Targeted alterations in yeast artificial chromosomes for inter-species gene transfer

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Genomic sequencing.

1984 • 8,343 citations

Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins

1989 • 3,219 citations

Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells

1987 • 2,368 citations

Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome Vectors

1987 • 1,517 citations

Yeast transformation: a model system for the study of recombination.

1981 • 1,491 citations

Replacement of chromosome segments with altered DNA sequences constructed in vitro.

1979 • 779 citations

An efficient salt-chloroform extraction of DNA from blood and tissues.

1989 • 638 citations

Base sequence studies of 300 nucleotide renatured repeated human DNA clones

1981 • 605 citations

Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments

1980 • 402 citations

Isolation of Single-Copy Human Genes from a Library of Yeast Artificial Chromosome Clones

1989 • 316 citations

Structure and function of the yeast URA3 gene: expression in Escherichia coli

1984 • 311 citations

Germ-Line Transmission of a c- abl Mutation Produced by Targeted Gene Disruption in ES Cells

1989 • 285 citations

Yeast: an Experimental Organism for Modern Biology

1988 • 284 citations

Transformation of yeast spheroplasts without cell fusion

1987 • 266 citations

Chromosomal Region of the Cystic Fibrosis Gene in Yeast Artificial Chromosomes: A Model for Human Genome Mapping

1990 • 238 citations

Somatic hypermutation of immunoglobulin kappa may depend on sequences 3′ of C kappa and occurs on passenger transgenes.

1991 • 132 citations

Yeast artificial chromosomes: tools for mapping and analysis of complex genomes

1990 • 110 citations

Complex pattern of immunoglobulin mu gene expression in normal and transgenic mice: nonoverlapping regulatory sequences govern distinct tissue specificities.

1991 • 99 citations

Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes.

1990 • 93 citations

Modification and transfer into an embryonal carcinoma cell line of a 360-kilobase human-derived yeast artificial chromosome.

1990 • 91 citations

Transfer of a yeast artificial chromosome carrying human DNA from Saccharomyces cerevisiae into mammalian cells.

1990 • 90 citations

Construction of LYS2 cartridges for use in genetic manipulations of Saccharomyces cerevisiae

1986 • 87 citations

Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes.

1991 • 58 citations

Rescue of end fragments of yeast artificial chromosomes by homologous recombination in yeast

1991 • 58 citations

Molecular complementation of a collagen mutation in mammalian cells using yeast artificial chromosomes.

1992 • 43 citations

Vectors for inserting selectable markers in vector arms and human DNA inserts of yeast artificial chromosomes (YACs)

1991 • 37 citations

Stable integration and expression in mouse cells of yeast artificial chromosomes harboring human genes.

1991 • 36 citations

The effect of DNA concentration on mobility i pulsed field gel electrophoresis

1992 • 33 citations

Modification and Transfer into an Embryonal Carcinoma Cell Line of a 360-Kilobase Human-Derived Yeast Artificial Chromosome

1990 • 29 citations

Transfer of yeast artificial chromosomes from yeast to mammalian cells

1991 • 23 citations

Generation of a nested series of interstitial deletions in yeast artificial chromosomes carrying human DNA.

1991 • 18 citations

Characterization and expression of the human leukocyte-common antigen (CD45) gene contained in yeast artificial chromosomes

1991 • 15 citations

Integrative selection of human chromosome-specific yeast artificial chromosomes.

1991 • 9 citations