A cluster of fourteen genes from enteropathogenic Escherichia coli is sufficient for the biogenesis of a type IV pilus

Molecular cloning: A laboratory manual

1990 • 85,659 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

A simple method for displaying the hydropathic character of a protein

1982 • 22,471 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

The complete general secretory pathway in gram-negative bacteria

1993 • 1,668 citations

Escherichia coli that Cause Diarrhea: Enterotoxigenic, Enteropathogenic, Enteroinvasive, Enterohemorrhagic, and Enteroadherent

1987 • 1,279 citations

Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin.

1987 • 1,041 citations

An adhesive factor found in strains ofEscherichia coli belonging to the traditional infantile enteropathogenic serotypes

1979 • 639 citations

An Inducible Bundle-Forming Pilus of Enteropathogenic Escherichia coli

1991 • 610 citations

Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells

1984 • 600 citations

STRUCTURE-FUNCTION AND BIOGENESIS OF THE TYPE IV PILI

1993 • 494 citations

The Diarrheal Response of Humans to Some Classic Serotypes of Enteropathogenic Escherichia coli is Dependent on a Plasmid Encoding an Enteroadhesiveness Factor

1985 • 471 citations

Pilus and nonpilus bacterial adhesins: Assembly and function in cell recognition

1993 • 421 citations

Common components in the assembly of type 4 fimbriae, DNA transfer systems, filamentous phage and protein‐secretion apparatus: a general system for the formation of surface‐associated protein complexes

1993 • 370 citations

A Clinicopathologic Study of Enterocyte Adherent Escherichia coli: A Cause of Protracted Diarrhea in Infants

1982 • 350 citations

Localization of the receptor-binding protein adhesin at the tip of the bacterial pilus

1987 • 343 citations

P pili in uropathogenic E. coli are composite fibres with distinct fibrillar adhesive tips

1992 • 338 citations

Plasmid-Mediated Adhesion in Enteropathogenic Escherichia coli

1983 • 326 citations

Neisseria PilC protein identified as type-4 pilus tip-located adhesin

1995 • 314 citations

The Rieske protein from Paracoccus denitrificans is inserted into the cytoplasmic membrane by the twin‐arginine translocase

2006 • 308 citations

A plasmid‐encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence

1992 • 288 citations

Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria

1991 • 273 citations

A plasmid-encoded regulatory region activates chromosomal eaeA expression in enteropathogenic Escherichia coli

1995 • 270 citations

Structural Basis of Pilus Subunit Recognition by the PaPd Chaperone

1993 • 215 citations

Outer-membrane PapC molecular usher discriminately recognizes periplasmic chaperone-pilus subunit complexes.

1993 • 212 citations

Pilus‐facilitated adherence of Neisseria meningitidis to human epithelial and endothelial cells: modulation of adherence phenotype occurs concurrently with changes in primary amino acid sequence and the glycosylation status of pilin

1993 • 193 citations

Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium Myxococcus xanthus

1994 • 192 citations

PilS and PilR, a two‐component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa

1993 • 192 citations

Sequence similarities between the RP4 Tra2 and the Ti VirB region strongly support the conjugation model for T-DNA transfer.

1992 • 161 citations

Construction and analysis of TnphoA mutants of enteropathogenic Escherichia coli unable to invade HEp-2 cells

1990 • 158 citations

The binding of Pseudomonas aeruginosa pili to glycosphingolipids is a tip‐associated event involving the C‐terminal region of the structural pilin subunit

1994 • 156 citations

Initiation of assembly and association of the structural elements of a bacterial pilus depend on two specialized tip proteins.

1993 • 148 citations

The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY

1993 • 134 citations

Distribution of the Bundle-Forming Pilus Structural Gene (bfpA) among Enteropathogenic Escherichia coli

1993 • 123 citations

Vibrio cholerae O395 tcpA pilin gene sequence and comparison of predicted protein structural features to those of type 4 pilins

1990 • 113 citations

Characterization of the Pseudomonas aeruginosa pilus adhesin: confirmation that the pilin structural protein subunit contains a human epithelial cell-binding domain

1989 • 103 citations

Processing of TCP pilin by TcpJ typifies a common step intrinsic to a newly recognized pathway of extracellular protein secretion by gram-negative bacteria.

1991 • 103 citations

Plasmid-mediated virulence in Salmonella dublin demonstrated by use of a Tn5-oriT construct

1985 • 102 citations

Localized adherence by enteropathogenic Escherichia coli is an inducible phenotype associated with the expression of new outer membrane proteins.

1991 • 98 citations

Phage Assembly: a Paradigm for Bacterial Virulence Factor Export?

1994 • 92 citations

Cloning and characterization of the bundle‐forming pilin gene of enteropathogenic Escherichia coli and its distribution in Salmonella serotypes

1993 • 90 citations

Processing and methylation of PulG, a pilin‐like component of the general secretory pathway of Klebsiella oxytoca

1993 • 87 citations

Genetic organization and sequence of the promoter-distal region of the tcp gene cluster of Vibrio cholerae

1993 • 83 citations

Characterization of plasmids encoding the adherence factor of enteropathogenic Escherichia coli

1987 • 82 citations

A plasmid-encoded prepilin peptidase gene from enteropathogenic Escherichia coli

1994 • 65 citations

Cloning and sequencing of Vibrio cholerae mannose‐sensitive haemagglutinin pilin gene: localization of mshA within a cluster of type 4 pilin genes

1994 • 65 citations

Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili

1995 • 58 citations

Carbon dioxide regulated secretion of the EaeB protein of enteropathogenicEscherichia coli

1995 • 43 citations

Escherichia coli contains a set of genes homologous to those involved in protein secretion, DNA uptake and the assembly of type-4 fimbriae in other bacteria

1994 • 34 citations

Genetic characterization of mannose-sensitive hemagglutinin (MSHA)-negative mutants of Vibrio cholerae derived by Tn5 mutagenesis

1994 • 24 citations

Cloning of the Genes That Control Formation of the Fimbrial Colonization Factor Antigen III (CFA/III) from an Enterotoxigenic Escherichia coli

1993 • 13 citations

The Toxin-Coregulated Pilus: Biogenesis and Function

2014 • 12 citations

Attachment to and Entry of Microorganisms into the Body

1995 • 2 citations