The use of inhibitors in the study of glycosidases

Methods in Enzymology , Vol

1966 • 26,261 citations

Esters of Methanesulfonic Acid as Irreversible Inhibitors of Acetylcholinesterase

1962 • 1,510 citations

Kinetic Aspects of Regulation of Metabolic Processes

1970 • 616 citations

Mechanism in carbohydrate chemistry

1969 • 574 citations

THE BETA- <scp>d</scp> -GALACTOSIDASE OF ESCHERICHIA COLI, STRAIN K-12

1950 • 393 citations

Design of Active-Site-Directed Irreversible Enzyme Inhibitors.

1967 • 387 citations

Structure and synthesis of nojirimycin

1968 • 366 citations

Inhibition of lysosomal α-mannosidase by swainsonine, an indolizidine alkaloid isolated from Swainsona canescens

1980 • 365 citations

Chemistry and Enzymology of k _cat Inhibitors

1974 • 283 citations

A Method for Characterizing the Type and Numbers of Groups Involved in Enzyme Action

1961 • 236 citations

X-Ray Diffraction Studies of Enzymes

1970 • 194 citations

Amino acid sequence of beta-galactosidase. XI. Peptide ordering procedures and the complete sequence

1978 • 191 citations

Nojirimycin and d-glucono-1,5-lactone as inhibitors of carbohydrases

1971 • 190 citations

Regulatory Behavior of Monomeric Enzymes

1974 • 179 citations

Competitive Inhibition by Substrate during Enzyme Action. Evidence for the Induced-fit Theory^1,2

1960 • 167 citations

Chemie und Biochemie mikrobieller α‐Glucosidasen‐Inhibitoren

1981 • 146 citations

The mechanism of action of β-galactosidase. Effect of aglycone nature and α-deuterium substitution on the hydrolysis of aryl galactosides

1973 • 140 citations

464. The reactions of certain epoxides in aqueous solutions

1950 • 132 citations

The Allosteric Activation of Mammalian α‐Amylase by Chloride

1974 • 131 citations

Glucosidases and exo-glucanases

1968 • 130 citations

pH Dependence of the Activity of β‐Galactosidase from Escherichia coli

1971 • 119 citations

An example of enzyme hysteresis. The slow and tight interaction of some fully competitive inhibitors with small intestinal sucrase.

1981 • 112 citations

A study of the kinetics of the reaction between thiol compounds and chloroacetamide

1960 • 103 citations

Reversible alkylation of a methionyl residue near the active site of β-galactosidase

1972 • 98 citations

β‐Glucosidases from Cicer arietinum L.

1975 • 96 citations

The Gaucher mouse

1975 • 94 citations

D-glucosyl isothiocyanate, an affinity label for the glucose transport proteins of the human erythrocyte membrane

1973 • 93 citations

Epoxyalkyl oligo-(1→4)-β-D-glucosides as active-site-directed inhibitors of cellulases

1973 • 89 citations

Untersuchungen zum Wirkungsmechanismus glykosidspaltender Enzyme, III. Markierung des aktiven Zentrums einer β-Glucosidase ausAspergillus wentiimit [¹⁴C]Condurit-B-epoxid

1968 • 86 citations

The three-dimensional structure of α-amylase from porcine pancreas at 5 Å resolution – the active-site location

1980 • 85 citations

Scope and mechanism of carbohydrase action: stereospecific hydration of D-glucal catalyzed by α- and β-glucosidase

1977 • 85 citations

Slow binding of D-galactal, a reversible inhibitor of bacterial β-galactosidase

1974 • 82 citations

Partial amino acid sequences around the essential carboxylate in the active sites of the intestinal sucrase-isomaltase complex.

1976 • 81 citations

Conformational adaptability of the active site of beta-galactosidase. Interaction of the enzyme with some substrate analogous effectors.

1978 • 80 citations

Steady‐State Kinetics of Rabbit‐Intestinal Sucrase

1974 • 80 citations

Kinetic Study of the Activation Process of β‐Galactosidase from Escherichia coli by Mg²⁺

1972 • 78 citations

1-Aminoglycosides, a new class of specific inhibitors of glycosidases

1973 • 72 citations

Untersuchungen zum Wirkungsmechanismus glykosidspaltender Enzyme, I. Darstellung und Eigenschaften spezifischer Inaktivatoren

1966 • 72 citations

“Nojirimycin” as a Potent Inhibitor of Glucosidase

1970 • 70 citations

Isotopic effects and inhibition of polysaccharide phosphorylase by 1,5-gluconolactone. Relation to the catalytic mechanism

1971 • 68 citations

The Reaction of Glutamate Dehydrogenase with 4‐Iodoacetamido Salicylic Acid

1970 • 67 citations

Inhibition of β-D-galactosidases by D-galactal

1969 • 65 citations

Inhibition of glycosidases by aldonolactones of corresponding configuration. 3. Inhibitors of β-<scp>d</scp>-galactosidase

1962 • 64 citations

Specific and Irreversible Inhibition of Lysozyme by 2′,3′-Epoxypropyl β-Glycosides of N-Acetyl-D-glucosamine Oligomers

