Construction andIn VitroProperties of SIV_macMutants with Deletions in "Nonessential" Genes

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

1989 • 3,067 citations

Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone

1986 • 2,918 citations

Importance of the nef gene for maintenance of high virus loads and for development of AIDS

1991 • 1,613 citations

The SCID-hu Mouse: Murine Model for the Analysis of Human Hematolymphoid Differentiation and Function

1988 • 1,394 citations

Transfer of a functional human immune system to mice with severe combined immunodeficiency

1988 • 1,248 citations

Protective Effects of a Live Attenuated SIV Vaccine with a Deletion in the nef Gene

1992 • 1,025 citations

Identification of the Envelope V3 Loop as the Primary Determinant of Cell Tropism in HIV-1

1991 • 871 citations

[71] Use of eukaryotic expression technology in the functional analysis of cloned genes

1987 • 834 citations

Serine phosphorylation-independent downregulation of cell-surface CD4 by nef

1991 • 760 citations

Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus

1985 • 706 citations

Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus

1985 • 572 citations

Human immunodeficiency virus type 1 Vpu protein induces rapid degradation of CD4

1992 • 553 citations

Induction of AIDS in rhesus monkeys by molecularly cloned simian immunodeficiency virus

1992 • 496 citations

The HIV A (sor) gene product is essential for virus infectivity

1987 • 475 citations

The human immunodeficiency virus type 1-specific protein vpu is required for efficient virus maturation and release

1990 • 461 citations

Vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells

1993 • 449 citations

Role of vif in replication of human immunodeficiency virus type 1 in CD4+ T lymphocytes

1992 • 447 citations

A Novel Gene of HIV-1, vpu , and Its 16-Kilodalton Product

1988 • 445 citations

Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1

1990 • 430 citations

The Simian Immunodeficiency Viruses

1990 • 387 citations

The sor Gene of HIV-1 Is Required for Efficient Virus Transmission in Vitro

1987 • 365 citations

The Complete Nucleotide Sequence of a Pathogenic Molecular Clone of Simian Immunodeficiency Virus

1990 • 335 citations

Decreased osmotic stability of dystrophin-less muscle cells from the mdx mouse

1991 • 331 citations

Transmission of HTLV-III Infection from Human Plasma to Chimpanzees: An Animal Model for AIDS

1984 • 325 citations

Downregulation of cell-surface CD4 expression by simian immunodeficiency virus Nef prevents viral super infection.

1993 • 291 citations

Characterization of infectious molecular clones of simian immunodeficiency virus (SIVmac) and human immunodeficiency virus type 2: persistent infection of rhesus monkeys with molecularly cloned SIVmac

1988 • 283 citations

Functional role of human immunodeficiency virus type 1 vpu.

1989 • 280 citations

Human Immunodeficiency Virus Infection of Human-PBL-SCID Mice

1991 • 277 citations

Identification of a determinant within the human immunodeficiency virus 1 surface envelope glycoprotein critical for productive infection of primary monocytes.

1991 • 272 citations

Molecular and biochemical analyses of human immunodeficiency virus type 1 vpu protein

1989 • 267 citations

Human immunodeficiency virus type 1 Vpu protein regulates the formation of intracellular gp160-CD4 complexes

1992 • 251 citations

Evolution of the primate lentiviruses: evidence from vpx and vpr.

1992 • 232 citations

Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus

1993 • 220 citations

Replicative and Cytopathic Potential of HTLV-III/LAV with sor Gene Deletions

1986 • 217 citations

Complex determinants of macrophage tropism in env of simian immunodeficiency virus

1992 • 208 citations

Persistent infection of chimpanzees with human T-lymphotropic virus type III/lymphadenopathy-associated virus: a potential model for acquired immunodeficiency syndrome

1986 • 201 citations

Human immunodeficiency virus as a prototypic complex retrovirus

1991 • 197 citations

CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals.

1993 • 186 citations

Mutational analysis of the human immunodeficiency virus vpr open reading frame

1989 • 183 citations

Infection of Macaca nemestrina by Human Immunodeficiency Virus Type-1

1992 • 173 citations

Identification of human immunodeficiency virus envelope gene sequences influencing viral entry into CD4-positive HeLa cells, T-leukemia cells, and macrophages

1991 • 160 citations

The human immunodeficiency virus type 2 vpr gene is essential for productive infection of human macrophages.

