Cohesin-dockerin recognition in cellulosome assembly: Experiment versus hypothesis

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Molecular cloning: A laboratory manual

1990 • 85,659 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

Spectroscopic Determination of Tryptophan and Tyrosine in Proteins^*

1967 • 3,423 citations

Metal chelate affinity chromatography, a new approach to protein fractionation

1975 • 2,050 citations

Short protocols in molecular biology

1996 • 1,701 citations

Site-directed mutagenesis of virtually any plasmid by eliminating a unique site

1992 • 1,089 citations

Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families

1991 • 868 citations

Cellulose, cellulases and cellulosomes

1998 • 592 citations

Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum

1983 • 559 citations

Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.

1991 • 464 citations

The cellulosome — A treasure-trove for biotechnology

1994 • 457 citations

Cellulase families revealed by hydrophobic cluster analysi

1989 • 390 citations

Cellulosomes—Structure and Ultrastructure

1998 • 336 citations

Refined crystal structure of troponin C from turkey skeletal muscle at 2·0 Å resolution

1988 • 335 citations

The cellulosome concept as an efficient microbial strategy for the degradation of insoluble polysaccharides

1999 • 334 citations

[14] Protein biotinylation

1990 • 252 citations

The Cellulosome of Clostridium thermocellum

1988 • 231 citations

Species-specificity of the cohesin-dockerin interaction betweenClostridium thermocellum andClostridium cellulolyticum: Prediction of specificity determinants of the dockerin domain

1997 • 214 citations

The Cellulosome: An Exocellular, Multiprotein Complex Specialized in Cellulose Degradation

1996 • 206 citations

Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity

1988 • 172 citations

Interaction of the duplicated segment carried by Clostridium thermocellum cellulases with cellulosome components

1991 • 152 citations

Cellulose-Binding Domains: Classification and Properties

1996 • 138 citations

Expression, purification and subunit‐binding properties of cohesins 2 and 3 of the Clostridium thermocellum cellulosome

1995 • 138 citations

Genetics, biochemistry and ecology of cellulose degradation

1999 • 134 citations

Isolation and properties of a major cellobiohydrolase from the cellulosome of Clostridium thermocellum

1991 • 130 citations

A cohesin domain from Clostridium thermocellum: the crystal structure provides new insights into cellulosome assembly

1997 • 115 citations

Calcium-binding affinity and calcium-enhanced activity of Clostridium thermocellum endoglucanase D

1990 • 113 citations

Characterization of endoglucanase A from Clostridium cellulolyticum

1991 • 101 citations

Cloning and DNA sequence of the gene coding for Clostridium thermocellum cellulase Ss (CelS), a major cellulosome component

1993 • 99 citations

Recognition specificity of the duplicated segments present in Clostridium thermocellum endoglucanase CelD and in the cellulosome-integrating protein CipA

1994 • 99 citations

Cellulosome from Clostridium cellulolyticum: Molecular Study of the Dockerin/Cohesin Interaction

1999 • 96 citations

Spatial separation of protein domains is not necessary for catalytic activity or substrate binding in a xylanase

1990 • 95 citations

Interaction between the endoglucanase CelA and the scaffolding protein CipC of the Clostridium cellulolyticum cellulosome

1996 • 87 citations

The crystal structure of a type I cohesin domain at 1.7 Å resolution 1 1Edited by D. Rees

1997 • 86 citations

Interactions of the CelS binding ligand with various receptor domains of the Clostridium thermocellum cellulosomal scaffolding protein, CipA

1996 • 68 citations

Structural Role of Calcium for the Organization of the Cellulosome of Clostridium thermocellum

1996 • 44 citations

Cloning and expression of theClostridium thermocellum celS gene inEscherichia coli

1994 • 39 citations

Isolation of Cry1Ab protein mutants ofBacillus thuringiensisby a highly efficient PCR site-directed mutagenesis system

1996 • 33 citations

Involvement of Both Dockerin Subdomains in Assembly of the Clostridium thermocellum Cellulosome

1998 • 29 citations

Deleted Work

1955 • 0 citations