1969 • 62 citations

The conversion of 2,6-anhydro-1-deoxy-d-galacto-hept-1-enitol into 1-deoxy-d-galacto-heptulose by β-d-galactosidase

1977 • 62 citations

Scope and mechanism of carbohydrase action. Stereospecific hydration of 2,6-anhydro-1-deoxy-D-gluco-hept-1-enitol catalyzed by .alpha.- and .beta.-glucosidases and an inverting exo-.alpha.-glucanase

1980 • 60 citations

Active site‐directed inhibition of galactosidases by conduritol C epoxides (1,2‐anhydro‐EPI‐and NEO‐inositol

1981 • 60 citations

Inhibition of glycosidases by aldonolactones of corresponding configuration. 4. Inhibitors of mannosidase and glucosidase

1964 • 60 citations

Reaktionen enolischer zuckerderivate

1972 • 60 citations

Affinity Labeling of the Active Sites in the Sucrase-Isomaltase Complex from Small Intestine

1974 • 59 citations

Evidence for a single catalytic site on the “β3-d-glucosidase-β-d-galactosidase” of almond emulsin

1978 • 59 citations

Active site directed inhibitors and mechanism of action of glycosidases

1973 • 59 citations

Role of sugar hydroxyl groups in glycoside hydrolysis. Cleavage mechanism of deoxyglucosides and related substrates by β-glucosidase A3 from Aspergillus wentii

1981 • 59 citations

Studies on almond emulsin β-d-glucosidase II. Kinetic evidence for independent glucosidase and galactosidase sites

1977 • 58 citations

The synthesis of diastereomeric epoxy(6-D-glucopyranosyl)ethanes and 1,2-epoxy-3-(β-D-glucopyranosyl)propanes, as irreversible inhibitors of β-D-glucosidase

1974 • 58 citations

Affinity labelling with a deaminatively generated carbonium ion. Kinetics and stoicheiometry of the alkylation of methionine-500 of the lacZ β-galactosidase of Escherichia coli by β-<scp>d</scp>-galactopyranosylmethyl-p-nitrophenyltriazene

1978 • 56 citations

Neue Enzyminhibitoren aus Mikroorganismen

1979 • 54 citations

Enzyme Memory

1977 • 51 citations

Untersuchungen zum Wirkungsmechanismus glykosidspaltender Enzyme, VII. Funktionelle Gruppen am aktiven Zentrum einer α-Glucosidase ausSaccharomyces cerevisiae

1973 • 51 citations

Reaction of β‐<scp>d</scp>‐Glucosidase A₃ from Aspergillus wentii with <scp>d</scp>‐Glucal

1979 • 51 citations

Inhibition of β-glucosidases from almonds by cationic and neutral β-glucosyl derivatives

1978 • 50 citations

Isolation and Amino Acid Sequence of a Hexadecapeptide from the Active Site of ß-Glucosidase A₃ from Aspergillus wentii

1974 • 50 citations

Nucleophilic Competition in Some β‐Galactosidase‐Catalyzed Reactions

1973 • 50 citations

Mechanism for lysozyme-catalyzed hydrolysis

1973 • 49 citations

Stereoselective syntheses of O- and S-nitrophenyl glycosides. Part III. Syntheses in the α-<scp>D</scp>-galactopyranose and α-maltose series

1981 • 47 citations

Debromination of Inositol Bromohydrins.^* Synthesis of “Conduritol-B,” scylloQuercitol, and D,L-viboQuercitol^1,2

1953 • 46 citations

A possible mechanism for amylase catalysis

1968 • 46 citations

Transient nuclear magnetic resonance study of the kinetics of methyl N-acetyl-D-glucosaminide inhibition of lysozyme

1969 • 44 citations

Amino acid sequence at the active site of β-glucosidase a from bitter almonds

1978 • 44 citations

Role of strain in catalysis by lysozyme

1971 • 44 citations

Specific inactivation of lysosomal glycosidases in living fibroblasts by the corresponding glycosylmethyl-p-nitrophenyltriazenes

1980 • 43 citations

THE GAUCHER MOUSE: DIFFERENTIAL ACTION OF CONDURITOL B EPOXIDE AND REVERSIBILITY OF ITS EFFECTS

1978 • 40 citations

Determination of the anomeric configuration of D-xylose with D-xylose isomerases

1976 • 39 citations

Destruction and resynthesis of mouse β-glucosidases

1979 • 38 citations

Zur Chemie des Benzolglykols, V. Eine neue Synthese der Inosite

1959 • 38 citations

Identity and properties of the chloride effector binding site in hog pancreatic α-amylase

1976 • 37 citations

Specific irreversible inhibition of sweet-almond β-glucosidase by some β-glycopyranosylepoxyalkanes and β-d-glucopyranosyl isothiocyanate

1976 • 37 citations

Binding of alkyl 1-Thio-β-d-galactopyranosides to β-d-galactosidase from E. coli

1977 • 37 citations

Labelling of the active site of β‐galactosidase by N‐bromoacetyl β‐D‐galactopyranosylamine

