Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GC clamp

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels

1977 • 1,607 citations

Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

1989 • 1,295 citations

pEMBL: a new family of single stranded plasmids

1983 • 1,231 citations

[31] Detection and localization of single base changes by denaturing gradient gel electrophoresis

1987 • 944 citations

Single-stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering

1986 • 816 citations

An Improved Method for Prenatal Diagnosis of Genetic Diseases by Analysis of Amplified DNA Sequences

1987 • 811 citations

Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis

1985 • 606 citations

The nucleotide sequence of the human β-globin gene

1980 • 502 citations

[30] Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis

1987 • 491 citations

Base-base mismatches. Thermodynamics of double helix formation for dCA₃XA₃G + dCT₃YT₃G (X, Y = A,C,G,D

1985 • 426 citations

Detection of single base substitutions in total genomic DNA

1985 • 375 citations

An improved filamentous helper phage for generating single-stranded plasmid DNA

1986 • 348 citations

DNA Diagnostics—Molecular Techniques and Automation

1988 • 286 citations

Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis

1985 • 252 citations

Molecular basis and prenatal diagnosis of beta-thalassemia

1988 • 248 citations

Sequence-Determined DNA Separations

1984 • 210 citations

Molecular basis and prenatal diagnosis of beta-thalassemia

1988 • 197 citations

Mismatches in DNA double strands: thermodynamic parameters and their correlation to repair efficiencies

1986 • 160 citations

Two-dimensional DNA fingerprinting of human individuals.

1989 • 80 citations

Acceleration of DNA renaturation rates

1975 • 78 citations

Alterations in DNA helix stability due to base modifications can be evaluated using denaturing gradient gel electrophoresis

1987 • 64 citations

DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions

1977 • 64 citations

Searching for Gene Defects by Denaturing Gradient Gel Electrophoresis

1986 • 54 citations

Detection of base mutations in genomic DNA using denaturing gradient gel electrophoresis (DGGE) followed by transfer and hybridization with gene-specific probes

1988 • 44 citations

Identification of an amber nonsense mutation in the rosy516 gene by germline transformation of an amber suppressor tRNA gene.

1988 • 42 citations

Detection of human DNA polymorphisms with a simplified denaturing gradient gel electrophoresis technique.

1987 • 42 citations

Polymerase Chain Reaction

1990 • 15 citations

The use of denaturing gradient gel electrophoresis to screen for DNA sequence polymorphisms in the human factor VIII gene

1989 • 8 citations