Epitope mapping by mutagenesis distinguishes between the two tertiary structures of the histidine-containing protein HPr.

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria

1993 • 1,767 citations

Protein phosphorylation and regulation of adaptive responses in bacteria

1989 • 1,495 citations

A simple ultraviolet spectrophotometric method for the determination of protein.

1956 • 1,266 citations

Three-Dimensional Structure of an Antigen-Antibody Complex at 2.8 Å Resolution

1986 • 1,173 citations

Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template

1984 • 947 citations

ANTIBODY-ANTIGEN COMPLEXES

1990 • 853 citations

Three-dimensional structure of an antibody-antigen complex.

1987 • 615 citations

PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM

1964 • 606 citations

Three-dimensional structure of a complex of antibody with influenza virus neuraminidase

1987 • 556 citations

THE BACTERIAL PHOSPHOENOL-PYRUVATE: GLYCOSE PHOSPHOTRANSFERASE SYSTEM

1990 • 387 citations

Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria

1985 • 254 citations

The Phosphoenolpyruvate:Sugar Phosphotransferase System in Gram-Positive Bacteria: Properties, Mechanism, and Regulation

1988 • 190 citations

Sugar Transport

1971 • 140 citations

Modified assay procedures for the phosphotransferase system in enteric bacteria

1979 • 105 citations

Two-dimensional proton NMR studies of histidine-containing protein from Escherichia coli. 3. Secondary and tertiary structure as determined by NMR

1986 • 96 citations

Cyclic AMP synthesis in Escherichia coli strains bearing known deletions in the pts phosphotransferase operon

1990 • 86 citations

Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties

1980 • 73 citations

Signal transduction by the bacterial phosphotransferase system. Diauxie and the crr gene (J. Monod revisited).

1990 • 67 citations

Tertiary structure of histidine-containing protein of the phosphoenolpyruvate:sugar phosphotransferase system of Escherichia coli.

1987 • 56 citations

Characterization of phosphorylated histidine-containing protein (HPr) of the bacterial phosphoenolpyruvate/sugar phosphotransferase system

1985 • 53 citations

Two-dimensional proton NMR studies of histidine-containing protein from Escherichia coli. 1. Sequential resonance assignments

1986 • 44 citations

Structure determination of a monoclonal Fab fragment specific for histidine-containing protein of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli.

1988 • 36 citations

Characterization of mutant histidine-containing proteins of the phosphoenolpyruvate:sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium

1987 • 22 citations

Expression of the ptsH⁺ gene of Escherichia coli cloned on plasmid pBR322

1982 • 18 citations

What can two-dimensional NMR tell us about proteins?

1989 • 18 citations

Preliminary X-ray data for the HPr protein of the phosphoenolpyruvate: Sugar phosphotransferase system of Escherichia coli

1982 • 8 citations

Crystallization of the Complex of a Monoclonal Fab Fragment with the Histidine-containing Protein of the Phosphoenolpyruvate: Sugar Phosphotransferase System of Escherichia coli

1989 • 6 citations