Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli

Basic local alignment search tool

1990 • 87,358 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

New families in the classification of glycosyl hydrolases based on amino acid sequence similarities

1993 • 1,995 citations

A SUBMICRODETERMINATION OF GLUCOSE

1949 • 1,578 citations

A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides

1983 • 1,143 citations

A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm

1993 • 897 citations

Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum

1983 • 559 citations

Preparation of crystalline, amorphous, and dyed cellulase substrates

1988 • 323 citations

Cellulosome: a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities

1983 • 227 citations

AMPLIFICATION OF FLANKING SEQUENCES BY INVERSE PCR

1990 • 214 citations

Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal S_L‐protein reveals an unusual degree of internal homology

1993 • 205 citations

Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum

1988 • 186 citations

Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A.

1992 • 169 citations

Clostridium cellulolyticum sp. nov., a Cellulolytic, Mesophilic: Species from Decayed Grass

1984 • 153 citations

Crystal structure of the catalytic domain of a bacterial cellulase belonging to family 5

1995 • 152 citations

Interaction of the duplicated segment carried by Clostridium thermocellum cellulases with cellulosome components

1991 • 152 citations

Organization of a Clostridium thermocellum gene cluster encoding the cellulosomal scaffolding protein CipA and a protein possibly involved in attachment of the cellulosome to the cell surface

1993 • 149 citations

Isolation and properties of a major cellobiohydrolase from the cellulosome of Clostridium thermocellum

1991 • 130 citations

Characterization of endoglucanase A from Clostridium cellulolyticum

1991 • 101 citations

Cloning and DNA sequence of the gene coding for Clostridium thermocellum cellulase Ss (CelS), a major cellulosome component

1993 • 99 citations

Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component

1995 • 98 citations

Cloning of a nitrogen fixation (nif) gene cluster of Azospirillum brasilense

1982 • 92 citations

Metabolism and Solubilization of Cellulose by Clostridium cellulolyticum H10

1985 • 89 citations

Metabolism of glucose and cellobiose by cellulolytic mesophilic Clostridium sp. strain H10

1983 • 82 citations

Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum

1992 • 80 citations

Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi

1995 • 73 citations

Properties of a thermoactive β-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum expressed in Escherichia coli

1991 • 72 citations

Involvement of separate domains of the cellulosomal protein S1 of Clostridium thermocellum in binding to cellulose and in anchoring of catalytic subunits to the cellulosome

1992 • 69 citations

Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for a beta-mannanase from the extremely thermophilic bacterium "Caldocellum saccharolyticum"

1991 • 67 citations

Purification and properties of a novel type of exo‐1,4‐β‐glucanase (Avicelase II) from the cellulolytic thermophile Clostridium stercorarium

1991 • 67 citations

Purification and characterization of endoglucanase C from Clostridium cellulolyticum

1993 • 65 citations

The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome.

1995 • 64 citations

Characterization of exoglucanase and synergistic hydrolysis of cellulose inClostridium stercorarium

1983 • 61 citations

Sequence analysis of the Clostridium cellulolyticum endoglucanase-A-encoding gene, celCCA

1989 • 58 citations

A Bacteroides ruminicola 1,4-beta-D-endoglucanase is encoded in two reading frames

1991 • 49 citations

The catalytic domain of endoglucanase A from Clostridium cellulolyticum: effects of arginine 79 and histidine 122 mutations on catalysis

1992 • 44 citations

Cellulase Ss (CelS) is synonymous with the major cellobiohydrolase (subunit S8) from the cellulosome ofClostridium thermocellum

1993 • 42 citations

Cloning and expression of theClostridium thermocellum celS gene inEscherichia coli

1994 • 39 citations

Nucleotide sequences of the celB gene encoding endo-1,4-β-β-glucanase-2, ORF1 and ORF2 forming a putative cellulase gene cluster of Clostridium josui

1993 • 31 citations

Partial purification and characterization of the cellulases from clostridium cellulolyticum h10

1991 • 27 citations

Nucleotide sequence analysis of the endoglucanase-encoding gene, celCCD, of Clostridium cellulolyticum

1991 • 27 citations

Expression and mutation of soybean β‐amylase in Escherichia coli

1993 • 24 citations

Stereochemical Course of Hydrolysis Catalyzed by Cellulomonas fimi CenE, a Member of a New Family of β-1,4-Glucanases

1994 • 24 citations

Cloning and expression of the Clostridium thermocellum celS gene in Escherichia coli

1994 • 18 citations

Biochemistry and genetics of cellulose degradation. FEMS symposium no. 43

1990 • 18 citations

Crystallization and Preliminary X-ray Analysis of the Catalytic Domain of Endoglucanase from Clostridium cellulolyticum

1993 • 17 citations

Precise localization of an overproduced periplasmic protein in Escherichia coli: use of double immuno‐gold labelling

1987 • 16 citations