A cell culture system that enhances mononuclear cell IgE synthesis induced by recombinant human interleukin-4

Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

1968 • 5,084 citations

IgE production by normal human lymphocytes is induced by interleukin 4 and suppressed by interferons gamma and alpha and prostaglandin E2.

1988 • 939 citations

IL-4 is an essential factor for the IgE synthesis induced in vitro by human T cell clones and their supernatants.

1988 • 713 citations

Serum-free medium for generation and propagation of functional human cytotoxic and helper T cell clones

1984 • 392 citations

Measurement of IgE in normal and allergic serum by radioimmunoassay.

1971 • 335 citations

Induction of human IgE synthesis requires interleukin 4 and T/B cell interactions involving the T cell receptor/CD3 complex and MHC class II antigens.

1989 • 331 citations

IgE production by normal human B cells induced by alloreactive T cell clones is mediated by IL-4 and suppressed by IFN-gamma.

1988 • 315 citations

Interleukin 5 enhances interleukin 4‐induced IgE production by normal human B cells. The role of soluble CD23 antigen

1988 • 265 citations

Recombinant interleukin 4 promotes the growth of human T cells.

1987 • 252 citations

B cell growth-promoting activity of recombinant human interleukin 4.

1987 • 211 citations

IgE secretion by Epstein-Barr virus-infected purified human B lymphocytes is stimulated by interleukin 4 and suppressed by interferon gamma.

1989 • 167 citations

Suppressor cells in the regulation of the immune response.

1977 • 144 citations

Human IgE Biosynthesis in Vitro: Studies with Atopic and Normal Blood Mononuclear Cells and Subpopulations

1979 • 120 citations

Human IgE synthesis in vitro: a reassessment.

1981 • 70 citations

In vitro synthesis of IgE by human peripheral blood leucocytes. III. Release of pre-formed antibody.

1983 • 46 citations

Lack of pokeweed mitogen-induced IgE formation in vitro by human peripheral blood mononuclear cells: detection of cross-reacting idiotypic determinants on polyclonal Ig by antibodies to a single IgE myeloma protein.

1983 • 43 citations

Effect of recombinant human interleukin 4 on spontaneous in vitro human IgE synthesis^*

1988 • 41 citations

Growth of human malignant lymphoid cell lines in serum-free medium

1983 • 29 citations

Variability of IgE protein measurement in cell-culture supernatants: Results from a multicenter collaborative study

1986 • 26 citations

Induction of immunoglobulin-producing human peripheral blood lymphocytes in serum-free medium

1982 • 17 citations

Application of a serum-free medium in the growth and differentiation of human peripheral blood lymphocytes

1986 • 12 citations

Workshop on measurement of in vitro IgE synthesis and regulation of IgE synthesis

1986 • 12 citations

In vitro Synthesis of IgE by Human Peripheral Blood Leucocytes

1985 • 12 citations

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