Structures of Larger Proteins in Solution: Three- and Four-Dimensional Heteronuclear NMR Spectroscopy

Investigation of exchange processes by two-dimensional NMR spectroscopy

1979 • 4,720 citations

Relaxation Processes in a System of Two Spins

1955 • 3,133 citations

Two-dimensional spectroscopy. Application to nuclear magnetic resonance

1976 • 3,023 citations

The relation between the divergence of sequence and structure in proteins.

1986 • 2,373 citations

Vicinal Proton Coupling in Nuclear Magnetic Resonance

1963 • 2,349 citations

Polarization of Nuclei in Metals

1953 • 1,610 citations

Traitement statistique des erreurs dans la determination des structures cristallines

1952 • 1,284 citations

Calibration of the angular dependence of the amide proton-Cα proton coupling constants, 3JHNα, in a globular protein

1984 • 956 citations

Overcoming the overlap problem in the assignment of proton NMR spectra of larger proteins by use of three-dimensional heteronuclear proton-nitrogen-15 Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1.beta.

1989 • 840 citations

A novel approach for sequential assignment of proton, carbon-13, and nitrogen-15 spectra of larger proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin

1990 • 836 citations

Determination of three‐dimensional structures of proteins from interproton distance data by hybrid distance geometry‐dynamical simulated annealing calculations

1988 • 775 citations

Knowledge-based prediction of protein structures and the design of novel molecules

1987 • 653 citations

Solution conformation of proteinase inhibitor IIA from bull seminal plasma by 1H nuclear magnetic resonance and distance geometry

1985 • 610 citations

Three-dimensional heteronuclear NMR of nitrogen-15 labeled proteins

1989 • 584 citations

1H1H correlation via isotropic mixing of 13C magnetization, a new three-dimensional approach for assigning 1H and 13C spectra of 13C-enriched proteins

1990 • 580 citations

Protein structures in solution by nuclear magnetic resonance and distance geometry

1987 • 569 citations

Calculation of protein conformations by proton-proton distance constraints

1985 • 564 citations

Determination of three‐dimensional structures of proteins from interproton distance data by dynamical simulated annealing from a random array of atoms Circumventing problems associated with folding

1988 • 546 citations

Determination of three-dimensional structures of proteins by simulated annealing with interproton distance restraints. Application to crambin, potato carboxypeptidase inhibitor and barley serine proteinase inhibitor 2

1988 • 537 citations

Determination of the three-dimensional solution structure of the C-terminal domain of cellobiohydrolase I from Trichoderma reesei. A study using nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing

1989 • 513 citations

The structure of the Antennapedia homeodomain determined by NMR spectroscopy in solution: Comparison with prokaryotic repressors

1989 • 468 citations

A protein structure from nuclear magnetic resonance data

1985 • 464 citations

Protein Structure Determination in Solution by Nuclear Magnetic Resonance Spectroscopy

1989 • 444 citations

Three-dimensional structure of interleukin 8 in solution

1990 • 441 citations

Crystallographic refinement by simulated annealing

1988 • 411 citations

Heteronuclear three-dimensional nmr spectroscopy. A strategy for the simplification of homonuclear two-dimensional NMR spectra

1988 • 382 citations

The theory and practice of distance geometry

1983 • 377 citations

An evaluation of the combined use of nuclear magnetic resonance and distance geometry for the determination of protein conformations in solution

1985 • 373 citations

Paramagnetic Relaxation in Metals

1953 • 362 citations

Crystal structure of interleukin 8: symbiosis of NMR and crystallography.

1991 • 354 citations

Protein structure determination in solution by NMR spectroscopy.

1990 • 352 citations

Determination of Three-Dimensional Structures of Proteins and Nucleic Acids in Solution by Nuclear Magnetic Resonance Spectroscop

1989 • 342 citations

An efficient 3D NMR technique for correlating the proton and15N backbone amide resonances with the α-carbon of the preceding residue in uniformly15N/13C enriched proteins

1991 • 330 citations

Computer-Aided Molecular Design

1987 • 324 citations

Heteronuclear three-dimensional NMR spectroscopy of the inflammatory protein C5a

1989 • 324 citations

Three-dimensional structure of proteins determined by molecular dynamics with interproton distance restraints: application to crambin.