1990 • 159 citations

Conservation of amino-acid sequence motifs in lentivirus Vif proteins

1992 • 147 citations

Restricted replication of simian immunodeficiency virus strain 239 in macrophages is determined by env but is not due to restricted entry

1993 • 143 citations

Human immunodeficiency virus type 1 Vpu protein induces degradation of CD4 in vitro: the cytoplasmic domain of CD4 contributes to Vpu sensitivity

1993 • 134 citations

Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) genome in relation to HIV-1 and simian immunodeficiency virus SIV (AGM)

1990 • 119 citations

Nef from primary isolates of human immunodeficiency virus type 1 suppresses surface CD4 expression in human and mouse T cells

1993 • 118 citations

The vpx gene of simian immunodeficiency virus facilitates efficient viral replication in fresh lymphocytes and macrophage

1991 • 115 citations

The negative effect of human immunodeficiency virus type 1 Nef on cell surface CD4 expression is not species specific and requires the cytoplasmic domain of CD4

1993 • 114 citations

VPX mutants of HIV-2 are infectious in established cell lines but display a severe defect in peripheral blood lymphocytes.

1989 • 113 citations

A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction.

1991 • 108 citations

Effects of long terminal repeat mutations on human immunodeficiency virus type 1 replication

1989 • 105 citations

Alternative splice acceptor utilization during human immunodeficiency virus type 1 infection of cultured cells

1990 • 104 citations

Viral protein R of human immunodeficiency virus types 1 and 2 is dispensable for replication and cytopathogenicity in lymphoid cells

1989 • 103 citations

HIV with Multiple Gene Deletions as a Live Attenuated Vaccine for AIDS

1992 • 99 citations

A distinct African lentivirus from Sykes' monkeys

1993 • 99 citations

Mechanism of action of regulatory proteins encoded by complex retroviruses.

1992 • 90 citations

Generation and characterization of the human immunodeficiency virus type 1 mutants

1991 • 88 citations

Upstream U3 sequences in simian immunodeficiency virus are selectively deleted in vivo in the absence of an intact nef gene

1994 • 80 citations

Envelope glycoprotein and CD4 independence of vpu-facilitated human immunodeficiency virus type 1 capsid export

1992 • 78 citations

A specific inhibitor of cysteine proteases impairs a Vif-dependent modification of human immunodeficiency virus type 1 Env protein

1991 • 77 citations

Efficient DNA transfection and rapid assay for thymidine kinase activity and viral antigenic determinants

1982 • 77 citations

Use of Simian Immunodeficiency Viruses for AIDS Research

1989 • 76 citations

A Molecular Clone of HIV-1 Tropic and Cytopathic for Human and Chimpanzee Lymphocytes

1993 • 71 citations

Simian immunodeficiency viruses and their relationship to the human immunodeficiency viruses

1988 • 68 citations

Human immunodeficiency virus infection of human lymph nodes in the SCID-hu mouse.

1991 • 67 citations

Human immunodeficiency virus type 2 vpx protein augments viral infectivity

1991 • 64 citations

Characterization and expression of novel singly spliced RNA species of human immunodeficiency virus type 1

1990 • 61 citations

Transcomplementation of VIF- HIV-1 Mutants in CEM Cells Suggests That VIF Affects Late Steps of the Viral Life Cycle

1993 • 61 citations

Recombinational analysis of a natural noncytopathic human immunodeficiency virus type 1 (HIV-1) isolate: role of the vif gene in HIV-1 infection kinetics and cytopathicity

1991 • 58 citations

Identification of viral determinants of macrophage tropism for simian immunodeficiency virus SIVmac

1991 • 56 citations

Construction and characterization of an infectious DNA clone and of mutants of simian immunodeficiency virus isolated from the African green monkey

1990 • 49 citations

Distinctive pattern of infection and replication of HIV1 strains in blood-derived macrophages

1992 • 43 citations

Human immunodeficiency virus type 1 Vpu has a CD4- and an envelope glycoprotein-independent function

1993 • 39 citations

Downregulation of cell surface CD4 by nef

1992 • 39 citations

Association of human immunodeficiency virus type 1 envelope glycoprotein with particles depends on interactions between the third variable and conserved regions of gp120

1993 • 38 citations

Serine Phosphorylation-Independent Downregulation of Cell-Surface CD4 by nef

1991 • 38 citations

A PCR-based strategy for extensive mutagenesis of a target DNA sequence.

1993 • 37 citations

The Productive Infection of Alveolar Macrophages by Simian Immunodeficiency Virus

1989 • 31 citations

Nef Genes of SIV

1990 • 27 citations

Characterization of multiple mRNA species of simian immunodeficiency virus from macaques in a CD4+ lymphoid cell line

1991 • 24 citations

Use of Infectious Molecular Clones of Simian Immunodeficiency Virus for Pathogenesis Studies

1989 • 23 citations

Mutational Analysis of Simian Immunodeficiency Virus From African Green Monkeys and Human Immunodeficiency Virus Type 2

1990 • 14 citations

Changes in the Host Range and Growth Potential of an HIV-1 Clone Are Conferred by the vpu Gene

1993 • 13 citations

Development of Antiviral Treatment Strategies in Murine Models

1992 • 12 citations

A PCR-derived library of random point mutations within the V3 region of simian immunodeficiency virus.

1993 • 8 citations