Physical characterisation of the Escherichia coli B gene encoding nitroreductase and its over-expression in Escherichia coli K12

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Improved Estimation of Secondary Structure in Ribonucleic Acids

1973 • 2,302 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

The pMTL nic− cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing

1988 • 418 citations

The bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)—I

1992 • 211 citations

A sensitive method for the detection of mutagenic nitroarenes: construction of nitroreductase-overproducing derivatives of Salmonella typhimurium strains TA98 and TA100

1989 • 174 citations

A new cytotoxic, DNA interstrand crosslinking agent, 5-(aziridin-1-yl)-4-hydroxylamino-2-nitrobenzamide, is formed from 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB 1954) by a nitroreductase enzyme in walker carcinoma cells

1988 • 167 citations

Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence

1992 • 125 citations

Identification of the gene encoding the major NAD(P)H-flavin oxidoreductase of the bioluminescent bacterium Vibrio fischeri ATCC 7744

1994 • 124 citations

Molecular cloning of the Pseudomonas carboxypeptidase G2 gene and its expression in Escherichia coli and Pseudomonas putida

1983 • 122 citations

Humanized antibodies for therapy

1991 • 110 citations

The bioactivation of CB 1954 and its use as a prodrug in antibody-directed enzyme prodrug therapy (ADEPT)

1993 • 107 citations

The bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)—II

1992 • 104 citations

Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae

1991 • 81 citations

Bioactivation of CB 1954: Reaction of the active 4-hydroxylamino derivative with thioesters to form the ultimate DNA-DNA interstrand crosslinking species

1991 • 76 citations

Hypoxia-selective antitumor agents. 5. Synthesis of water-soluble nitroaniline mustards with selective cytotoxicity for hypoxic mammalian cells

1992 • 75 citations

Nucleotide sequence ofSalmonella typhimuriumnitroreductase gene

1990 • 65 citations

CB 1954 (2,4-dinitro-5-aziridinyl benzamide) becomes a DNA interstrand crosslinking agent in Walker tumour cells

1986 • 62 citations

Nitrofuratoin-resistant mutants of Escherichia coli: Isolation and mapping

1984 • 21 citations

Molecular cloning and nucleotide sequence determination of the Bacillus stearothermophilus NCA 1503 superoxide dismutase gene and its overexpression in Escherichia coli

1991 • 15 citations

The toxicity and mutagenicity of the anti-tumour drug 5-aziridino-2, 4-dinitrobenzamide (CB1954) is greatly reduced in a nitroreductase-deficient strain of E. coli

1987 • 14 citations