1986 • 322 citations

Four-Dimensional Heteronuclear Triple-Resonance NMR Spectroscopy of Interleukin-1β in Solution

1990 • 308 citations

Three-dimensional structure of rabbit liver [Cd7]metallothionein-2a in aqueous solution determined by nuclear magnetic resonance

1988 • 304 citations

Solvent suppression using a spin lock in 2D and 3D NMR spectroscopy with H2O solutions

1989 • 290 citations

New methods for the measurement of NHCαH coupling constants in 15N-labeled proteins

1990 • 262 citations

P.E.COSY, a simple alternative to E.COSY

1987 • 257 citations

Application of molecular dynamics with interproton distance restraints to three-dimensional protein structure determination

1986 • 252 citations

The three-dimensional structure of α1-purothionin in solution: combined use of nuclear magnetic resonance, distance geometry and restrained molecular dynamics

1986 • 250 citations

Solution conformation of a heptadecapeptide comprising the DNA binding helix F of the cyclic AMP receptor protein of Escherichia coli

1985 • 248 citations

Three-dimensional NMR spectroscopy of a protein in solution

1988 • 240 citations

Determination of the complete three-dimensional structure of the α-amylase inhibitor tendamistat in aqueous solution by nuclear magnetic resonance and distance geometry

1988 • 231 citations

Practical aspects of 3D heteronuclear NMR of proteins

1989 • 222 citations

Distance geometry and related methods for protein structure determination from NMR data

1987 • 215 citations

Protein structures from NMR

1988 • 212 citations

Automated stereospecific proton NMR assignments and their impact on the precision of protein structure determinations in solution

1989 • 212 citations

Three-dimensional NOESY-HMQC spectroscopy of a 13C-labeled protein

1990 • 212 citations

Practical aspects of proton-carbon-carbon-proton three-dimensional correlation spectroscopy of 13C-labeled proteins

1990 • 200 citations

Assignment of the side-chain proton and carbon-13 resonances of interleukin-1.beta. using double- and triple-resonance heteronuclear three-dimensional NMR spectroscopy

1990 • 194 citations

Proton-proton correlation via carbon-carbon couplings: a three-dimensional NMR approach for the assignment of aliphatic resonances in proteins labeled with carbon-13

1990 • 189 citations

Stereospecific assignments of side‐chain protons and characterization of torsion angles in Eglin c

1987 • 187 citations

TWO-DIMENSIONAL NMR AND PROTEIN STRUCTURE

1989 • 183 citations

High-resolution three-dimensional structure of interleukin 1.beta. in solution by three- and four-dimensional nuclear magnetic resonance spectroscopy

1991 • 175 citations

Effect of anisotropy and anharmonicity on protein crystallographic refinement

1986 • 172 citations

A powerful method of sequential proton resonance assignment in proteins using relayed ¹⁵N‐¹H multiple quantum coherence spectroscopy

1989 • 172 citations

Complete resonance assignment for the polypeptide backbone of interleukin 1.beta. using three-dimensional heteronuclear NMR spectroscopy

1990 • 168 citations

2D and 3D NMR spectroscopy employing carbon-13/carbon-13 magnetization transfer by isotropic mixing. Spin system identification in large proteins

1990 • 168 citations

Stable calculation of coordinates from distance information

1978 • 163 citations

Four-dimensional carbon-13/carbon-13-edited nuclear Overhauser enhancement spectroscopy of a protein in solution: application to interleukin 1.beta.

1991 • 162 citations

Detection of nuclear Overhauser effects between degenerate amide proton resonances by heteronuclear three-dimensional NMR spectroscopy

1990 • 162 citations

Three-dimensional solution structure of the reduced form of Escherichia coli thioredoxin determined by nuclear magnetic resonance spectroscopy

1990 • 160 citations

Determination of three-dimensional structures of proteins in solution by nuclear magnetic resonance spectroscopy

1987 • 159 citations

Solution structure of recombinant hirudin and the Lys-47 .fwdarw. Glu mutants: a nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing study

1989 • 155 citations

Analysis of the ¹H‐NMR spectra of ferrichrome peptides. I. The non‐amide protons

1978 • 154 citations

Two-, Three-, and Four-Dimensional NMR Methods for Obtaining Larger and More Precise Three-Dimensional Structures of Proteins in Solution

