One-step affinity purification of bacterially produced proteins by means of the “Strep tag” and immobilized recombinant core streptavidin

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

[6] Use of T7 RNA polymerase to direct expression of cloned genes

1990 • 6,681 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

Avidin

1975 • 1,425 citations

[5] Avidin and streptavidin

1990 • 1,005 citations

Information content of binding sites on nucleotide sequences

1986 • 953 citations

Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea

1986 • 926 citations

Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid.

1989 • 744 citations

Measurement of protein by spectrophotometry at 205 nm

1974 • 742 citations

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment

1993 • 288 citations

Molecular cloning and nucleotide sequence of the streptavidin gene

1986 • 186 citations

Engineering proteins for purification

1990 • 165 citations

Characterization and crystallization of core streptavidin.

1987 • 146 citations

Expression of a cloned streptavidin gene in Escherichia coli.

1990 • 143 citations

Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity

1992 • 119 citations

The pRSET family of T7 promoter expression vectors for Escherichia coli

1993 • 113 citations

Crystal structure and ligand binding studies of a screened peptide complexed with streptavidin

1992 • 97 citations

[8] Isolation and properties of streptavidin

1990 • 92 citations

Postsecretory modifications of streptavidin

1989 • 88 citations

The azurin gene from Pseudomonas aeruginosa codes for a pre‐protein with a signal peptide

1987 • 65 citations

The bilin‐binding protein of Pieris brassicae

1994 • 59 citations

Cloning and expression of the gene encoding the soluble cytochrome b₅₆₂ of Escherichia coli

1991 • 53 citations

Avidin. 5. Quenching of fluorescence by dinitrophenyl groups

1964 • 42 citations

A spin label study of egg white avidin.

1975 • 38 citations

Functional Expression of the Uncomplexed Serum Retinol-binding Protein in Escherichia coli

1993 • 30 citations

Isolation and characterization of highly purified streptavidin obtained in a two-step purification procedure from Streptomyces avidinii grown in a synthetic medium

1988 • 24 citations

Synthesis of a (desSer1 Ile29 Leu89) chicken cystatin gene, expression in E. coli as fusion protein and its isolation

1989 • 18 citations

Production of streptavidin in a synthetic medium

1988 • 11 citations