Mutagenesis of the putative alpha-helical domain of the Vpr protein of human immunodeficiency virus type 1: effect on stability and virion incorporation.

Molecular cloning: A laboratory manual

1990 • 85,659 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

Importance of the nef gene for maintenance of high virus loads and for development of AIDS

1991 • 1,613 citations

Use of Helical Wheels to Represent the Structures of Proteins and to Identify Segments with Helical Potential

1967 • 1,178 citations

The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in nondividing host cells.

1994 • 845 citations

Pathogenesis of human immunodeficiency virus infection

1993 • 835 citations

Amphipathic helix motif: Classes and properties

1990 • 634 citations

Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.

1987 • 409 citations

Incorporation of Vpr into human immunodeficiency virus type 1 virions: requirement for the p6 region of gag and mutational analysis

1993 • 368 citations

Human immunodeficiency virus vpr product is a virion-associated regulatory protein

1990 • 363 citations

An infectious molecular clone of an unusual macrophage-tropic and highly cytopathic strain of human immunodeficiency virus type 1

1992 • 353 citations

Human immunodeficiency virus type 1 viral protein R localization in infected cells and virions

1993 • 275 citations

Identification of HIV-1 vpr product and function.

1990 • 251 citations

Distinct Effects in Primary Macrophages and Lymphocytes of the Human Immunodeficiency Virus Type 1 Accessory Genes vpr, vpu, and nef: Mutational Analysis of a Primary HIV-1 Isolate

1994 • 237 citations

Rev is necessary for translation but not cytoplasmic accumulation of HIV-1 vif, vpr, and env/vpu 2 RNAs.

1991 • 232 citations

Evolution of the primate lentiviruses: evidence from vpx and vpr.

1992 • 232 citations

Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus

1993 • 220 citations

Induction of cell differentiation by human immunodeficiency virus 1 vpr

1993 • 214 citations

Human immunodeficiency virus as a prototypic complex retrovirus

1991 • 197 citations

Vpu protein of human immunodeficiency virus type 1 enhances the release of capsids produced by gag gene constructs of widely divergent retroviruses.

1993 • 189 citations

Mutational analysis of the human immunodeficiency virus vpr open reading frame

1989 • 183 citations

Targeting of pre-ornithine transcarbamylase to mitochondria: Definition of critical regions and residues in the leader peptide

1986 • 172 citations

The human immunodeficiency virus type 2 vpr gene is essential for productive infection of human macrophages.

1990 • 159 citations

Human Immunodeficiency Virus: The Eighth Gene

1987 • 143 citations

Isolation and Characterization of a Novel Protein (X-ORF Product) from SIV and HIV-2

1988 • 141 citations

Biochemical mechanism of HIV-I Vpr function. Specific interaction with a cellular protein.

1994 • 131 citations

Human Immunodeficiency VirusvprGene Encodes a Virion-Associated Protein

1990 • 126 citations

Human retroviruses and aids, 1992

1992 • 119 citations

Dual regulation of silent and productive infection in monocytes by distinct human immunodeficiency virus type 1 determinants

1992 • 117 citations

Requirement of the Pr55gag precursor for incorporation of the Vpr product into human immunodeficiency virus type 1 viral particles

1994 • 113 citations

Viral protein R of human immunodeficiency virus types 1 and 2 is dispensable for replication and cytopathogenicity in lymphoid cells

1989 • 103 citations

Rev activates expression of the human immunodeficiency virus type 1 vif and vpr gene products

1991 • 102 citations

Antisense phosphorothioate oligodeoxynucleotides targeted to the vpr gene inhibit human immunodeficiency virus type 1 replication in primary human macrophages

1993 • 101 citations

Studies of a Putative Amphipathic Helix in the N-Terminus of Poliovirus Protein 2C

1994 • 89 citations

Mutational Analysis of the Nucleic Acid-Binding 17 kDa Phosphoprotein of Potato Leafroll Luteovirus Identifies an Amphipathic α-Helix as the Domain for Protein/Protein Interactions

1993 • 85 citations

Identification of Residues in the N-Terminal Acidic Domain of HIV-1 Vpr Essential for Virion Incorporation

1995 • 83 citations

Human immunodeficiency virus type 1 Vpu protein induces degradation of chimeric envelope glycoproteins bearing the cytoplasmic and anchor domains of CD4: role of the cytoplasmic domain in Vpu-induced degradation in the endoplasmic reticulum

1993 • 81 citations

Intracellular Transport and Virion Incorporation of vpx Requires Interaction with Other Virus Type-Specific Components

1993 • 59 citations

Genetic evidence that the Tat proteins of human immunodeficiency virus types 1 and 2 can multimerize in the eukaryotic cell nucleus

1993 • 57 citations

Statistical and functional analyses of viral and cellular proteins with N-terminal amphipathic alpha-helices with large hydrophobic moments: importance to macromolecular recognition and organelle targeting

1988 • 26 citations

HIV-2 Viral Protein X Association with the Gag p27 Capsid Protein

1994 • 26 citations

Context-dependent role of human immunodeficiency virus type 1 auxiliary genes in the establishment of chronic virus producers

1993 • 25 citations