1991 • 151 citations

Structure refinement of oligonucleotides by molecular dynamics with nuclear overhauser effect interproton distance restraints: Application to 5′ d(C-G-T-A-C-G)2

1986 • 148 citations

A practical approach to three-dimensional NMR spectroscopy

1987 • 146 citations

Three-dimensional triple-resonance NMR of 13C/15N-enriched proteins using constant-time evolution

1991 • 142 citations

High-resolution three-dimensional structure of reduced recombinant human thioredoxin in solution

1991 • 141 citations

Identification and localization of bound internal water in the solution structure of interleukin 1.beta. by heteronuclear three-dimensional proton rotating-fram Overhauser nitrogen-15-proton multiple quantum coherence NMR spectroscopy

1990 • 141 citations

Two-Dimensional Nuclear Magnetic Resonance Spectroscopy

1986 • 137 citations

Determination of the three-dimensional solution structure of the antihypertensive and antiviral protein BDS-I from the sea anemone Anemonia sulcata: a study using nuclear magnetic resonance and hybrid distance geometry-dynamical simulated annealing

1989 • 135 citations

High-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solution

1990 • 128 citations

Conformation of recombinant desulfatohirudin in aqueous solution determined by nuclear magnetic resonance

1989 • 126 citations

P.COSY, a sensitive alternative for double-quantum-filtered COSY

1988 • 119 citations

Determination of the three-dimensional structure of theAntennapedia homeodomain fromDrosophila in solution by1H nuclear magnetic resonance spectroscopy

1990 • 117 citations

Stereospecific assignment of β-methylene protons in larger proteins using 3D15N-separated Hartmann-Hahn and13C-separated rotating frame Overhauser spectroscopy

1991 • 105 citations

Simulated Annealing in Crystallography

1991 • 104 citations

HMQC-NOESY-HMQC, a three-dimensional NMR experiment which allows detection of nuclear overhauser effects between protons with overlapping signals

1990 • 104 citations

[10] Molecular dynamics simulation techniques for determination of molecular structures from nuclear magnetic resonance data

1989 • 100 citations

¹H‐Nmr stereospecific assignments by conformational data‐base searches

1990 • 100 citations

Three-dimensional 13C-resolved proton NOE spectroscopy of uniformly 13C-labeled proteins for the NMR assignment and structure determination of larger molecules

1990 • 97 citations

Measurement of 1H-1H coupling constants in DNA fragments by 2D NMR

1988 • 94 citations

The design and optimization of complex NMR experiments. Application to a triple-resonance pulse scheme correlating Hα, NH, and 15N chemical shifts in 15N13C-labeled proteins

1991 • 92 citations

Four-dimensional [13C,1H,13C,1H] HMQC-NOE-HMQC NMR spectroscopy: resolving tertiary nuclear Overhauser effect distance constraints in the spectra of larger proteins

1991 • 89 citations

[9] Distance geometry

1989 • 84 citations

Studies on the solution conformation of human thioredoxin using heteronuclear nitrogen-15-proton nuclear magnetic resonance spectroscopy

1990 • 83 citations

Determination of the secondary structure and molecular topology of interleukin-1.beta. by use of two- and three-dimensional heteronuclear nitrogen-15-proton NMR spectroscopy

1990 • 63 citations

Comparison of the solution nuclear magnetic resonance and crystal structures of interleukin-8

1991 • 56 citations

The influence of stereospecific assignments on the determination of three‐dimensional structures of proteins by nuclear magnetic resonance spectroscopy

1989 • 55 citations

THE NUCLEAR OVERHAUSER EFFECT IN RIGID MOLECULES

1971 • 53 citations

Analysis of the relative contributions of the nuclear Overhauser interproton distance restraints and the empirical energy function in the calculation of oligonucleotide structures using restrained molecular dynamics

1989 • 52 citations

[2] Two-dimensional nuclear magnetic resonance spectroscopy of proteins: An overview

1989 • 52 citations

Protein structure determination in solution by two-dimensional and three-dimensional nuclear magnetic resonance spectroscopy

1990 • 35 citations

Low resolution structure of interleukin-1β in solution derived from 1H15N heteronuclear three-dimensional nuclear magnetic resonance spectroscopy

1990 • 24 citations