Functional and immunochemical characterization of a mutant of Escherichia coli energy uncoupled for lactose transport

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

MUTANTS OF ESCHERICHIA COLI REQUIRING METHIONINE OR VITAMIN B ₁₂

1950 • 2,926 citations

[13] Bacterial Membranes

1971 • 653 citations

Sequence of the lactose permease gene

1980 • 501 citations

Purification and reconstitution of functional lactose carrier from Escherichia coli.

1981 • 313 citations

Structure of the lac carrier protein of Escherichia coli.

1983 • 299 citations

The Role of the Phosphoenolpyruvate-phosphotransferase System in the Transport of Sugars by Isolated Membrane Preparations of Escherichia coli

1968 • 270 citations

Quantitative measurements of membrane potential in Escherichia coli

1980 • 226 citations

Mechanisms of Active Transport in Isolated Membrane Vesicles

1971 • 182 citations

TheLac carrier protein inEscherichia coli

1983 • 179 citations

Localization of D-lactate dehydrogenase in native and reconstituted Escherichia coli membrane vesicles

1975 • 167 citations

Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 1. Effect of pH on efflux, exchange, and counterflow

1979 • 165 citations

[72] Transport in isolated bacterial membrane vesicles

1974 • 165 citations

Electrochemical proton gradient in inverted membrane vesicles from Escherichia coli

1980 • 154 citations

Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 2. Effect of imposed .DELTA..PSI., .DELTA.pH, and .DELTA..lovin..mu.H+

1979 • 143 citations

[75] The use of cyanine dyes for the determination of membrane potentials in cells, organelles, and vesicles

1979 • 117 citations

Active transport in membrane vesicles from Escherichia coli: the electrochemical proton gradient alters the distribution of the lac carrier between two different kinetic states

1980 • 115 citations

Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes.

1984 • 114 citations

Peptide-specific antibody locates the COOH terminus of the lactose carrier of Escherichia coli on the cytoplasmic side of the plasma membrane.

1983 • 103 citations

Preparation, characterization, and properties of monoclonal antibodies against the lac carrier protein from Escherichia coli.

1982 • 101 citations

Monoclonal antibodies against the lac carrier protein from Escherichia coli. 2. Binding studies with membrane vesicles and proteoliposomes reconstituted with purified lac carrier protein

1984 • 96 citations

Monoclonal antibodies against the lac carrier protein from Escherichia coli. 1. Functional studies

1984 • 86 citations

Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. I. Effect of pH and imposed membrane potential on efflux, exchange, and counterflow

1983 • 85 citations

Site-directed mutagenesis of cys148 in the lac carrier protein of Escherichia coli

1984 • 85 citations

Purified reconstituted lac carrier protein from Escherichia coli is fully functional.

1984 • 83 citations

Topology of the lac carrier protein in the membrane of Escherichia coli.

1983 • 79 citations

Sidedness of native membrane vesicles of Escherichia coli and orientation of the reconstituted lactose :H⁺ carrier

1984 • 79 citations

Mechanism of lactose translocation in proteoliposomes reconstituted with lac carrier protein purified from Escherichia coli. II. Deuterium solvent isotope effects

1983 • 77 citations

Equilibrium between two forms of the lac carrier protein in energized and nonenergized membrane vesicles from Escherichia coli

1976 • 73 citations

[74] The use of flow dialysis for determinations of ΔpH and active transport

1979 • 71 citations

Lactose carrier protein of Escherichia coli. Transport and binding of 2'-(N-dansyl)aminoethyl .beta.-D-thiogalactopyranoside and p-nitrophenyl .alpha.-D-galactopyranoside

1979 • 62 citations

Energy Coupling in the Lactose Transport System of Escherichia coli

1970 • 59 citations

Isolation, genetic analysis, and characterization of Escherichia coli mutants with defects in the lacY gene

1977 • 58 citations

A mutant of escherichia coli K12 energy-uncoupled for lactose transport

1972 • 52 citations

Direct measurement of lactose/proton symport in Escherichia coli membrane vesicles: further evidence for the involvement of a histidine residue(s)

1982 • 50 citations

Lactose permease: a carrier on the move

1983 • 45 citations

Lactose transport in Escherichia coli cells Dependence of kinetic parameters on the transmembrane electrical potential difference

1981 • 40 citations

Functional molecular weight of the lac carrier protein from Escherichia coli as studied by radiation inactivation analysis.

1984 • 37 citations

Lifetime and rotational relaxation time of dansylgalactoside bound to the lac carrier protein

1975 • 36 citations