nifU Gene Product from Azotobacter vinelandii Is a Homodimer That Contains Two Identical [2Fe-2S] Clusters

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION

1949 • 16,078 citations

Cysteine desulfurase activity indicates a role for NIFS in metallocluster biosynthesis.

1993 • 579 citations

EPR signal intensity and powder shapes: A reexamination

1975 • 502 citations

The Redox Potential of Dithionite and SO⁻₂ from Equilibrium Reactions with Flavodoxins, Methyl Viologen and Hydrogen plus Hydrogenase

1978 • 484 citations

Nitrogenase and biological nitrogen fixation

1994 • 419 citations

Determination of Iron with o-Phenanthroline: A Spectrophotometric Study

1938 • 413 citations

Mechanism for the Desulfurization of L-Cysteine Catalyzed by the nifS Gene Product

1994 • 394 citations

Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii

1989 • 367 citations

Biochemical and genetic analysis of the nifUSVWZM cluster from Azotobacter vinelandii

1989 • 313 citations

Iron—Sulfur Clusters in Enzymes: Themes and Variations

1992 • 247 citations

Purification and characterization of the Rieske iron-sulfur protein from Thermus thermophilus. Evidence for a [2Fe-2S] cluster having non-cysteine ligands.

1984 • 247 citations

Isolation and characterisation of the crnA-niiA-niaD gene cluster for nitrate assimilation in Aspergillus nidulans

1990 • 220 citations

Nitrogenase metalloclusters: structures, organization, and synthesis

1993 • 217 citations

Human ferrochelatase is an iron-sulfur protein

1994 • 208 citations

Electron-nuclear double resonance spectroscopy of nitrogen-15-enriched phthalate dioxygenase from Pseudomonas cepacia proves that two histidines are coordinated to the [2Fe-2S] Rieske-type clusters

1989 • 196 citations

Nitrogen fixation (nif) genes of the cyanobacterium Anabaena species strain PCC 7120

1989 • 151 citations

Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB–cysG region of the Escherichia coli K‐12 chromosome

1990 • 135 citations

Optimal conditions for transformation of Azotobacter vinelandii

1979 • 129 citations

Magnetization curves of haemoproteins measured by low-temperature magnetic-circular-dichroism spectroscopy

1980 • 119 citations

Structure of the [2Fe-2S]ferredoxin I from the blue-green Alga Aphanothece sacrum at 2·2 Å resolution

1990 • 111 citations

Potential ligands to the [2Fe-2S] Rieske cluster of the cytochrome bc1 complex of Rhodobacter capsulatus probed by site-directed mutagenesis

1992 • 107 citations

Effect of magnesium adenosine 5'-triphosphate on the accessibility of the iron of clostridial azoferredoxin, a component of nitrogenase

1974 • 91 citations

A ligand-field model to describe a new class of 2Fe-2S clusters in proteins and their synthetic analogues

1985 • 89 citations

Molecular structure of the oxidized, recombinant, heterocyst [2Fe-2S] (iron-sulfur) ferredoxin from Anabaena 7120 determined to 1.7-.ANG. resolution

1993 • 86 citations

Reactions with the oxidized iron protein of Azotobacter vinelandii nitrogenase: formation of a 2Fe center

1984 • 84 citations

Resonance Raman and magnetic circular dichroism studies of reduced [2Fe-2S] proteins.

1992 • 84 citations

Fe2S2 protein resonance Raman spectra revisited: structural variations among adrenodoxin, ferredoxin, and red paramagnetic protein

1989 • 84 citations

Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al

1993 • 76 citations

[9] Variable-temperature magnetic circular dichroism

1993 • 73 citations

Site-directed mutagenesis of conserved cysteine residues in Escherichia coli fumarate reductase: modification of the spectroscopic and electrochemical properties of the [2Fe-2S] cluster.

1990 • 71 citations

Isolation, sequencing, and mutagenesis of the nifF gene encoding flavodoxin from Azotobacter vinelandii.

1988 • 71 citations

An electrochemical method for measuring redox potentials of low potential proteins by microcoulometry at controlled potentials

1979 • 69 citations

Optical, EPR, and 1H NMR Spectroscopy of Serine-Ligated [2Fe-2S] Ferredoxins Produced by Site-Directed Mutagenesis of Cysteine Residues in Recombinant Anabaena 7120 Vegetative Ferredoxin

1994 • 66 citations

Resonance Raman studies of Rieske-type proteins

1992 • 63 citations

Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa

1993 • 62 citations

Electrochemical characterization of the iron-molybdenum cofactor from Azotobacter vinelandii nitrogenase

1985 • 60 citations

Site-directed mutagenesis of the nitrogenase MoFe protein of Azotobacter vinelandii

1987 • 59 citations

Amino acid sequence of the [2Fe-2S] ferredoxin from Clostridium pasteurianum

1986 • 57 citations

Vibrational spectra and normal mode analysis for [2Fe-2S] protein analogs using sulfur-34, iron-54 and deuterium substitution: coupling of iron-sulfur stretching and sulfur-carbon-carbon bending modes

1989 • 57 citations

Comparative organization of nitrogen fixation-specific genes from Azotobacter vinelandii and Klebsiella pneumoniae: DNA sequence of the nifUSV genes

1987 • 56 citations

Evidence that conserved residues Cys‐62 and Cys‐154 within the Azotobacter vinelandii nitrogenase MoFe protein α‐subunit are essential for nitrogenase activity but conserved residues His‐83 and Cys‐88 are not

1990 • 41 citations

Mutated Forms of a [2Fe-2S] Ferredoxin with Serine Ligands to the Iron-Sulfur Cluster

1993 • 37 citations

Identification of free and [Fe2S2]-bound cysteine residues of adrenodoxin.

1988 • 33 citations

A Simple Anaerobic Cell for Low-Temperature Raman Spectroscopy

1988 • 27 citations

Properties of the iron-sulfur center in the 25-kilodalton subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans.

1994 • 27 citations

Identification of 2Fe-2S cysteine ligands in putidaredoxin

1990 • 26 citations

The modular structure of NifU proteins

1994 • 26 citations

Variable-Temperature Magnetic Circular Dichroism Studies of Metalloproteins

1988 • 15 citations

Electron-spin-resonance studies of the NADH-dependent nitrite reductase from Escherichia coli K12

1982 • 14 citations