatoma cells

1976 • 234 citations

Quantitative relationship between autophagy and proteolysis during graded amino acid deprivation in perfused rat liver.

1981 • 223 citations

Activation of the heat-stable polypeptide of the ATP-dependent proteolytic system.

1981 • 215 citations

Inhibition of protein degradation in isolated rat hepatocytes

1977 • 214 citations

Resolution of the ATP-dependent proteolytic system from reticulocytes: a component that interacts with ATP.

1979 • 210 citations

Direct evidence of importance of lysosomes in degradation of intracellular proteins

1975 • 209 citations

Deg phenotype of Escherichia coli lon mutants

1978 • 203 citations

Protein Degradation in Cultured Cells

1973 • 195 citations

Intracellular protein degradation

1993 • 192 citations

The chemistry and biology of thymosin. II. Amino acid sequence analysis of thymosin alpha1 and polypeptide beta1.

1979 • 191 citations

Amino-terminal sequence identity of ubiquitin and the nonhistone component of nuclear protein A24

1977 • 187 citations

Induction of SOS functions: Regulation of proteolytic activity of E. coil RecA protein by interaction with DNA and nucleoside triphosphate

1981 • 183 citations

Control of Enzyme Levels in Mammalian Tissues

1973 • 166 citations

ATP serves two distinct roles in protein degradation in reticulocytes, one requiring and one independent of ubiquitin.

1983 • 162 citations

The effect of protease inhibitors and decreased temperature on the degradation of different classes of proteins in cultured hepatocytes

1979 • 157 citations

Mutants of Escherichia coli with a Defect in the Degradation of Nonsense Fragments

1973 • 154 citations

Activity of the Escherichia coli recA-gene Product

1979 • 153 citations

Muscular Dystrophy: Inhibition of Degeneration in Vivo with Protease Inhibitors

1978 • 151 citations

Stimulation of ATP-dependent proteolysis requires ubiquitin with the COOH-terminal sequence Arg-Gly-Gly.

1981 • 150 citations

Identification and partial purification of an ATP-stimulated alkaline protease in rat liver.

1979 • 149 citations

E. coli contains eight soluble proteolytic activities, one being ATP dependent

1981 • 148 citations

Demonstration of an ATP-dependent, vanadate-sensitive endoprotease in the matrix of rat liver mitochondria.

1982 • 147 citations

Points of contact between histone H1 and the histone octamer.

1980 • 145 citations

Hemin inhibits ATP-dependent ubiquitin-dependent proteolysis: role of hemin in regulating ubiquitin conjugate degradation.

1981 • 141 citations

Protease La from Escherichia coli hydrolyzes ATP and proteins in a linked fashion.

1982 • 133 citations

Intracellular protein and nucleic acid turnover in resting yeast cells

1958 • 130 citations

Molecular conservation of 74 amino acid sequence of ubiquitin between cattle and man

1975 • 130 citations

Conjugation of ubiquitin to denatured hemoglobin is proportional to the rate of hemoglobin degradation in HeLa cells.

1982 • 128 citations

LYMPHOCYTE DIFFERENTIATION FROM PRECURSOR CELLS IN VITRO*

1975 • 127 citations

General characteristics of protein degradation in diabetes and starvation.

1978 • 125 citations

Requirement for protein synthesis in the regulation of protein breakdown in cultured hepatoma cells

1975 • 125 citations

Evidence for the involvement of H1 histone phosphorylation in chromosome condensation

1980 • 123 citations

Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.

1977 • 121 citations

Identification of the active amino acid residue of the polypeptide of ATP-dependent protein breakdown.

1981 • 117 citations

The effects of colchicine and cytochalasin B on uptake and degradation of asialo-glycoproteins in isolated rat hepatocytes

1979 • 115 citations

Ubiquitin ? protein conjugates

1981 • 113 citations

Effect of microtubular or translational inhibitors on general cell protein degradation. Evidence for a dual catabolic pathway

1977 • 110 citations

Presence of protein A24 in rat liver nucleosomes.

1977 • 108 citations

Changes in nucleolar proteins and their phosphorylation patterns during liver regeneration.

1975 • 107 citations

Observations on intracellular protein catabolism studied in vitro

1956 • 106 citations

Regulation of Synthesis of Non-Globin Proteins in Cell-free Extracts of Rabbit Reticulocytes

1973 • 105 citations

Oxidized Proteins in Erythrocytes Are Rapidly Degraded by the Adenosine Triphosphate-Dependent Proteolytic System

1982 • 104 citations

The selective degradation of injected proteins occurs principally in the cytosol rather than in lysosomes

1981 • 103 citations

Free ubiquitin is a non-histone protein of trout testis chromatin

1978 • 101 citations

Characteristics of the inhibition of hemoglobin synthesis in rabbit reticulocytes by threo-α-amino-β-chlorobutyric acid

1964 • 101 citations

THE INTRACELLULAR TURNOVER OF PROTEIN AND NUCLEIC ACIDS AND ITS ROLE IN BIOCHEMICAL DIFFERENTIATION

1960 • 100 citations

Identification and purification of the Lon ⁺ (capR ⁺ ) gene product, a DNA-binding protein

1981 • 95 citations

Isopeptidase: a novel eukaryotic enzyme that cleaves isopeptide bonds.

1982 • 92 citations

DNA stimulates ATP-dependent proteolysis and protein-dependent ATPase activity of protease La from Escherichia coli.

