Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry

An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database

1994 • 6,412 citations

Empirical Statistical Model To Estimate the Accuracy of Peptide Identifications Made by MS/MS and Database Search

2002 • 4,851 citations

Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

1999 • 4,699 citations

The SWISS-PROT protein sequence data bank and its supplement TrEMBL in 1999

1999 • 3,156 citations

The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000

2000 • 2,834 citations

Essentials of Glycobiology

1999 • 2,495 citations

Direct analysis of protein complexes using mass spectrometry

1999 • 2,242 citations

PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization

1999 • 2,159 citations

Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae

2002 • 1,646 citations

The Yeast Nuclear Pore Complex

2000 • 1,383 citations

Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds

2002 • 1,353 citations

Accurate quantitation of protein expression and site-specific phosphorylation

1999 • 1,014 citations

Essentials of Glycobiology

2000 • 966 citations

Large-Scale Proteomic Analysis of the Human Spliceosome

2002 • 911 citations

Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry

2001 • 891 citations

Comprehensive proteomic analysis of the human spliceosome

2002 • 883 citations

The detection and classification of membrane-spanning proteins

1985 • 854 citations

Characterization of glycoproteins and their associated oligosaccharides through the use of endoglycosidases

1989 • 774 citations

Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome

2001 • 771 citations

A systematic approach to the analysis of protein phosphorylation

2001 • 720 citations

Structural requirements of N-glycosylation of proteins. Studies with proline peptides as conformational probes

1983 • 606 citations

The LNCaP cell line--a new model for studies on human prostatic carcinoma.

1980 • 531 citations

Better prediction of protein cellular localization sites with the k nearest neighbors classifier.

1997 • 441 citations

ProteinN-Glycosylation along the Secretory Pathway: Relationship to Organelle Topography and Function, Protein Quality Control, and Cell Interactions

2002 • 384 citations

Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine

1992 • 346 citations

Monoclonal antibody therapy of human cancer: Taking the HER2 protooncogene to the clinic

1991 • 296 citations

Periodate Oxidation of Carbohydrates

1956 • 257 citations

Protein Glycosylation and Diseases: Blood and Urinary Oligosaccharides as Markers for Diagnosis and Therapeutic Monitoring

2000 • 228 citations

Proteomic profiling of mechanistically distinct enzyme classes using a common chemotype

2002 • 224 citations

Approaching complete peroxisome characterization by gas-phase fractionation

2002 • 189 citations

Quantitative Proteomic Analysis Using a MALDI Quadrupole Time-of-Flight Mass Spectrometer

2001 • 179 citations

Congenital disorders of glycosylation: genetic model systems lead the way

2001 • 168 citations

Crystal structure of cleaved human α1-antichymotrypsin at 2.7 å resolution and its comparison with other serpins

1991 • 166 citations

Update and perspectives on congenital disorders of glycosylation

2001 • 155 citations

Proteome analysis using selective incorporation of isotopically labeled amino acids

2000 • 143 citations

Enhancing the intensities of lysine-terminated tryptic peptide ions in matrix-assisted laser desorption/ionization mass spectrometry

2000 • 105 citations

A method for determination of N-glycosylation sites in glycoproteins by collision-induced dissociation analysis in fast atom bombardment mass spectrometry: Identification of the positions of carbohydrate-linked asparagine in recombinant α-amylase by treatment with peptide-N-glycosidase F in 18O-labeled water

1992 • 104 citations

Simplification of complex peptide mixtures for proteomic analysis: Reversible biotinylation of cysteinyl peptides

2000 • 99 citations

The rat liver mitochondrial proteins

2002 • 96 citations

The complete amino acid sequence of the A-chain of human plasma alpha 2HS-glycoprotein.

1986 • 88 citations

Biocytin hydrazide—A selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology

1988 • 81 citations

γ‐Phosphate‐linked ATP‐Sepharose for the affinity purification of protein kinases

1993 • 76 citations

Abundance Ratio-Dependent Proteomic Analysis by Mass Spectrometry

2003 • 66 citations

Analysis by matrix assisted laser desorption/ ionisation-time of flight mass spectrometry of the post-translational modifications of α1-antitrypsin isoforms separated by two- dimensional polyacrylamide gel electrophoresis

2001 • 41 citations

Identification of nuclei associated proteins by 2D-gel electrophoresis and mass spectrometry

2001 • 39 citations