Directed Mutagenesis of Chloroplast Ribulose-1,5-bisphosphate Carboxylase/Oxygenase

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

Regulation of Soybean Net Photosynthetic CO₂ Fixation by the Interaction of CO₂, O₂, and Ribulose 1,5-Diphosphate Carboxylase

1974 • 660 citations

Species variation in the specificity of ribulose biphosphate carboxylase/oxygenase

1981 • 517 citations

Development of the particle inflow gun for DNA delivery to plant cells

1992 • 501 citations

The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA

1981 • 420 citations

STRUCTURE, FUNCTION, REGULATION, AND ASSEMBLY OF <scp>D</scp>-RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE

1994 • 348 citations

Crystallographic analysis of ribulose 1,5-bisphosphate carboxylase from spinach at 2·4 Å resolution

1990 • 312 citations

Interaction of ribulosebisphosphate carboxylase/oxygenase with transition-state analogs

1980 • 278 citations

A simple and rapid method for the selection of oligodeoxynucleotide-directed mutants

1988 • 274 citations

Chloroplast gene sequence for the large subunit of ribulose bisphosphatecarboxylase of maize

1980 • 269 citations

Sequence of the chloroplast DNA region of Chlamydomonas reinhardii containing the gene of the large subunit of ribulose bisphosphate carboxylase and parts of its flanking genes

1982 • 255 citations

Molecular cloning and sequence analysis of the cyanobacterial gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase

1983 • 156 citations

Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes

1981 • 150 citations

High Substrate Specificity Factor Ribulose Bisphosphate Carboxylase/Oxygenase from Eukaryotic Marine Algae and Properties of Recombinant Cyanobacterial Rubisco Containing "Algal" Residue Modifications

1994 • 146 citations

A Sensitive Assay Procedure for Simultaneous Determination of Ribulose-1,5-bisphosphate Carboxylase and Oxygenase Activities

1981 • 137 citations

Genetic Dissection of Rubisco Structure and Function

1993 • 136 citations

Nonsense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulosebisphosphate carboxylase/oxygenase

1985 • 110 citations

Reaction intermediate partitioning by ribulose-bisphosphate carboxylases with differing substrate specificities.

1986 • 97 citations

Reduced CO2/O2 specificity of ribulose-bisphosphate carboxylase/oxygenase in a temperature-sensitive chloroplast mutant of Chlamydomonas.

1988 • 86 citations

How various factors influence the CO2/O2 specificity of ribulose-1,5-bisphosphate carboxylase/oxygenase

1992 • 76 citations

Intersubunit location of the active site of ribulose-bisphosphate carboxylase/oxygenase as determined by in vivo hybridization of site-directed mutants.

1987 • 75 citations

Chloroplast Intragenic Suppression Enhances the Low CO2/O2 Specificity of Mutant Ribulose-bisphosphate Carboxylase/Oxygenase

1989 • 74 citations

Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii

1991 • 72 citations

Site-specific mutations in a loop region of the C-terminal domain of the large subunit of ribulose bisphosphate carboxylase/oxygenase that influence substrate partitioning.

1993 • 70 citations

Abscisic acid biosynthesis in roots

1992 • 65 citations

Formation of the active site of ribulose-1,5-bisphosphate carboxylase/oxygenase by a disorder-order transition from the unactivated to the activated form.

1993 • 64 citations

Rice chloroplast DNA molecules are heterogeneous as revealed by DNA sequences of a cluster of genes

1987 • 60 citations

Cotranscription, deduced primary structure, and expression of the chloroplast-encoded rbcL and rbcS genes of the marine diatom Cylindrotheca sp. strain N1.

1991 • 55 citations

Biochemical and genetic analysis of an RuBP carboxylase/oxygenase‐deficient mutant and revertants of Chlamydomonas reinhardii

1982 • 52 citations

Preparative-scale enzymic synthesis of <scp>d</scp>-[14C]ribulose 1,5-bisphosphate

1978 • 47 citations

A role for the .epsilon.-amino group of lysine-334 of ribulose-1,5-bisphosphate carboxylase in the addition of carbon dioxide to the 2,3-enediol(ate) of ribulose 1,5-bisphosphonate

1993 • 46 citations

Essentiality of Lys-329 of ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum as demonstrated by site-directed mutagenesis

1988 • 45 citations

Heteroplasmic suppression of an amber mutation in the Chlamydomonas chloroplast gene that encodes the large subunit of ribulosebisphosphate carboxylase/oxygenase

1987 • 45 citations

Complementing amino acid substitutions within loop 6 of the .alpha./.beta.-barrel active site influence the carbon dioxide/oxygen specificity of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase

1991 • 45 citations

Enhanced CO2/O2 specificity of a site-directed mutant of ribulose-bisphosphate carboxylase/oxygenase.

1992 • 36 citations

Directed mutagenesis of chloroplast ribulosebisphosphate carboxylase/oxygenase. Substitutions at large subunit asparagine 123 and serine 379 decrease CO2/O2 specificity.

1994 • 36 citations

Complementing Substitutions within Loop Regions 2 and 3 of the .alpha./.beta.-Barrel Active Site Influence the CO2/O2 Specificity of Chloroplast Ribulose-1,5-bisphosphate Carboxylase/Oxygenase

1994 • 36 citations

Sequence of therbcL gene for the large subunit of ribulose bisphosphate carboxylase-oxygenase from petunia

1986 • 34 citations

Comments on the structure and function of the large subunit of the enzyme ribulose bisphosphate carboxylase-oxygenase

1981 • 32 citations

Pseudoreversion Substitution at Large-Subunit Residue 54 Influences the CO2/O2 Specificity of Chloroplast Ribulose-Bisphosphate Carboxylase/Oxygenase

1995 • 31 citations

Proteolysis and transition-state-analogue binding of mutant forms of ribulose-1,5-bisphosphate carboxylase/oxygenase from Chlamydomonas reinhardtii

1991 • 29 citations

Status of the Substrate Binding Sites of Ribulose Bisphosphate Carboxylase as Determined with 2-C-Carboxyarabinitol 1,5-Bisphosphate

1990 • 28 citations

Mechanistic insights provided by deletion of a flexible loop at the active site of ribulose-1,5-bisphosphate carboxylase/oxygenase

1995 • 28 citations

Serine-376 contributes to the binding of substrate by ribulose-bisphosphate carboxylase/oxygenase from Anacystis nidulans

1992 • 23 citations

Leucine 332 influences the CO₂/O₂specificity factor of ribulose-1, 5-bisphosphate carboxylase/oxygenase fromAnacystis nidulans

1993 • 20 citations

Sequence of therbcLgene for the large subunit of ribulose bisphosphate carboxytase-oxygenase from alfalfa

1986 • 17 citations

Role of phenylalanine-327 in the closure of loop 6 of ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum

1993 • 11 citations