1982 • 92 citations

Limited Proteolysis in Microorganisms

1978 • 90 citations

Supercontracting Striated Muscle in a Vertebrate

1973 • 87 citations

Protein degradation is stimulated by ATP in extracts of Escherichia coli.

1979 • 86 citations

Osmotic alterations of the lysosomal system during rat liver perfusion: Reversible suppression by insulin and amino acids

1974 • 85 citations

Internalization of cytoplasmic protein by hepatic lysosomes in basal and deprivation-induced proteolytic states.

1981 • 84 citations

Control of protein degradation in reticulocytes and reticulocyte extracts by hemin.

1980 • 83 citations

Regulation of catabolism of microinjected ribonuclease A requires the amino-terminal 20 amino acids.

1983 • 81 citations

The lon Gene and Degradation of β-Galactosidase Nonsense Fragments

1973 • 81 citations

Two mutations that alter the regulatory activity of E. coli recA protein

1981 • 77 citations

A temperature-sensitive mutant of cultured mouse cells defective in chromosome condensation

1980 • 77 citations

Studies of the ATP-dependent proteolytic enzyme, protease La, from Escherichia coli.

1982 • 77 citations

Determination and Characteristics of Energy-Dependent Proteolysis in Rabbit Reticulocytes

1980 • 75 citations

Macrophage protein turnover. Evidence for lysosomal participation in basal proteolysis

1979 • 73 citations

Loss of ATP-dependent proteolysis with maturation of reticulocytes and erythrocytes.

1982 • 72 citations

Isolation, characterization, and amino acid sequence of a ubiquitin-like protein from insect eggs.

1982 • 72 citations

Chromatin conjugate protein A24 is cleaved and ubiquitin is lost during chicken erythropoiesis.

1980 • 72 citations

A high molecular weight protease in liver cytosol.

1979 • 67 citations

A mouse temperature-sensitive mutant defective in H1 histone phosphorylation is defective in deoxyribonucleic acid synthesis and chromosome condensation

1981 • 64 citations

Concerning a possible mechanism for selective capture of cytoplasmic proteins by lysosomes

1975 • 64 citations

Histone 1 is proximal to histone 2A and to A24.

1979 • 63 citations

Proteolysis in homogenates of perfused rat liver: Responses to insulin, glucagon and amino acids

1973 • 62 citations

Inhibition of basal and deprivation-induced proteolysis by leupeptin and pepstatin in perfused rat liver and heart

1979 • 61 citations

Protein A24 lyase activity in nucleoli of thioacetamide-treated rat liver releases histone 2A and ubiquitin from conjugated protein A24

1981 • 57 citations

High-resolution fractionation of nucleosomes: minor particles, "whiskers," and separation of mononucleosomes containing and lacking A24 semihistone.

1980 • 56 citations

SOME ASPECTS OF THE INTRACELLULAR BREAKDOWN OF EXOGENOUS AND ENDOGENOUS PROTEINS

1978 • 53 citations

Hyperdegradation of proteins in Escherichia coli rho mutants.

1979 • 50 citations

Site of premature termination of late transcription of simian virus 40 DNA: enhancement by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole.

1982 • 47 citations

Properties of abnormal proteins degraded rapidly in reticulocytes. Intracellular aggregation of the globin molecules prior to hydrolysis.

1981 • 45 citations

Decrease in uH2A (protein A24) of a mouse temperature‐sensitive mutant

1983 • 44 citations

ATP-stimulated endoprotease is associated with the cell membrane of E. coli

1981 • 42 citations

Structure of nucleosome core particles containing uH2A (A24)

1981 • 40 citations

MODE OF DEGRADATION OF ABNORMAL GLOBIN CHAINS IN RABBIT RETICULOCYTES

1978 • 39 citations

Cellular and SV40 Chromatin: Replication, Segregation, Ubiqiritination, Nuclease-hypersensitive Sites, HMG-containing Nueleosomes, and Heterochromatin-specific Protein

1983 • 38 citations

Localization of products of endogenous proteolysis in lysosomes of perfused rat liver

1974 • 38 citations

Degradation of microinjected methylated and unmethylated proteins in hepatoma tissue culture cells.

1983 • 35 citations

Studies of the protein encoded by the lon mutation, capR9, in Escherichia coli. A labile form of the ATP-dependent protease La that inhibits the wild type protease.

1983 • 35 citations

Proteolysis in Bacterial Sporulation

1980 • 33 citations

Degradation of abnormal proteins in intact mouse reticulocytes: accumulation of intermediates in the presence of bestatin.

1979 • 33 citations

Protein A24 lyase is an isopeptidase

1981 • 32 citations

Disappearance of a basic chromosomal protein from cells of a mouse temperature-sensitive mutant defective in histone phosphorylation

1980 • 31 citations

Rapid degradation of puromycyl peptides in hepatoma cells and reticulocytes

1974 • 28 citations

Role of Non-Protein Nitrogen in the Synthesis of Hæmoglobin in the Reticulocyte in vitro

1956 • 27 citations

SELECTIVE DEGRADATION OF ABNORMAL PROTEINS IN ANIMAL AND BACTERIAL CELLS

1978 • 21 citations

A novel proteolytic activity apparently initiating degradation of beta-galactosidase nonsense fragments in in vitro extracts of Escherichia coli.

1983 • 14 citations

Limited proteolysis. Early steps in the processing of large premature termination fragments of beta-galactosidase in Escherichia coli.

1981 • 13 citations

A Temperature-Sensitive Mutant and the Nature of G2-Mitosis Transition

1982 • 7 citations