1971 • 35 citations

Specific and irreversible lysozyme inhibitors: 2′,3′-epoxypropyl β-d-glycosides of 2-acetamido-2-deoxy-d-glucose and its oligomers

1970 • 34 citations

Methionine 500, the site of covalent attachment of an active site-directed reagent of beta-galactosidase

1978 • 34 citations

Stereospecific ring opening of conduritol-B-epoxide by an active site aspartate residue of sucrase-isomaltase

1977 • 33 citations

Mutarotation, hydrolysis, and structure of D-galactosylamines

1951 • 33 citations

Thermodynamics and kinetics of D-galactose tautomers during mutarotation

1969 • 32 citations

Identification of Aspartic Acid 52 as the Point of Attachment of an Affinity Label in Hen Egg White Lysozyme

1973 • 31 citations

The identification by X-ray crystallography of the site of attachment of an affinity label to hen egg-white lysozyme

1973 • 29 citations

Studies on the Subsite Structure of Amylases

1975 • 28 citations

Affinity Labelling of Rat‐Muscle Hexokinase Type II by a Glucose‐Derived Alkylating Agent

1979 • 27 citations

Interaction of the lacZ β-galactosidase of Escherichia coli with some β-<scp>d</scp>-galactopyranoside competitive inhibitors

1979 • 26 citations

A study by means of lactone inhibition of the role of a “half-chair” glycosyl conformation at the active centre of amylolytic enzymes

1978 • 26 citations

Potential specific inhibitors of the lactose transport system of Escherichia coli

1970 • 26 citations

Kinetics of lysozyme-substrate interactions

1969 • 25 citations

Stepwise binding of small molecules to proteins. Nuclear magnetic resonance and temperature jump studies of the binding of 4-(N-acetylaminoglucosyl)-N-acetylglucosamine to lysozyme

1975 • 24 citations

The synthesis of 1L-1,2-anhydro-myo-inositol and 1L- and DL-1,2-anhydro-chiro-inositol

1971 • 18 citations

A Study of the Kinetics of the Hydrolysis of Waxy Maize Starch by Crystalline Pancreatic Amylase from Swine¹

1956 • 18 citations

Comparison of the .beta.-galactosidase conformations induced by D-galactal and by magnesium ions

1980 • 18 citations

Intermediate steps in the incorporation of fatty acids into long-chain, nonisoprenoid hydrocarbons by lysates of Sarcina lutea

1970 • 16 citations

Dependence upon pH of Steady‐State Parameters for the β‐Galactosidase‐Catalysed Hydrolyses of β‐D‐Galactopyranosyl Derivatives of Different Chemical Types

1978 • 16 citations

External yeast β-fructosidase. Affinity labeling of the active site

1976 • 15 citations

The inactivation of β‐galactosidase by N‐bromoacetyl‐β‐D‐glucosylamine

1977 • 14 citations

The preparation of 2,3-epoxypropyl β-D-glucopyranoside

1971 • 12 citations

Affinity labelling of β-D-galactosidase from Escherichia coli with d-[6-3H]-galactal

1981 • 10 citations

External yeast β-fructosidase: Stereospecific labeling by [3H]conduritol-B-epoxide and isolation of labeled peptides from the active site

1977 • 10 citations

β-<scp>D</scp>-Xylosidase from Penicillium wortmanni: binding and hydrolysis of alkyl and aryl 1-oxygen and 1-thio-β-<scp>D</scp>-xylopyranosides

1980 • 10 citations

Untersuchungen zum Wirkungsmechanismus glykosidspaltender Enzyme, VIII. Anzahl der aktiven Zentren der β - Glucosidasen A und B aus dem Süßmandel-Emulsin durch Fluoreszenzmessungen

1974 • 10 citations

Binding of η-alkyl-β-D-xylopyranosides and η-alkyl 1-thio-β-D-xylopyranosides to β-D-xylosidase from Bacillus pumilus PRL B12

1980 • 9 citations

Inhibition of human liver β-galactosidase and β-glucosidase by

1975 • 9 citations

La β-glucosidase de l'amande douce (Amygdalus Communis L.)

1974 • 9 citations

Spezifischeβ-Glucosidasen für das cyanogene Glucosid Triglochinin ausAlocasia macrorrhizaSchott. Weitere Charakterisierung der Eigenschaften

1977 • 9 citations

Binding of substituted phenyl 1-thio-β-D-galactopyranosides to β-D-galactosidase from e. coli

1978 • 8 citations

STUDIEN ÜBER DIE CARBOHYDRASE

1940 • 7 citations

STUDIEN ÜBER DIE CARBOHYDRASE

1942 • 7 citations

Notiz über die Bildung des Bittermandelöls

1837 • 5 citations

The effective charges at the active sites of two glycosidases

1974 • 5 citations

Effect of adenosine triphosphate on beef cardiac actomyosin

1971 • 4 citations

Inhibitors of β-Glucosidases of Animal Tissues

1978 • 3 citations