Cholesterol and Sphingolipid Enhance the Triton X-100 Insolubility of Glycosylphosphatidylinositol-anchored Proteins by Promoting the Formation of Detergent-insoluble Ordered Membrane Domains

A new and rapid colorimetric determination of acetylcholinesterase activity

1961 • 25,114 citations

Antibodies: A laboratory manual

1989 • 4,532 citations

Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface

1992 • 2,905 citations

[69] Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane

1974 • 1,690 citations

Phase equilibria in the phosphatidylcholine-cholesterol system

1987 • 1,024 citations

Signal transducing molecules and glycosyl-phosphatidylinositol-linked proteins form a caveolin-rich insoluble complex in MDCK cells

1993 • 961 citations

The structure, biosynthesis and function of glycosylated phosphatidylinositols in the parasitic protozoa and higher eukaryotes

1993 • 896 citations

Interactions between saturated acyl chains confer detergent resistance on lipids and glycosylphosphatidylinositol (GPI)-anchored proteins: GPI-anchored proteins in liposomes and cells show similar behavior.

1994 • 688 citations

On the Origin of Sphingolipid/Cholesterol-Rich Detergent-Insoluble Cell Membranes: Physiological Concentrations of Cholesterol and Sphingolipid Induce Formation of a Detergent-Insoluble, Liquid-Ordered Lipid Phase in Model Membranes

1997 • 661 citations

Lateral diffusion in the liquid phases of dimyristoylphosphatidylcholine/cholesterol lipid bilayers: a free volume analysis

1992 • 563 citations

The glycosyl-phosphatidylinositol anchor of membrane proteins

1989 • 520 citations

Characterization of Detergent-insoluble Complexes Containing the Cellular Prion Protein and Its Scrapie Isoform

1997 • 388 citations

Lipid analysis of the glycoinositol phospholipid membrane anchor of human erythrocyte acetylcholinesterase. Palmitoylation of inositol results in resistance to phosphatidylinositol-specific phospholipase C.

1988 • 355 citations

Purification and characterization of smooth muscle cell caveolae.

1994 • 347 citations

Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 1. Solubilization of large unilamellar liposomes (prepared by reverse-phase evaporation) by Triton X-100, octyl glucoside, and sodium cholate

1988 • 346 citations

Interaction of cholesterol with various glycerophospholipids and sphingomyelin

1990 • 310 citations

Cholesterol-induced fluid-phase immiscibility in membranes.

1991 • 305 citations

The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases.

1992 • 300 citations

Structural characterization of the glycoinositol phospholipid membrane anchor of human erythrocyte acetylcholinesterase by fast atom bombardment mass spectrometry.

1988 • 280 citations

Palmitylation of an amino-terminal cysteine motif of protein tyrosine kinases p56lck and p59fyn mediates interaction with glycosyl-phosphatidylinositol-anchored proteins.

1993 • 259 citations

Glycosphingolipid-enriched, detergent-insoluble complexes in protein sorting in epithelial cells

1993 • 246 citations

Cholesterol at Different Bilayer Concentrations Can Promote or Antagonize Lateral Segregation of Phospholipids of Differing Acyl Chain Length

1996 • 224 citations

Ectoenzymes of the kidney microvillar membrane. Differential solubilization by detergents can predict a glycosyl-phosphatidylinositol membrane anchor

1988 • 216 citations

Ligand-induced protease receptor translocation into caveolae: a mechanism for regulating cell surface proteolysis of the tissue factor-dependent coagulation pathway.

1996 • 212 citations

Biosynthesis of glycosyl phosphatidylinositol membrane anchors.

1990 • 212 citations

Both Sphingolipids and Cholesterol Participate in the Detergent Insolubility of Alkaline Phosphatase, a Glycosylphosphatidylinositol-anchored Protein, in Mammalian Membranes

1995 • 200 citations

A calorimetric study of the thermotropic behavior of aqueous dispersions of natural and synthetic sphingomyelins

1976 • 183 citations

Structure of human erythrocyte acetylcholinesterase. Characterization of intersubunit disulfide bonding and detergent interaction.

1984 • 180 citations

Formation and properties of bacteriorhodopsin monomers in the non‐ionic detergents octyl‐β‐D‐glucoside and triton X‐100

1978 • 180 citations

Detergent insolubility of alkaline phosphatase during biosynthetic transport and endocytosis. Role of cholesterol.

1993 • 169 citations

Freeze-fracture identification of sterol-digitonin complexes in cell and liposome membranes.

1978 • 161 citations

Liquid-crystalline phases of cholesterol/lipid bilayers as revealed by the fluorescence of trans-parinaric acid

1995 • 143 citations

Differences in the glycolipid membrane anchors of bovine and human erythrocyte acetylcholinesterases.

1987 • 136 citations

Characterization of proteins in detergent-resistant membrane complexes from Madin-Darby canine kidney epithelial cells

1995 • 98 citations

Triton X-100-resistant membrane complexes from cultured kidney epithelial cells contain the Src family protein tyrosine kinase p62yes.

1994 • 80 citations

Sorting and Intracellular Trafficking of a Glycosylphosphatidylinositol-anchored Protein and Two Hybrid Transmembrane Proteins with the Same Ectodomain in Madin-Darby Canine Kidney Epithelial Cells

1995 • 74 citations

Effect of thermotropic phase transitions of dipalmitoylphosphatidylcholine on the formation of mixed micelles with Triton X-100

1974 • 53 citations

Alkylacylglycerol molecular species in the glycosylinositol phospholipid membrane anchor of bovine erythrocyte acetylcholinesterase

1988 • 46 citations

The influence of membrane composition on the solubilizing effects of Triton X-100

1987 • 23 citations